Methylovorus sp. MM2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Methylophilaceae; Methylovorus; unclassified Methylovorus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2256 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A9T466|A0A1A9T466_9PROT Uncharacterized protein OS=Methylovorus sp. MM2 OX=1848038 GN=A7981_04980 PE=4 SV=1
MM1 pKa = 7.45PTFISNFLSSDD12 pKa = 4.27AIRR15 pKa = 11.84EE16 pKa = 4.18AFGGIDD22 pKa = 3.4AVDD25 pKa = 3.61EE26 pKa = 4.19QMEE29 pKa = 4.61SLGEE33 pKa = 4.04ALDD36 pKa = 3.64IAFSDD41 pKa = 4.56EE42 pKa = 4.33YY43 pKa = 10.98LGPAYY48 pKa = 9.62EE49 pKa = 4.43VVPSSILASNNAMSGRR65 pKa = 11.84LVNGDD70 pKa = 3.68SFNITGKK77 pKa = 10.2EE78 pKa = 3.71LLADD82 pKa = 3.71YY83 pKa = 9.54PHH85 pKa = 7.47IYY87 pKa = 10.11SFIYY91 pKa = 10.13RR92 pKa = 11.84FHH94 pKa = 7.07QGGSVSFSGDD104 pKa = 2.61MSYY107 pKa = 9.21YY108 pKa = 10.15TNPNNLPGYY117 pKa = 9.64EE118 pKa = 4.07NTFDD122 pKa = 4.16GYY124 pKa = 10.21VSKK127 pKa = 11.16LIIEE131 pKa = 4.63SGQGSKK137 pKa = 7.49VTYY140 pKa = 9.66IGRR143 pKa = 11.84GNIEE147 pKa = 4.32SNGDD151 pKa = 3.11INEE154 pKa = 4.24FVATSMQLQIGKK166 pKa = 10.19LSLVVNGEE174 pKa = 4.09INLSFVDD181 pKa = 5.76DD182 pKa = 4.1IDD184 pKa = 4.21DD185 pKa = 3.38VSGIVTGFNLTSGKK199 pKa = 10.57YY200 pKa = 8.37SFSMSGLVLDD210 pKa = 5.37AVDD213 pKa = 4.54FDD215 pKa = 5.06AYY217 pKa = 10.91SEE219 pKa = 4.42SGFDD223 pKa = 4.78FLDD226 pKa = 3.65NLLAGNDD233 pKa = 3.81TLDD236 pKa = 3.96GNVSNNMIYY245 pKa = 10.91GLVGDD250 pKa = 4.95DD251 pKa = 5.06SINGLSGNDD260 pKa = 3.01ILYY263 pKa = 10.66GGEE266 pKa = 4.17GSDD269 pKa = 3.62TLVGGIGNDD278 pKa = 3.56TLDD281 pKa = 4.49GGDD284 pKa = 4.23GDD286 pKa = 4.59DD287 pKa = 4.45SLVGGAGNDD296 pKa = 3.5VYY298 pKa = 11.0IVGEE302 pKa = 3.94FGDD305 pKa = 4.44IVDD308 pKa = 4.0EE309 pKa = 4.77TISGSSGLDD318 pKa = 2.98RR319 pKa = 11.84VKK321 pKa = 10.89LGSTYY326 pKa = 11.62DD327 pKa = 3.33MTHH330 pKa = 6.62YY331 pKa = 11.2DD332 pKa = 3.19LTDD335 pKa = 3.26NVEE338 pKa = 4.25NLDD341 pKa = 3.63ASAVTTVWPSTWLEE355 pKa = 3.97LYY357 pKa = 10.69GNSLANVIIGSSSNDD372 pKa = 3.22YY373 pKa = 11.24LIGLDD378 pKa = 4.91GNDD381 pKa = 3.77TLIGGAGDD389 pKa = 5.17DD390 pKa = 4.06ILLGGAGVDD399 pKa = 3.7NLQGGAGNDD408 pKa = 3.65TYY410 pKa = 11.75NVNLKK415 pKa = 9.64TIGTGVNITAALEE428 pKa = 3.97DD429 pKa = 4.94TITDD433 pKa = 3.7TAGTADD439 pKa = 3.85TLVLLGGLGANKK451 pKa = 9.63TSTITLVAALEE462 pKa = 4.13NLDD465 pKa = 3.85ASSAEE470 pKa = 4.06VNNTIVGAVALNLTGNASNNEE491 pKa = 4.17IIGSNGKK498 pKa = 7.99NTILGLAGNDD508 pKa = 4.08TIDD511 pKa = 4.62GLDD514 pKa = 3.95GDD516 pKa = 4.93DD517 pKa = 5.18SIDD520 pKa = 3.55GGVGNDD526 pKa = 3.75SLSGGDD532 pKa = 3.8GDD534 pKa = 4.04DD535 pKa = 3.87TYY537 pKa = 12.11VVALKK542 pKa = 10.56LQGSGAGANVIIEE555 pKa = 4.17DD556 pKa = 4.61TIDD559 pKa = 3.25EE560 pKa = 4.55ALDD563 pKa = 3.46EE564 pKa = 4.74GRR566 pKa = 11.84DD567 pKa = 3.75TLRR570 pKa = 11.84LAGSAKK576 pKa = 9.05LTNASTIEE584 pKa = 4.03LTGDD588 pKa = 3.88LANIEE593 pKa = 4.79NIDD596 pKa = 3.58ASLTGSTLLNLIGNGSSNYY615 pKa = 10.43LIGNSAANWLDD626 pKa = 3.6GGDD629 pKa = 4.59GDD631 pKa = 5.08DD632 pKa = 4.22TLDD635 pKa = 3.89GGGGANTMIGGFGNDD650 pKa = 2.8TYY652 pKa = 11.73VVNNAKK658 pKa = 10.45DD659 pKa = 3.66VVFDD663 pKa = 3.84GVNPSILRR671 pKa = 11.84LSIDD675 pKa = 3.54SQGNEE680 pKa = 3.71ANTGGFNAIYY690 pKa = 9.86SINDD694 pKa = 3.27RR695 pKa = 11.84YY696 pKa = 11.18VIFVSGASNLVSNDD710 pKa = 3.2NNLSADD716 pKa = 3.7VFIKK720 pKa = 10.47DD721 pKa = 3.33IQTGNVTRR729 pKa = 11.84VSTNSADD736 pKa = 3.46QEE738 pKa = 4.77SNHH741 pKa = 5.91VYY743 pKa = 10.67DD744 pKa = 5.27AVPEE748 pKa = 4.07LAISLDD754 pKa = 3.1GKK756 pKa = 10.43YY757 pKa = 10.57LVFSTTDD764 pKa = 2.97TGLVNGDD771 pKa = 3.53VNDD774 pKa = 3.77TRR776 pKa = 11.84DD777 pKa = 3.24IYY779 pKa = 11.35LKK781 pKa = 10.52NLQTGEE787 pKa = 4.01IKK789 pKa = 10.84LVDD792 pKa = 3.58VSINGAHH799 pKa = 6.43TDD801 pKa = 3.13SHH803 pKa = 7.85GYY805 pKa = 10.48EE806 pKa = 4.08SFNPSFSPDD815 pKa = 3.19SKK817 pKa = 11.34SVLFSSFDD825 pKa = 3.61PLLVNNGNASNVGVYY840 pKa = 9.82IKK842 pKa = 10.52NIQTGAVKK850 pKa = 10.36LVSTNSLGDD859 pKa = 3.62SANDD863 pKa = 3.04ATYY866 pKa = 10.36AYY868 pKa = 9.99GYY870 pKa = 10.67SSDD873 pKa = 3.39GKK875 pKa = 10.75KK876 pKa = 9.8ILLVSSANNFTVGDD890 pKa = 3.97SNGFQDD896 pKa = 4.97VFIKK900 pKa = 10.64NLQTNAVQLINTDD913 pKa = 3.32KK914 pKa = 11.39NGNQANNHH922 pKa = 5.84SYY924 pKa = 11.4NPLFSKK930 pKa = 10.63DD931 pKa = 2.89GNYY934 pKa = 9.69VVFEE938 pKa = 4.26SLASNLVEE946 pKa = 4.8EE947 pKa = 4.44YY948 pKa = 10.39TDD950 pKa = 3.58YY951 pKa = 11.67GKK953 pKa = 10.67LQIYY957 pKa = 9.62IKK959 pKa = 10.46NLQTGDD965 pKa = 3.21IQLVSKK971 pKa = 11.33NEE973 pKa = 4.05LGEE976 pKa = 4.2AGYY979 pKa = 11.15GDD981 pKa = 4.52SLNASISSNGRR992 pKa = 11.84YY993 pKa = 7.99VVFMSSASNLVVGDD1007 pKa = 3.91TNGMLDD1013 pKa = 3.38VFVRR1017 pKa = 11.84DD1018 pKa = 4.12LYY1020 pKa = 9.43TNEE1023 pKa = 3.83VKK1025 pKa = 10.59RR1026 pKa = 11.84ISVSLNGQEE1035 pKa = 4.64GNGDD1039 pKa = 3.86SYY1041 pKa = 11.83TNSALLGGSTFSSDD1055 pKa = 2.42GGYY1058 pKa = 10.12ILFHH1062 pKa = 6.91SNASNLDD1069 pKa = 3.3ASDD1072 pKa = 3.95NNSVADD1078 pKa = 3.72VFRR1081 pKa = 11.84VANPFLIADD1090 pKa = 5.45GIDD1093 pKa = 3.14IVIAAISYY1101 pKa = 8.21TLGADD1106 pKa = 3.43IEE1108 pKa = 4.3NLTLSGTSGLSGRR1121 pKa = 11.84GNDD1124 pKa = 3.96LDD1126 pKa = 4.54NVITGNSGANKK1137 pKa = 9.68LQSLVGNDD1145 pKa = 3.6TLLGGAGSDD1154 pKa = 3.72TLDD1157 pKa = 3.68GGLGIDD1163 pKa = 4.22SMEE1166 pKa = 4.08GSAGNDD1172 pKa = 3.21VYY1174 pKa = 11.02IVDD1177 pKa = 5.6DD1178 pKa = 4.04INDD1181 pKa = 4.09LVVEE1185 pKa = 4.23TDD1187 pKa = 3.57VNSKK1191 pKa = 10.6SGGIDD1196 pKa = 2.96TVRR1199 pKa = 11.84IANTFIGAYY1208 pKa = 8.5YY1209 pKa = 10.9ALIANVEE1216 pKa = 4.12NLDD1219 pKa = 3.33GSAYY1223 pKa = 9.77ISGIDD1228 pKa = 3.44LRR1230 pKa = 11.84GNGLVNTITGGIGNDD1245 pKa = 3.51TLAGSAYY1252 pKa = 8.75EE1253 pKa = 3.87TDD1255 pKa = 3.37QYY1257 pKa = 11.66INDD1260 pKa = 3.99GLVDD1264 pKa = 3.61TFKK1267 pKa = 11.04GGKK1270 pKa = 9.95GDD1272 pKa = 3.64DD1273 pKa = 3.87TYY1275 pKa = 11.69HH1276 pKa = 6.76VFLQTTGSANKK1287 pKa = 8.89ATVSIQDD1294 pKa = 4.26SIVEE1298 pKa = 4.29SANQGTDD1305 pKa = 2.95TVILHH1310 pKa = 6.7GSDD1313 pKa = 3.45NNTLTKK1319 pKa = 10.38ASKK1322 pKa = 8.69ITLTANLEE1330 pKa = 4.15HH1331 pKa = 7.3LDD1333 pKa = 4.52ASDD1336 pKa = 5.08TDD1338 pKa = 4.42STWLNLTGNVASNQITGNDD1357 pKa = 3.4GHH1359 pKa = 7.29NIILGLGGNDD1369 pKa = 2.87ILYY1372 pKa = 10.47GGNGHH1377 pKa = 6.84DD1378 pKa = 4.31TLDD1381 pKa = 3.69GGIGNDD1387 pKa = 3.72VLNGGADD1394 pKa = 3.75DD1395 pKa = 4.95DD1396 pKa = 4.49KK1397 pKa = 11.78LIGGLGNDD1405 pKa = 3.97TLTGGNGSDD1414 pKa = 2.5IFLFNTALNATTNVDD1429 pKa = 3.82TITDD1433 pKa = 4.13FTSGEE1438 pKa = 3.86DD1439 pKa = 4.23HH1440 pKa = 7.3LYY1442 pKa = 10.7LDD1444 pKa = 3.51NAIFKK1449 pKa = 10.78KK1450 pKa = 9.5LTNEE1454 pKa = 4.19SALSADD1460 pKa = 3.08NFYY1463 pKa = 11.48VVGSGAQTAHH1473 pKa = 5.85QFIIYY1478 pKa = 10.22DD1479 pKa = 3.79GASGNLFYY1487 pKa = 11.13DD1488 pKa = 3.72ADD1490 pKa = 4.25GSGLLAPVLFANLEE1504 pKa = 4.14SAPALTAGDD1513 pKa = 3.72IQIVV1517 pKa = 3.37

Molecular weight: 157.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A9T200|A0A1A9T200_9PROT Uncharacterized protein OS=Methylovorus sp. MM2 OX=1848038 GN=A7981_00760 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 8.87VRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.65GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84ARR41 pKa = 11.84LAVV44 pKa = 3.42

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2256

0

2256

731518

39

3267

324.3

35.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.725 ± 0.058

0.78 ± 0.018

5.6 ± 0.048

5.793 ± 0.058

3.822 ± 0.037

7.297 ± 0.08

2.211 ± 0.031

6.597 ± 0.038

5.148 ± 0.055

10.368 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.498 ± 0.029

4.072 ± 0.06

4.233 ± 0.038

4.031 ± 0.041

4.902 ± 0.05

6.326 ± 0.046

5.555 ± 0.074

6.978 ± 0.048

1.18 ± 0.023

2.886 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski