Buchnera aphidicola (Nipponaphis monzeni)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Buchnera; Buchnera aphidicola

Average proteome isoelectric point is 8.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 441 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A455TA08|A0A455TA08_9GAMM NADH-quinone oxidoreductase subunit N OS=Buchnera aphidicola (Nipponaphis monzeni) OX=2495405 GN=nuoN PE=3 SV=1
MM1 pKa = 7.31KK2 pKa = 10.05PEE4 pKa = 3.88EE5 pKa = 4.11KK6 pKa = 10.71ALIEE10 pKa = 4.44SLFNRR15 pKa = 11.84LKK17 pKa = 10.82VIEE20 pKa = 4.37EE21 pKa = 3.97KK22 pKa = 10.84FPNRR26 pKa = 11.84NRR28 pKa = 11.84LVSEE32 pKa = 4.5YY33 pKa = 10.31ISSLVKK39 pKa = 10.39NQPNSIYY46 pKa = 10.74YY47 pKa = 9.63IAQTTLIQEE56 pKa = 4.27VAIKK60 pKa = 10.38KK61 pKa = 9.55LNKK64 pKa = 9.7KK65 pKa = 9.48ILYY68 pKa = 9.28LEE70 pKa = 4.44KK71 pKa = 10.45KK72 pKa = 10.1ISDD75 pKa = 4.67LISQKK80 pKa = 7.54NHH82 pKa = 6.81KK83 pKa = 9.36EE84 pKa = 3.72PSFLSSLFGSSKK96 pKa = 10.38KK97 pKa = 10.34SKK99 pKa = 8.81KK100 pKa = 7.22TQNFEE105 pKa = 4.24NNNFEE110 pKa = 4.08TSAIPNSGNNNLKK123 pKa = 10.41ALNNSSPVSSLGNSFIGNALQTAAGVAGGIVVGNMLMNLFHH164 pKa = 7.26KK165 pKa = 10.28DD166 pKa = 3.21KK167 pKa = 10.76PEE169 pKa = 3.71EE170 pKa = 4.28EE171 pKa = 4.18IFDD174 pKa = 4.66TIQEE178 pKa = 4.33DD179 pKa = 3.96ATFSNLEE186 pKa = 4.13SYY188 pKa = 11.11DD189 pKa = 4.02FNNKK193 pKa = 9.44DD194 pKa = 3.29QFLSTSEE201 pKa = 4.21DD202 pKa = 3.5FLVNHH207 pKa = 7.62DD208 pKa = 4.45DD209 pKa = 3.72SQLNSVTEE217 pKa = 4.12TLDD220 pKa = 3.44QEE222 pKa = 4.51ILEE225 pKa = 4.72TEE227 pKa = 3.94HH228 pKa = 6.83DD229 pKa = 4.09TNNVIDD235 pKa = 3.84NQYY238 pKa = 11.23YY239 pKa = 10.61DD240 pKa = 5.36DD241 pKa = 4.58SDD243 pKa = 4.0NNSFDD248 pKa = 3.71NATLDD253 pKa = 6.12DD254 pKa = 4.23IDD256 pKa = 5.53DD257 pKa = 4.65DD258 pKa = 4.57NFII261 pKa = 5.11

Molecular weight: 29.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A455TAQ2|A0A455TAQ2_9GAMM Pyridoxal phosphate homeostasis protein OS=Buchnera aphidicola (Nipponaphis monzeni) OX=2495405 GN=yggS PE=3 SV=1
MM1 pKa = 7.06STINQLVRR9 pKa = 11.84KK10 pKa = 9.64SRR12 pKa = 11.84IKK14 pKa = 10.51KK15 pKa = 9.17IIKK18 pKa = 9.96SSVPALEE25 pKa = 4.25SCPQKK30 pKa = 10.56RR31 pKa = 11.84GVCIKK36 pKa = 10.8VYY38 pKa = 7.65TTTPKK43 pKa = 10.53KK44 pKa = 10.26PNSALRR50 pKa = 11.84KK51 pKa = 7.38VCRR54 pKa = 11.84VRR56 pKa = 11.84LTNGFEE62 pKa = 3.93VTSYY66 pKa = 10.74IGGEE70 pKa = 3.94GHH72 pKa = 6.6NLQEE76 pKa = 4.47HH77 pKa = 5.28SVILIRR83 pKa = 11.84GGRR86 pKa = 11.84VKK88 pKa = 10.7DD89 pKa = 3.73LPGVRR94 pKa = 11.84YY95 pKa = 9.07HH96 pKa = 7.16VIRR99 pKa = 11.84GSLDD103 pKa = 3.24CAGVKK108 pKa = 9.52DD109 pKa = 4.26RR110 pKa = 11.84KK111 pKa = 10.17NGRR114 pKa = 11.84SKK116 pKa = 11.31YY117 pKa = 9.07GVKK120 pKa = 10.12KK121 pKa = 10.65VRR123 pKa = 11.84SS124 pKa = 3.65

Molecular weight: 13.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

441

0

441

142382

38

1405

322.9

36.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.093 ± 0.088

1.393 ± 0.035

3.71 ± 0.072

4.46 ± 0.102

4.836 ± 0.097

5.226 ± 0.107

2.18 ± 0.052

12.272 ± 0.128

10.228 ± 0.133

10.048 ± 0.118

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.203 ± 0.046

8.06 ± 0.105

3.02 ± 0.06

3.422 ± 0.051

3.278 ± 0.082

6.753 ± 0.072

4.947 ± 0.065

5.014 ± 0.082

0.901 ± 0.044

3.956 ± 0.072
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski