Betacoronavirus Erinaceus/VMC/DEU/2012

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Merbecovirus; Hedgehog coronavirus 1

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5LMN3|U5LMN3_9BETC Orf5 OS=Betacoronavirus Erinaceus/VMC/DEU/2012 OX=1385427 GN=orf5 PE=4 SV=1
MM1 pKa = 6.7QSKK4 pKa = 10.5RR5 pKa = 11.84GILTLIVCNILAMSLAKK22 pKa = 10.44HH23 pKa = 6.69FIPEE27 pKa = 3.56HH28 pKa = 5.26CANYY32 pKa = 9.38EE33 pKa = 3.68GAMFHH38 pKa = 7.09ACVQNAISTAAGTYY52 pKa = 8.98TNTKK56 pKa = 9.65LSYY59 pKa = 10.29PVFDD63 pKa = 4.2NDD65 pKa = 3.6GVTYY69 pKa = 11.05YY70 pKa = 10.96DD71 pKa = 3.3ATQDD75 pKa = 3.27RR76 pKa = 11.84DD77 pKa = 3.51ATPSYY82 pKa = 9.78EE83 pKa = 3.66HH84 pKa = 7.15SEE86 pKa = 4.33FYY88 pKa = 10.94EE89 pKa = 4.25LDD91 pKa = 3.5EE92 pKa = 5.16SSFSAKK98 pKa = 9.6PVLAEE103 pKa = 4.03LQQ105 pKa = 3.46

Molecular weight: 11.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5LR11|U5LR11_9BETC 3C-like proteinase OS=Betacoronavirus Erinaceus/VMC/DEU/2012 OX=1385427 GN=ORF 1a PE=3 SV=1
MM1 pKa = 7.64ATPQQPRR8 pKa = 11.84AVTFADD14 pKa = 3.85NNGNQQNGNNRR25 pKa = 11.84GRR27 pKa = 11.84PRR29 pKa = 11.84QPKK32 pKa = 8.87PRR34 pKa = 11.84PAPNVSVSWYY44 pKa = 9.74TGITQHH50 pKa = 6.06GKK52 pKa = 9.7QPLAFPAGQGVPLNANSTPKK72 pKa = 10.38QNAGYY77 pKa = 9.38WRR79 pKa = 11.84RR80 pKa = 11.84QDD82 pKa = 3.65RR83 pKa = 11.84KK84 pKa = 10.35LNTGNGVKK92 pKa = 9.99QLAPRR97 pKa = 11.84WYY99 pKa = 9.84FYY101 pKa = 10.45YY102 pKa = 10.42TGTGPEE108 pKa = 3.91ANLPFRR114 pKa = 11.84TVKK117 pKa = 10.79DD118 pKa = 3.7GIYY121 pKa = 9.43WVWEE125 pKa = 4.34EE126 pKa = 4.44GASEE130 pKa = 4.17APSDD134 pKa = 3.72FGTRR138 pKa = 11.84NPANDD143 pKa = 3.63AAIVTQFAPGTQLPKK158 pKa = 9.78NCHH161 pKa = 5.54IEE163 pKa = 4.21GTGGNSQSSSRR174 pKa = 11.84ASSASRR180 pKa = 11.84NSSRR184 pKa = 11.84SNSRR188 pKa = 11.84GSQPGSRR195 pKa = 11.84SHH197 pKa = 6.83SPGSVGPSDD206 pKa = 4.68ASALLYY212 pKa = 11.02LEE214 pKa = 4.08LAKK217 pKa = 10.7RR218 pKa = 11.84LEE220 pKa = 3.99ALEE223 pKa = 4.35AGKK226 pKa = 10.58SKK228 pKa = 10.59SAPKK232 pKa = 10.61VITKK236 pKa = 10.2KK237 pKa = 10.55DD238 pKa = 3.07AADD241 pKa = 3.38AKK243 pKa = 10.87KK244 pKa = 10.6KK245 pKa = 9.11MRR247 pKa = 11.84HH248 pKa = 5.25KK249 pKa = 10.55RR250 pKa = 11.84VATKK254 pKa = 10.31AYY256 pKa = 10.64NPTQAFGLRR265 pKa = 11.84GPGDD269 pKa = 3.51LQGNFGDD276 pKa = 5.16LKK278 pKa = 9.27YY279 pKa = 10.76CKK281 pKa = 9.97EE282 pKa = 4.28GVDD285 pKa = 4.07DD286 pKa = 4.43PRR288 pKa = 11.84WPQMAEE294 pKa = 3.91LAPSASAFLSMSQLKK309 pKa = 9.78LVHH312 pKa = 6.65HH313 pKa = 7.09SNDD316 pKa = 3.31TDD318 pKa = 3.53NKK320 pKa = 7.67PVYY323 pKa = 8.78MLRR326 pKa = 11.84YY327 pKa = 9.45SGAIKK332 pKa = 10.61LDD334 pKa = 3.7PKK336 pKa = 10.55NPNYY340 pKa = 10.73SKK342 pKa = 10.29WLEE345 pKa = 3.92ILEE348 pKa = 4.42GNIDD352 pKa = 3.55AYY354 pKa = 10.34KK355 pKa = 10.71NFPKK359 pKa = 10.26KK360 pKa = 9.87EE361 pKa = 4.02KK362 pKa = 9.93KK363 pKa = 9.84QKK365 pKa = 9.97QSQIKK370 pKa = 9.79QEE372 pKa = 4.21AEE374 pKa = 3.8DD375 pKa = 4.7PEE377 pKa = 4.57WDD379 pKa = 3.7STDD382 pKa = 4.88LFSQPLQDD390 pKa = 5.14PDD392 pKa = 3.92NQPKK396 pKa = 9.44AQRR399 pKa = 11.84VPKK402 pKa = 10.63GSITQRR408 pKa = 11.84SRR410 pKa = 11.84PPKK413 pKa = 7.77PTVGPMVDD421 pKa = 3.43VEE423 pKa = 4.21PP424 pKa = 4.76

Molecular weight: 46.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

14639

81

7150

1219.9

135.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.022 ± 0.198

3.224 ± 0.254

5.103 ± 0.374

4.317 ± 0.303

4.871 ± 0.18

5.718 ± 0.444

2.008 ± 0.156

4.509 ± 0.357

5.929 ± 0.453

9.584 ± 0.521

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.527 ± 0.214

5.595 ± 0.252

3.907 ± 0.58

3.696 ± 0.495

3.156 ± 0.255

7.576 ± 0.398

5.991 ± 0.282

9.488 ± 0.807

1.141 ± 0.12

4.638 ± 0.284
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski