Wenzhou tombus-like virus 15

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KGT9|A0A1L3KGT9_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 15 OX=1923668 PE=4 SV=1
MM1 pKa = 7.29GKK3 pKa = 9.34IVAKK7 pKa = 9.97VMRR10 pKa = 11.84TYY12 pKa = 10.05TYY14 pKa = 11.05HH15 pKa = 7.74LIKK18 pKa = 9.98QTDD21 pKa = 3.22QGAVTKK27 pKa = 11.01YY28 pKa = 8.46MDD30 pKa = 3.83EE31 pKa = 4.36NAPHH35 pKa = 6.72FPFNVKK41 pKa = 9.8KK42 pKa = 9.73YY43 pKa = 10.55QPILTDD49 pKa = 3.59PEE51 pKa = 4.41VTNVDD56 pKa = 3.61YY57 pKa = 11.56ADD59 pKa = 4.83LLFLDD64 pKa = 3.63MRR66 pKa = 11.84EE67 pKa = 3.75NFARR71 pKa = 11.84EE72 pKa = 3.86KK73 pKa = 10.56TRR75 pKa = 11.84VQTTKK80 pKa = 10.43PEE82 pKa = 4.23HH83 pKa = 5.33KK84 pKa = 8.6TVRR87 pKa = 11.84SAVSNLYY94 pKa = 8.72GTDD97 pKa = 3.5TPNSEE102 pKa = 3.8QHH104 pKa = 6.0RR105 pKa = 11.84QFKK108 pKa = 10.71SKK110 pKa = 10.82ARR112 pKa = 11.84GGKK115 pKa = 9.18SKK117 pKa = 10.42GRR119 pKa = 11.84SRR121 pKa = 11.84TFCKK125 pKa = 8.31TTQQQHH131 pKa = 5.41NNTYY135 pKa = 10.0NKK137 pKa = 10.01QNNRR141 pKa = 11.84LQNVPQTSTRR151 pKa = 11.84GLPTINAIVNDD162 pKa = 3.93IIDD165 pKa = 3.46RR166 pKa = 11.84HH167 pKa = 5.0TRR169 pKa = 11.84PMSRR173 pKa = 11.84SPVSSGRR180 pKa = 11.84TTPSSTATATSVDD193 pKa = 4.0TPKK196 pKa = 10.02STTSSIKK203 pKa = 9.18STYY206 pKa = 7.39TTGTDD211 pKa = 3.48PVPTSNRR218 pKa = 11.84FIYY221 pKa = 10.51LDD223 pKa = 3.42YY224 pKa = 11.37DD225 pKa = 3.54NNEE228 pKa = 4.56NISEE232 pKa = 4.27DD233 pKa = 3.03EE234 pKa = 3.75RR235 pKa = 11.84AYY237 pKa = 11.12YY238 pKa = 7.86GTRR241 pKa = 11.84AIDD244 pKa = 3.51RR245 pKa = 11.84CDD247 pKa = 3.37AACGTDD253 pKa = 3.24GLLIEE258 pKa = 5.71AEE260 pKa = 4.2IQTEE264 pKa = 4.05EE265 pKa = 4.77DD266 pKa = 3.62DD267 pKa = 5.84LMIAGIKK274 pKa = 10.26AKK276 pKa = 9.99EE277 pKa = 4.16KK278 pKa = 8.01PAKK281 pKa = 9.28YY282 pKa = 9.83QSLHH286 pKa = 6.46GDD288 pKa = 3.06IEE290 pKa = 4.2IRR292 pKa = 11.84DD293 pKa = 4.12FKK295 pKa = 11.33QDD297 pKa = 2.84IPYY300 pKa = 10.5KK301 pKa = 10.81LNALDD306 pKa = 3.52NHH308 pKa = 6.21EE309 pKa = 5.16CFSQFKK315 pKa = 9.9NDD317 pKa = 3.44KK318 pKa = 9.96LGSDD322 pKa = 5.2FIFEE326 pKa = 4.16PLYY329 pKa = 10.25TYY331 pKa = 10.66LVRR334 pKa = 11.84HH335 pKa = 6.12SCAKK339 pKa = 10.1YY340 pKa = 8.92ATRR343 pKa = 11.84DD344 pKa = 3.67DD345 pKa = 5.58KK346 pKa = 11.07IAHH349 pKa = 5.0YY350 pKa = 10.43NKK352 pKa = 10.21LRR354 pKa = 11.84MRR356 pKa = 11.84FINEE360 pKa = 3.88HH361 pKa = 6.52LNHH364 pKa = 7.05KK365 pKa = 9.82DD366 pKa = 3.43YY367 pKa = 11.47KK368 pKa = 10.37FLRR371 pKa = 11.84EE372 pKa = 3.84PTKK375 pKa = 9.74QQEE378 pKa = 4.35ILLHH382 pKa = 5.68HH383 pKa = 6.0TVQRR387 pKa = 11.84CADD390 pKa = 3.75NNEE393 pKa = 4.18LDD395 pKa = 3.91WIYY398 pKa = 11.14EE399 pKa = 4.16STKK402 pKa = 10.11PPRR405 pKa = 11.84EE406 pKa = 3.75GSLRR410 pKa = 11.84SIWRR414 pKa = 11.84SVRR417 pKa = 11.84RR418 pKa = 11.84IPTRR422 pKa = 11.84ITRR425 pKa = 11.84VFTSTIAEE433 pKa = 4.18SGAVEE438 pKa = 4.2NNVTSTIEE446 pKa = 4.16NIPKK450 pKa = 8.05YY451 pKa = 9.76CYY453 pKa = 9.43WSGSHH458 pKa = 6.33PTQEE462 pKa = 5.38DD463 pKa = 3.69NTNHH467 pKa = 5.52QLKK470 pKa = 9.54EE471 pKa = 3.58HH472 pKa = 6.88AIWKK476 pKa = 9.7EE477 pKa = 3.6GDD479 pKa = 3.55VQPSKK484 pKa = 11.1DD485 pKa = 3.52KK486 pKa = 11.22SPCEE490 pKa = 3.8VKK492 pKa = 10.14HH493 pKa = 5.12YY494 pKa = 11.12KK495 pKa = 10.44RR496 pKa = 11.84MFNIPGYY503 pKa = 9.68DD504 pKa = 3.66DD505 pKa = 4.67KK506 pKa = 11.45PVVMNNCKK514 pKa = 10.35CNEE517 pKa = 3.7VEE519 pKa = 4.07ALHH522 pKa = 6.62NRR524 pKa = 11.84YY525 pKa = 9.94LKK527 pKa = 10.06DD528 pKa = 2.98TGFYY532 pKa = 10.58QPDD535 pKa = 3.97KK536 pKa = 10.49IDD538 pKa = 3.12WDD540 pKa = 4.08IIDD543 pKa = 4.81GLVQVLAGKK552 pKa = 9.32LLKK555 pKa = 10.89LSDD558 pKa = 3.94SKK560 pKa = 11.72NPAGLLEE567 pKa = 4.86PNTVEE572 pKa = 4.06EE573 pKa = 4.91FISEE577 pKa = 4.0KK578 pKa = 10.79SGALKK583 pKa = 10.49NRR585 pKa = 11.84YY586 pKa = 8.97CEE588 pKa = 3.82AATNLIANGVDD599 pKa = 3.61KK600 pKa = 11.52NIGTVDD606 pKa = 3.4AFIKK610 pKa = 9.93NEE612 pKa = 4.04KK613 pKa = 9.52YY614 pKa = 10.71SEE616 pKa = 4.08IKK618 pKa = 9.16TPRR621 pKa = 11.84AIMGRR626 pKa = 11.84NPQFNLLYY634 pKa = 10.24QRR636 pKa = 11.84YY637 pKa = 6.59ITKK640 pKa = 10.34AEE642 pKa = 4.17KK643 pKa = 10.92LFMQLPQVAKK653 pKa = 10.02GKK655 pKa = 10.16NFIDD659 pKa = 3.95RR660 pKa = 11.84GKK662 pKa = 10.23QFSKK666 pKa = 10.83ILGDD670 pKa = 3.96WILEE674 pKa = 3.79NDD676 pKa = 3.28YY677 pKa = 11.57SKK679 pKa = 11.53FEE681 pKa = 4.08GSQRR685 pKa = 11.84TPVLMRR691 pKa = 11.84IEE693 pKa = 3.94YY694 pKa = 10.48RR695 pKa = 11.84LLCLLYY701 pKa = 10.58GQSEE705 pKa = 4.5DD706 pKa = 4.78LDD708 pKa = 4.47NIYY711 pKa = 8.19QTKK714 pKa = 10.11LYY716 pKa = 10.45KK717 pKa = 10.12KK718 pKa = 10.14GRR720 pKa = 11.84FPEE723 pKa = 4.4GTKK726 pKa = 10.22FCFKK730 pKa = 10.31GCRR733 pKa = 11.84GSGDD737 pKa = 3.42MDD739 pKa = 3.48TGFGNTVLNYY749 pKa = 9.88IATQYY754 pKa = 10.62FRR756 pKa = 11.84LKK758 pKa = 8.52NAKK761 pKa = 9.75YY762 pKa = 9.14VTDD765 pKa = 3.52KK766 pKa = 11.54DD767 pKa = 3.78KK768 pKa = 11.27FVVDD772 pKa = 4.61GDD774 pKa = 4.69DD775 pKa = 5.49SYY777 pKa = 12.0QQLDD781 pKa = 3.5KK782 pKa = 11.46GVTANKK788 pKa = 9.85LVNTYY793 pKa = 10.22EE794 pKa = 4.03YY795 pKa = 10.87FGFEE799 pKa = 4.15TKK801 pKa = 10.83LMIRR805 pKa = 11.84KK806 pKa = 9.23DD807 pKa = 3.33PHH809 pKa = 7.7DD810 pKa = 3.96VEE812 pKa = 4.89FCSSKK817 pKa = 10.32FLEE820 pKa = 4.53YY821 pKa = 10.68KK822 pKa = 9.82PGEE825 pKa = 4.13YY826 pKa = 10.22VQALSLKK833 pKa = 10.25KK834 pKa = 10.39LLNNIQYY841 pKa = 10.93LNQTQHH847 pKa = 5.14EE848 pKa = 4.44HH849 pKa = 5.92SADD852 pKa = 3.17VYY854 pKa = 9.51YY855 pKa = 11.16ASLGIMYY862 pKa = 9.96RR863 pKa = 11.84RR864 pKa = 11.84IYY866 pKa = 10.86GDD868 pKa = 3.09IPIYY872 pKa = 10.65SHH874 pKa = 6.51LAEE877 pKa = 4.35YY878 pKa = 11.18LMTASGQYY886 pKa = 9.82SYY888 pKa = 11.67EE889 pKa = 4.29LIRR892 pKa = 11.84TVNASYY898 pKa = 10.54IDD900 pKa = 3.58HH901 pKa = 6.73VKK903 pKa = 10.8DD904 pKa = 3.74LNGDD908 pKa = 4.41LKK910 pKa = 10.98CDD912 pKa = 3.59QSLTKK917 pKa = 10.65AGITLCFGISQEE929 pKa = 4.09EE930 pKa = 4.25QINLIRR936 pKa = 11.84FFQNNTLSLPEE947 pKa = 4.52EE948 pKa = 4.14YY949 pKa = 9.96CHH951 pKa = 7.77RR952 pKa = 11.84YY953 pKa = 8.94RR954 pKa = 11.84APKK957 pKa = 10.19NKK959 pKa = 9.86YY960 pKa = 8.82KK961 pKa = 10.9LEE963 pKa = 4.62NIPLSLIDD971 pKa = 4.87SMGCVKK977 pKa = 10.32PVIQTQGGPIPIIPEE992 pKa = 3.31FDD994 pKa = 3.75MIIEE998 pKa = 4.37ATTTNAPPIVNAEE1011 pKa = 4.37KK1012 pKa = 10.15IDD1014 pKa = 3.72AALKK1018 pKa = 10.17NRR1020 pKa = 11.84SITKK1024 pKa = 10.3RR1025 pKa = 11.84IRR1027 pKa = 11.84HH1028 pKa = 5.4LFKK1031 pKa = 10.66RR1032 pKa = 11.84SQPSSGDD1039 pKa = 3.2GNVEE1043 pKa = 3.83INEE1046 pKa = 3.86VDD1048 pKa = 3.04

Molecular weight:
120.25 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KGT9|A0A1L3KGT9_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 15 OX=1923668 PE=4 SV=1
MM1 pKa = 7.48SKK3 pKa = 10.29QYY5 pKa = 11.17VPVTYY10 pKa = 10.45SRR12 pKa = 11.84PVKK15 pKa = 10.59SKK17 pKa = 9.85AATIKK22 pKa = 10.65RR23 pKa = 11.84SGTSILVSHH32 pKa = 6.68EE33 pKa = 4.47EE34 pKa = 4.17YY35 pKa = 10.49IQDD38 pKa = 3.73VVATTAFSCITIPINPGVQTSFPWLSLLAAGYY70 pKa = 9.41EE71 pKa = 4.35SYY73 pKa = 10.63SIKK76 pKa = 10.83SLVVEE81 pKa = 4.21YY82 pKa = 10.38EE83 pKa = 4.04PRR85 pKa = 11.84CATTTQGTVILAFDD99 pKa = 4.43SDD101 pKa = 4.38ASDD104 pKa = 3.68PAPPGKK110 pKa = 9.63VQAMQYY116 pKa = 10.03DD117 pKa = 4.3PNIAIPPWQRR127 pKa = 11.84EE128 pKa = 3.92GLRR131 pKa = 11.84FKK133 pKa = 11.17PEE135 pKa = 3.25IQKK138 pKa = 10.09ISRR141 pKa = 11.84HH142 pKa = 4.37FVRR145 pKa = 11.84FGNLAANQDD154 pKa = 3.02IKK156 pKa = 11.36LYY158 pKa = 10.22DD159 pKa = 3.5VCNLFLITQGFTSAGIQVGEE179 pKa = 3.54IHH181 pKa = 6.33FRR183 pKa = 11.84YY184 pKa = 10.09VIEE187 pKa = 4.45FQTPQIDD194 pKa = 3.76YY195 pKa = 10.88DD196 pKa = 4.29GYY198 pKa = 11.67ALQTSARR205 pKa = 11.84ISVTPADD212 pKa = 3.38QVLFTTGTVIAGGAPITINTGPLTLAKK239 pKa = 10.19VAGGGSSAATLTTSNQAMVFGGPGQYY265 pKa = 10.38YY266 pKa = 9.61FNVLIGSTGGITITAANVGIYY287 pKa = 9.65TLGTVSLIYY296 pKa = 10.64FGTPGNASYY305 pKa = 11.73VNYY308 pKa = 10.91NFICKK313 pKa = 9.77VNSPGDD319 pKa = 4.04AIALVTGATSTTWTSAGSSIRR340 pKa = 11.84VSYY343 pKa = 9.79YY344 pKa = 10.87AYY346 pKa = 10.77ALNN349 pKa = 3.84

Molecular weight:
37.27 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1397

349

1048

698.5

78.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.228 ± 1.789

1.646 ± 0.255

5.727 ± 1.311

5.154 ± 1.311

3.865 ± 0.22

6.156 ± 1.388

2.076 ± 0.619

7.301 ± 0.659

7.015 ± 1.967

7.015 ± 0.508

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.503 ± 0.328

5.87 ± 0.946

4.868 ± 0.439

4.581 ± 0.148

4.796 ± 0.983

6.729 ± 0.951

8.017 ± 1.17

5.369 ± 1.205

0.644 ± 0.11

5.44 ± 0.148

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski