Parcubacteria bacterium RAAC4_OD1_1

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Patescibacteria group; Parcubacteria group; Candidatus Parcubacteria; unclassified Parcubacteria

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 686 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V7PVL6|V7PVL6_9BACT 50S ribosomal protein L32 OS=Parcubacteria bacterium RAAC4_OD1_1 OX=1394712 GN=rpmF PE=3 SV=1
MM1 pKa = 7.33IKK3 pKa = 10.26KK4 pKa = 9.57IVSLVVMTSFVLTMTGVFPLTLMAASISSSKK35 pKa = 10.01ATFGRR40 pKa = 11.84LKK42 pKa = 10.74ASTPSDD48 pKa = 3.41SVIIQFVSPTGIQTGGADD66 pKa = 3.94TISLTFSSDD75 pKa = 3.24FTVAPEE81 pKa = 3.69AVANFDD87 pKa = 3.61VGLGNSGVCSSATYY101 pKa = 9.22TDD103 pKa = 4.4EE104 pKa = 5.36IISLTASATDD114 pKa = 3.11WGVDD118 pKa = 3.18VTGNVITFSPEE129 pKa = 3.39TDD131 pKa = 3.48DD132 pKa = 4.72VLTAGYY138 pKa = 9.57CIRR141 pKa = 11.84IEE143 pKa = 4.17MGTAATTGGAGSASTITNGLADD165 pKa = 5.43DD166 pKa = 5.78DD167 pKa = 4.26DD168 pKa = 5.14TITIAGGFGDD178 pKa = 4.25TGVMTVDD185 pKa = 4.63IVDD188 pKa = 4.43DD189 pKa = 4.21DD190 pKa = 4.23QVTVTATVNQTMTFDD205 pKa = 4.76LDD207 pKa = 3.72TSVTDD212 pKa = 3.89SEE214 pKa = 4.23TDD216 pKa = 3.06APYY219 pKa = 9.75TVPLGVLSSSSVKK232 pKa = 10.52VSGSTDD238 pKa = 3.26SVNMIIAEE246 pKa = 4.29GNTNASGGMNVTVKK260 pKa = 10.44NANGANGLVSTSTPADD276 pKa = 4.41RR277 pKa = 11.84IEE279 pKa = 4.37SATGTMIAGTEE290 pKa = 3.95NYY292 pKa = 9.05GLCVASASLAGFTRR306 pKa = 11.84ATAYY310 pKa = 7.7NTNCALNSEE319 pKa = 4.52TNAIIGLTTTTADD332 pKa = 4.44LLTSSAPISSAHH344 pKa = 6.52AEE346 pKa = 4.07IVVNAAIAGATPAHH360 pKa = 6.48TDD362 pKa = 3.33YY363 pKa = 11.4TDD365 pKa = 3.11TLTFIATASYY375 pKa = 11.15

Molecular weight: 37.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V7PZN2|V7PZN2_9BACT Uncharacterized protein OS=Parcubacteria bacterium RAAC4_OD1_1 OX=1394712 GN=O210_OD1C00001G0610 PE=4 SV=1
MM1 pKa = 7.07SQTYY5 pKa = 9.4QPNKK9 pKa = 9.02RR10 pKa = 11.84KK11 pKa = 9.56RR12 pKa = 11.84AKK14 pKa = 8.9THH16 pKa = 5.79GFLSRR21 pKa = 11.84TKK23 pKa = 9.21TPGGRR28 pKa = 11.84KK29 pKa = 6.09VTQARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.76GRR39 pKa = 11.84VRR41 pKa = 11.84VSIKK45 pKa = 10.36KK46 pKa = 10.0

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

686

0

686

206949

29

3710

301.7

34.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.944 ± 0.1

0.773 ± 0.043

5.515 ± 0.064

7.41 ± 0.144

4.832 ± 0.082

6.736 ± 0.13

1.439 ± 0.039

9.88 ± 0.133

9.543 ± 0.192

8.646 ± 0.105

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.042

6.224 ± 0.095

3.221 ± 0.048

2.217 ± 0.042

3.436 ± 0.069

6.995 ± 0.108

5.272 ± 0.204

6.015 ± 0.07

0.899 ± 0.032

3.766 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski