Vibrio phage VP-HS15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Colwellvirinae; Kaohsiungvirus; unclassified Kaohsiungvirus

Average proteome isoelectric point is 5.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9LT58|A0A6B9LT58_9CAUD Uncharacterized protein OS=Vibrio phage VP-HS15 OX=2686284 PE=4 SV=1
MM1 pKa = 7.73DD2 pKa = 4.72VEE4 pKa = 5.27IIDD7 pKa = 3.95YY8 pKa = 11.06NSTDD12 pKa = 3.54VVDD15 pKa = 3.92LTGGKK20 pKa = 7.73TEE22 pKa = 4.58PEE24 pKa = 4.09DD25 pKa = 3.48PKK27 pKa = 10.94QEE29 pKa = 4.02QEE31 pKa = 4.25PEE33 pKa = 3.94QSTVTEE39 pKa = 4.0PVEE42 pKa = 3.96PAEE45 pKa = 4.49EE46 pKa = 4.64SPASTPAEE54 pKa = 4.22DD55 pKa = 5.0ADD57 pKa = 3.97AEE59 pKa = 4.48SAATEE64 pKa = 4.12PEE66 pKa = 4.02KK67 pKa = 10.57TEE69 pKa = 3.75QEE71 pKa = 4.15EE72 pKa = 4.46PEE74 pKa = 4.16EE75 pKa = 4.41SGEE78 pKa = 4.0KK79 pKa = 10.0PEE81 pKa = 4.19YY82 pKa = 10.21FFNGEE87 pKa = 4.03AVSVEE92 pKa = 4.04VPEE95 pKa = 4.28EE96 pKa = 3.63VRR98 pKa = 11.84NALKK102 pKa = 10.28EE103 pKa = 3.98AEE105 pKa = 4.12IDD107 pKa = 3.74EE108 pKa = 4.62KK109 pKa = 11.59ALLSQLFKK117 pKa = 10.97KK118 pKa = 10.75DD119 pKa = 3.18GDD121 pKa = 3.77FSLDD125 pKa = 3.12EE126 pKa = 4.0EE127 pKa = 4.82TRR129 pKa = 11.84TKK131 pKa = 10.96LEE133 pKa = 4.21SKK135 pKa = 10.39FGKK138 pKa = 8.56TLVDD142 pKa = 4.87GYY144 pKa = 11.49LNMYY148 pKa = 10.45KK149 pKa = 10.57GMNEE153 pKa = 3.91QAIKK157 pKa = 10.39AAAADD162 pKa = 3.97KK163 pKa = 9.71EE164 pKa = 4.37QQEE167 pKa = 4.71VTSKK171 pKa = 10.1QQAEE175 pKa = 4.21EE176 pKa = 4.1FMTTVGGEE184 pKa = 4.03EE185 pKa = 4.36GLVAMEE191 pKa = 5.82DD192 pKa = 4.36FILKK196 pKa = 10.28NFDD199 pKa = 3.74DD200 pKa = 4.43KK201 pKa = 11.56QIAAYY206 pKa = 8.87NAVMEE211 pKa = 4.7SEE213 pKa = 4.2SHH215 pKa = 5.2EE216 pKa = 4.04QQLLIIQTVKK226 pKa = 11.12AQMEE230 pKa = 4.37LSDD233 pKa = 4.3KK234 pKa = 10.6LQNGDD239 pKa = 4.13KK240 pKa = 10.54NIKK243 pKa = 10.25LLGDD247 pKa = 4.04DD248 pKa = 4.16TSSTPTGTPLDD259 pKa = 3.86KK260 pKa = 11.11GYY262 pKa = 8.86LTSDD266 pKa = 3.7EE267 pKa = 4.59FQAIMDD273 pKa = 3.96SDD275 pKa = 4.22KK276 pKa = 10.55YY277 pKa = 8.96WTDD280 pKa = 3.51FEE282 pKa = 4.7YY283 pKa = 9.48QTKK286 pKa = 10.19VDD288 pKa = 3.71AARR291 pKa = 11.84SAGLKK296 pKa = 10.43RR297 pKa = 11.84NLL299 pKa = 3.73

Molecular weight: 33.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LQ90|A0A6B9LQ90_9CAUD Uncharacterized protein OS=Vibrio phage VP-HS15 OX=2686284 PE=4 SV=1
MM1 pKa = 7.4TEE3 pKa = 3.58LRR5 pKa = 11.84KK6 pKa = 10.08AISSRR11 pKa = 11.84AVKK14 pKa = 10.61HH15 pKa = 6.3KK16 pKa = 10.69DD17 pKa = 3.49NPDD20 pKa = 3.27NLPNIKK26 pKa = 10.08KK27 pKa = 10.1EE28 pKa = 4.07LIRR31 pKa = 11.84RR32 pKa = 11.84QNGKK36 pKa = 9.97CPITGRR42 pKa = 11.84DD43 pKa = 3.79LRR45 pKa = 11.84SMTSTNVVVDD55 pKa = 4.18HH56 pKa = 6.37DD57 pKa = 4.44HH58 pKa = 6.44KK59 pKa = 11.31SGVIRR64 pKa = 11.84AALPRR69 pKa = 11.84AINGLEE75 pKa = 3.97GKK77 pKa = 8.2LTNLCIRR84 pKa = 11.84WGGCTSKK91 pKa = 10.9RR92 pKa = 11.84DD93 pKa = 4.02IINMLRR99 pKa = 11.84GMADD103 pKa = 3.46YY104 pKa = 11.31LEE106 pKa = 4.38LHH108 pKa = 5.9QVPQTEE114 pKa = 4.78WIHH117 pKa = 6.23PEE119 pKa = 3.72HH120 pKa = 6.5LTPAEE125 pKa = 3.84ARR127 pKa = 11.84AKK129 pKa = 10.41RR130 pKa = 11.84NAKK133 pKa = 9.14ARR135 pKa = 11.84KK136 pKa = 9.25RR137 pKa = 11.84YY138 pKa = 9.41ALKK141 pKa = 10.79KK142 pKa = 9.56KK143 pKa = 10.69DD144 pKa = 3.37KK145 pKa = 10.92

Molecular weight: 16.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30

0

30

12585

61

1229

419.5

47.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.24 ± 0.499

0.985 ± 0.161

6.667 ± 0.246

7.66 ± 0.407

3.949 ± 0.195

7.31 ± 0.446

1.859 ± 0.215

5.427 ± 0.223

6.643 ± 0.39

8.176 ± 0.316

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.209

4.974 ± 0.215

3.639 ± 0.193

4.346 ± 0.269

5.189 ± 0.268

5.729 ± 0.359

5.356 ± 0.342

6.126 ± 0.345

1.375 ± 0.148

3.576 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski