Syntrophomonas zehnderi OL-4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Syntrophomonadaceae; Syntrophomonas; Syntrophomonas zehnderi

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2631 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3W367|A0A0E3W367_9FIRM Phosphoribulokinase/uridine kinase OS=Syntrophomonas zehnderi OL-4 OX=690567 GN=1414 PE=4 SV=1
MM1 pKa = 7.59LRR3 pKa = 11.84DD4 pKa = 3.5LSEE7 pKa = 4.25KK8 pKa = 10.47VSYY11 pKa = 10.43LQGLSEE17 pKa = 4.41GLNITEE23 pKa = 5.08GNPQGKK29 pKa = 9.34IISGILGVLNEE40 pKa = 4.13MSEE43 pKa = 4.74EE44 pKa = 3.84ISQLQEE50 pKa = 3.89DD51 pKa = 3.75MDD53 pKa = 4.64EE54 pKa = 3.85IRR56 pKa = 11.84EE57 pKa = 3.96YY58 pKa = 11.35LEE60 pKa = 5.87NIDD63 pKa = 6.16DD64 pKa = 4.79DD65 pKa = 5.27LLDD68 pKa = 3.93LEE70 pKa = 4.49EE71 pKa = 5.35TIFEE75 pKa = 4.83DD76 pKa = 4.04NEE78 pKa = 3.87FMEE81 pKa = 4.93ISCRR85 pKa = 11.84NCGEE89 pKa = 4.03KK90 pKa = 10.75LFIEE94 pKa = 4.37SDD96 pKa = 2.92IFADD100 pKa = 3.66VEE102 pKa = 4.15DD103 pKa = 5.01SIEE106 pKa = 4.5VICPCCNEE114 pKa = 4.05VVFVNDD120 pKa = 2.95NCFDD124 pKa = 4.31CGHH127 pKa = 6.93EE128 pKa = 4.32SPEE131 pKa = 4.25LNADD135 pKa = 3.56TSRR138 pKa = 11.84GYY140 pKa = 10.84LL141 pKa = 3.36

Molecular weight: 15.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E4G9V7|A0A0E4G9V7_9FIRM Crotonase superfamily OS=Syntrophomonas zehnderi OL-4 OX=690567 GN=594 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPKK8 pKa = 8.36NRR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.01KK14 pKa = 7.87VHH16 pKa = 5.71GFRR19 pKa = 11.84KK20 pKa = 9.93RR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.19VLAMRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.71KK37 pKa = 10.47GRR39 pKa = 11.84KK40 pKa = 8.85SLSAA44 pKa = 3.86

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2631

0

2631

801858

20

2180

304.8

33.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.068 ± 0.052

1.203 ± 0.022

5.401 ± 0.032

6.763 ± 0.058

3.765 ± 0.033

7.25 ± 0.053

1.766 ± 0.02

7.743 ± 0.04

6.002 ± 0.038

9.934 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.795 ± 0.026

4.5 ± 0.036

4.011 ± 0.029

3.901 ± 0.029

4.852 ± 0.037

5.664 ± 0.037

5.057 ± 0.053

6.808 ± 0.033

0.961 ± 0.018

3.54 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski