Phasey bean mild yellows virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus; unclassified Polerovirus

Average proteome isoelectric point is 8.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S3JNY5|A0A0S3JNY5_9LUTE Movement protein OS=Phasey bean mild yellows virus OX=1756832 GN=MP PE=3 SV=1
MM1 pKa = 7.53NSFSVLFFFFCAFFCVAPVISGPGPVQPEE30 pKa = 3.76WAASKK35 pKa = 10.05AANSMWPDD43 pKa = 3.88LQPGWGFTSPCPPCPVASCQFLLAQSSLDD72 pKa = 3.7VEE74 pKa = 4.69QTSAPPTYY82 pKa = 10.08KK83 pKa = 10.82GLILDD88 pKa = 4.3LWGLASSDD96 pKa = 3.18AKK98 pKa = 10.37TSFSAACLSLQNSWASTAVFLSRR121 pKa = 11.84GFEE124 pKa = 3.78TAFRR128 pKa = 11.84HH129 pKa = 5.38LCWLAIYY136 pKa = 9.7VWYY139 pKa = 10.67SLLRR143 pKa = 11.84SIIVTSARR151 pKa = 11.84FILNNSILALAGTSLFVCTALLVKK175 pKa = 9.99LVRR178 pKa = 11.84WIFGLMPIYY187 pKa = 10.6LVFTFPISVGRR198 pKa = 11.84WICQKK203 pKa = 10.44RR204 pKa = 11.84FWGKK208 pKa = 9.38FSRR211 pKa = 11.84NYY213 pKa = 10.79AEE215 pKa = 5.03EE216 pKa = 4.29KK217 pKa = 10.24CCEE220 pKa = 3.95QFLSFTINQDD230 pKa = 3.45PPKK233 pKa = 10.09KK234 pKa = 9.83CQLVFQKK241 pKa = 10.82EE242 pKa = 4.32DD243 pKa = 3.48GKK245 pKa = 10.22HH246 pKa = 5.82AGYY249 pKa = 7.72GTCVRR254 pKa = 11.84LFNGTNGLLTAYY266 pKa = 9.62HH267 pKa = 5.92VASNSSKK274 pKa = 10.36VVSTRR279 pKa = 11.84TGNKK283 pKa = 9.29IPLSQFKK290 pKa = 9.91PLIVSPLYY298 pKa = 10.84DD299 pKa = 3.24QVLYY303 pKa = 10.84AGPTEE308 pKa = 4.19WEE310 pKa = 4.19SLLGCKK316 pKa = 9.49GVNFAPAKK324 pKa = 9.46TLGASKK330 pKa = 10.75CNIYY334 pKa = 10.22HH335 pKa = 5.6IQKK338 pKa = 9.42NGKK341 pKa = 7.99WGCTNAEE348 pKa = 3.74IEE350 pKa = 4.46GQLKK354 pKa = 9.79HH355 pKa = 6.95CEE357 pKa = 3.85GNRR360 pKa = 11.84XXKK363 pKa = 9.93HH364 pKa = 6.74CEE366 pKa = 3.58GNRR369 pKa = 11.84DD370 pKa = 3.71VLPGQLSVLSNTEE383 pKa = 4.01PGQSGAGYY391 pKa = 10.32FNGKK395 pKa = 6.52TLVAIHH401 pKa = 6.09VGGSLEE407 pKa = 5.14RR408 pKa = 11.84EE409 pKa = 4.34DD410 pKa = 4.69SCATYY415 pKa = 10.84NVAVPVLPKK424 pKa = 10.38PGLTSPHH431 pKa = 5.96YY432 pKa = 10.56VFEE435 pKa = 4.55TTAPTSGVYY444 pKa = 9.8DD445 pKa = 3.6SKK447 pKa = 11.34IFTALDD453 pKa = 3.49EE454 pKa = 4.57AVEE457 pKa = 4.01QAEE460 pKa = 4.2RR461 pKa = 11.84WVKK464 pKa = 9.62MKK466 pKa = 10.67LSKK469 pKa = 11.43GEE471 pKa = 4.17TLWADD476 pKa = 3.95LEE478 pKa = 4.93DD479 pKa = 4.42DD480 pKa = 4.15LPYY483 pKa = 10.7EE484 pKa = 4.25VEE486 pKa = 4.18TNKK489 pKa = 8.97TAKK492 pKa = 10.23APPSSSSPTEE502 pKa = 3.61TSLDD506 pKa = 3.76CEE508 pKa = 4.23QSGKK512 pKa = 10.03RR513 pKa = 11.84DD514 pKa = 3.08ARR516 pKa = 11.84LRR518 pKa = 11.84PRR520 pKa = 11.84NNRR523 pKa = 11.84KK524 pKa = 9.66DD525 pKa = 3.38RR526 pKa = 11.84IKK528 pKa = 10.22PRR530 pKa = 11.84KK531 pKa = 9.02RR532 pKa = 11.84GLRR535 pKa = 11.84HH536 pKa = 5.17PSKK539 pKa = 10.65RR540 pKa = 11.84YY541 pKa = 10.14ADD543 pKa = 3.06WGTNNEE549 pKa = 4.35DD550 pKa = 3.09VGGEE554 pKa = 3.99NRR556 pKa = 11.84FQYY559 pKa = 10.09QPEE562 pKa = 3.87RR563 pKa = 11.84SRR565 pKa = 11.84EE566 pKa = 4.17GFCGSDD572 pKa = 2.79QGTGPEE578 pKa = 4.01EE579 pKa = 4.19TKK581 pKa = 10.76PVITPAAKK589 pKa = 9.71QEE591 pKa = 4.33RR592 pKa = 11.84RR593 pKa = 11.84QQWIAKK599 pKa = 6.23TNNYY603 pKa = 9.47RR604 pKa = 11.84RR605 pKa = 11.84FFDD608 pKa = 3.93SQFNWEE614 pKa = 4.29VCPSEE619 pKa = 4.17QEE621 pKa = 3.92EE622 pKa = 4.42VAGFRR627 pKa = 11.84FCGKK631 pKa = 10.08APQWYY636 pKa = 9.17HH637 pKa = 6.45PKK639 pKa = 10.11QKK641 pKa = 10.42QEE643 pKa = 4.15GGWGEE648 pKa = 4.3EE649 pKa = 4.2VCRR652 pKa = 11.84XHH654 pKa = 7.74PEE656 pKa = 3.94MGEE659 pKa = 4.07KK660 pKa = 8.78THH662 pKa = 6.72GFGWPQFGPQAEE674 pKa = 4.44LKK676 pKa = 10.49SLRR679 pKa = 11.84LQAARR684 pKa = 11.84WLQRR688 pKa = 11.84AQSAEE693 pKa = 4.15VPPLPEE699 pKa = 3.77RR700 pKa = 11.84EE701 pKa = 3.84RR702 pKa = 11.84VIKK705 pKa = 10.55RR706 pKa = 11.84LVSAYY711 pKa = 9.64KK712 pKa = 9.77QAQSINPVATMSGEE726 pKa = 4.18LLWEE730 pKa = 4.65DD731 pKa = 3.57FLEE734 pKa = 4.39SFKK737 pKa = 11.06EE738 pKa = 4.23AVSSLQLDD746 pKa = 3.63AGVGVPYY753 pKa = 10.48VALGKK758 pKa = 7.54PTHH761 pKa = 6.73RR762 pKa = 11.84SLVEE766 pKa = 4.24DD767 pKa = 4.17PEE769 pKa = 4.25MLPVLARR776 pKa = 11.84LTFDD780 pKa = 3.9RR781 pKa = 11.84LEE783 pKa = 4.28KK784 pKa = 10.39LSKK787 pKa = 11.34GEE789 pKa = 3.81ICRR792 pKa = 11.84LTPEE796 pKa = 3.87EE797 pKa = 4.25LVRR800 pKa = 11.84EE801 pKa = 5.51GYY803 pKa = 10.29CDD805 pKa = 4.76PIRR808 pKa = 11.84VFVKK812 pKa = 10.78GEE814 pKa = 3.59PHH816 pKa = 6.19KK817 pKa = 10.54QSKK820 pKa = 10.21LDD822 pKa = 3.38EE823 pKa = 4.27GRR825 pKa = 11.84YY826 pKa = 8.96RR827 pKa = 11.84LIMSVSLVDD836 pKa = 3.45QLVARR841 pKa = 11.84VLFQEE846 pKa = 4.22QNKK849 pKa = 9.84KK850 pKa = 10.25EE851 pKa = 3.95IQLWRR856 pKa = 11.84VVPSKK861 pKa = 10.72PGFGLSTDD869 pKa = 3.72GQVLEE874 pKa = 4.38FTEE877 pKa = 4.52ALAHH881 pKa = 6.1KK882 pKa = 10.54VGVTPQDD889 pKa = 4.47LIEE892 pKa = 4.2NWKK895 pKa = 10.36QYY897 pKa = 10.81LVPTDD902 pKa = 3.64CSGFDD907 pKa = 3.29WSVADD912 pKa = 4.67WMLDD916 pKa = 3.24DD917 pKa = 6.35DD918 pKa = 4.21IEE920 pKa = 4.34VRR922 pKa = 11.84NRR924 pKa = 11.84LTRR927 pKa = 11.84GLTPVTALLRR937 pKa = 11.84RR938 pKa = 11.84NWKK941 pKa = 8.96HH942 pKa = 5.45CXANSVLCLSDD953 pKa = 3.3GTLLAQCVPGVQKK966 pKa = 10.73SGSYY970 pKa = 8.25NTSSSNSRR978 pKa = 11.84IRR980 pKa = 11.84VMSAYY985 pKa = 10.18HH986 pKa = 7.12CGATWCCAMGDD997 pKa = 3.94DD998 pKa = 4.78ALEE1001 pKa = 4.44SVDD1004 pKa = 5.31SNLEE1008 pKa = 3.79EE1009 pKa = 4.26YY1010 pKa = 10.37KK1011 pKa = 10.82RR1012 pKa = 11.84LGLKK1016 pKa = 10.26VEE1018 pKa = 4.21VSGKK1022 pKa = 10.76LEE1024 pKa = 4.05FCSHH1028 pKa = 6.17IFEE1031 pKa = 4.85SPSLATPVNVGKK1043 pKa = 9.09MLYY1046 pKa = 10.29KK1047 pKa = 10.69LIYY1050 pKa = 9.53GYY1052 pKa = 11.16NPGCEE1057 pKa = 4.03NLEE1060 pKa = 3.91VTANYY1065 pKa = 9.75LAACFSVFNEE1075 pKa = 3.97LRR1077 pKa = 11.84HH1078 pKa = 6.13DD1079 pKa = 4.07PEE1081 pKa = 4.41LVRR1084 pKa = 11.84VLYY1087 pKa = 9.74EE1088 pKa = 3.87WLVLPVQSQNNTT1100 pKa = 3.13

Molecular weight: 122.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7T1C3P2|A0A7T1C3P2_9LUTE P3-P5 fusion protein OS=Phasey bean mild yellows virus OX=1756832 GN=RTD PE=4 SV=1
MM1 pKa = 6.44NTVVARR7 pKa = 11.84NNGMGRR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 9.59RR16 pKa = 11.84RR17 pKa = 11.84NRR19 pKa = 11.84RR20 pKa = 11.84VTRR23 pKa = 11.84RR24 pKa = 11.84QRR26 pKa = 11.84VVVVQAPGLPRR37 pKa = 11.84RR38 pKa = 11.84GRR40 pKa = 11.84RR41 pKa = 11.84QRR43 pKa = 11.84RR44 pKa = 11.84NRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84ASRR51 pKa = 11.84GGRR54 pKa = 11.84AGGGRR59 pKa = 11.84SSEE62 pKa = 4.23TFVLSKK68 pKa = 11.29DD69 pKa = 3.47NLAGSSSGSITFGPSLSEE87 pKa = 3.84KK88 pKa = 10.46PEE90 pKa = 3.95FSSGILKK97 pKa = 10.28AYY99 pKa = 10.25HH100 pKa = 6.63EE101 pKa = 4.49YY102 pKa = 10.72KK103 pKa = 10.29IXXXXLEE110 pKa = 5.38FISEE114 pKa = 4.26ASXTSSGSIAYY125 pKa = 9.43EE126 pKa = 3.79LDD128 pKa = 3.31PHH130 pKa = 6.88CKK132 pKa = 9.85YY133 pKa = 11.04SSLQSSINKK142 pKa = 9.84FGITKK147 pKa = 10.16NGSRR151 pKa = 11.84SWSSKK156 pKa = 9.9FINGEE161 pKa = 3.83EE162 pKa = 3.87WHH164 pKa = 7.19DD165 pKa = 3.55ASEE168 pKa = 4.12DD169 pKa = 3.52QFRR172 pKa = 11.84ILYY175 pKa = 8.98KK176 pKa = 11.06GNGASSIAGSFRR188 pKa = 11.84ITFKK192 pKa = 11.13CQFQNPKK199 pKa = 10.32

Molecular weight: 21.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3144

45

1100

449.1

49.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.997 ± 0.459

2.449 ± 0.497

3.658 ± 0.54

5.98 ± 0.54

4.23 ± 0.474

7.729 ± 0.372

1.527 ± 0.207

3.658 ± 0.392

5.503 ± 0.764

8.524 ± 1.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.24 ± 0.139

4.676 ± 0.376

6.266 ± 0.523

3.658 ± 0.403

6.743 ± 1.161

9.733 ± 0.79

5.057 ± 0.432

6.266 ± 0.516

2.036 ± 0.372

2.99 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski