Aspergillus aculeatus (strain ATCC 16872 / CBS 172.66 / WB 5094)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Circumdati; Aspergillus aculeatus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10842 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L9X954|A0A1L9X954_ASPA1 GMC_OxRdtase_N domain-containing protein OS=Aspergillus aculeatus (strain ATCC 16872 / CBS 172.66 / WB 5094) OX=690307 GN=ASPACDRAFT_74505 PE=3 SV=1
MM1 pKa = 7.43QIAHH5 pKa = 7.53LLALSGLMLATTVTAGGDD23 pKa = 3.53LDD25 pKa = 5.59RR26 pKa = 11.84NDD28 pKa = 3.84VPNTCWEE35 pKa = 3.9PCGPVVNISHH45 pKa = 6.96ACDD48 pKa = 3.18AQTDD52 pKa = 3.63NDD54 pKa = 4.2RR55 pKa = 11.84AEE57 pKa = 4.17LQCVCGWDD65 pKa = 3.47RR66 pKa = 11.84AATLIPLCEE75 pKa = 3.86ACIGYY80 pKa = 9.77YY81 pKa = 8.64RR82 pKa = 11.84TQQHH86 pKa = 6.89HH87 pKa = 7.06DD88 pKa = 3.62HH89 pKa = 7.13DD90 pKa = 5.77HH91 pKa = 7.79DD92 pKa = 6.21DD93 pKa = 4.99DD94 pKa = 5.59HH95 pKa = 9.08DD96 pKa = 4.49HH97 pKa = 7.92DD98 pKa = 5.15NDD100 pKa = 4.78NDD102 pKa = 4.87DD103 pKa = 6.17DD104 pKa = 6.9DD105 pKa = 7.55DD106 pKa = 7.68DD107 pKa = 7.61DD108 pKa = 7.65DD109 pKa = 7.65DD110 pKa = 7.65DD111 pKa = 7.65DD112 pKa = 7.65DD113 pKa = 7.69DD114 pKa = 7.65DD115 pKa = 7.64DD116 pKa = 7.38DD117 pKa = 6.7DD118 pKa = 7.16DD119 pKa = 6.4NDD121 pKa = 3.79RR122 pKa = 11.84HH123 pKa = 6.82DD124 pKa = 3.99NNAYY128 pKa = 10.3DD129 pKa = 4.15ILTSCSFSTTTWNPAAATSVLSAVHH154 pKa = 7.12ASTTGNGNATASTSASATGAGAAGTTTAAAGTGGGRR190 pKa = 11.84DD191 pKa = 3.43SVSTTDD197 pKa = 3.48GSSGATGRR205 pKa = 11.84ADD207 pKa = 3.1SGTATGAVSAEE218 pKa = 4.09GTRR221 pKa = 11.84VTDD224 pKa = 3.38NAAPGLVAGRR234 pKa = 11.84EE235 pKa = 4.12AASFAAVVGVGVLALLL251 pKa = 4.28

Molecular weight: 25.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L9WPT2|A0A1L9WPT2_ASPA1 Carboxylic ester hydrolase OS=Aspergillus aculeatus (strain ATCC 16872 / CBS 172.66 / WB 5094) OX=690307 GN=ASPACDRAFT_1904121 PE=3 SV=1
MM1 pKa = 6.58THH3 pKa = 6.26EE4 pKa = 4.66SVWYY8 pKa = 9.72SRR10 pKa = 11.84PRR12 pKa = 11.84QFGKK16 pKa = 10.48GSRR19 pKa = 11.84GCRR22 pKa = 11.84VCTHH26 pKa = 6.9RR27 pKa = 11.84AGLIRR32 pKa = 11.84KK33 pKa = 8.95YY34 pKa = 11.19GMNICRR40 pKa = 11.84QCFRR44 pKa = 11.84EE45 pKa = 4.08KK46 pKa = 10.78ASDD49 pKa = 2.76IGFYY53 pKa = 10.54KK54 pKa = 10.53FRR56 pKa = 5.74

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10842

0

10842

5397569

49

7329

497.8

55.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.998 ± 0.02

1.266 ± 0.009

5.533 ± 0.015

6.076 ± 0.024

3.682 ± 0.014

6.857 ± 0.022

2.518 ± 0.012

4.76 ± 0.015

4.177 ± 0.019

9.283 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.092 ± 0.008

3.42 ± 0.012

6.037 ± 0.023

4.113 ± 0.016

6.248 ± 0.022

8.128 ± 0.03

6.143 ± 0.017

6.313 ± 0.017

1.506 ± 0.009

2.851 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski