Halomonas urumqiensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3488 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N7UI90|A0A2N7UI90_9GAMM 50S ribosomal protein L30 OS=Halomonas urumqiensis OX=1684789 GN=rpmD PE=3 SV=1
MM1 pKa = 7.17VKK3 pKa = 10.14PMRR6 pKa = 11.84GGGMSSRR13 pKa = 11.84GDD15 pKa = 3.24QRR17 pKa = 11.84GAALVVTLVLLVVALMLGLSSFQSARR43 pKa = 11.84LEE45 pKa = 3.75EE46 pKa = 4.51SMAGNQRR53 pKa = 11.84ASVQALLAAEE63 pKa = 4.23YY64 pKa = 9.82GASKK68 pKa = 10.6AWLEE72 pKa = 4.13FDD74 pKa = 3.87PSSLSSEE81 pKa = 4.15EE82 pKa = 4.16GVLVSDD88 pKa = 5.38DD89 pKa = 4.0RR90 pKa = 11.84DD91 pKa = 3.36NVYY94 pKa = 10.86YY95 pKa = 10.15SWSVTPDD102 pKa = 2.96GYY104 pKa = 11.59GKK106 pKa = 10.69YY107 pKa = 10.18LFNSVGEE114 pKa = 4.23VMAGSDD120 pKa = 4.64LISQRR125 pKa = 11.84VISYY129 pKa = 10.48LGILSIGPGGTIVAANNDD147 pKa = 3.92DD148 pKa = 3.83DD149 pKa = 5.41CEE151 pKa = 4.16EE152 pKa = 4.61GSVEE156 pKa = 4.32FVPPSSNMEE165 pKa = 3.86VTGEE169 pKa = 4.11EE170 pKa = 4.16EE171 pKa = 4.17VNGNIKK177 pKa = 10.49AAVQVGCGIIAEE189 pKa = 4.86NIASSIIPKK198 pKa = 10.31NGEE201 pKa = 3.29FDD203 pKa = 4.17DD204 pKa = 4.85YY205 pKa = 12.0VMHH208 pKa = 7.27DD209 pKa = 4.21TGDD212 pKa = 3.56DD213 pKa = 3.69TYY215 pKa = 11.23TCLDD219 pKa = 3.26GGGNNRR225 pKa = 11.84LCNYY229 pKa = 9.87YY230 pKa = 10.75GGIQGGIDD238 pKa = 4.5LDD240 pKa = 3.67ILKK243 pKa = 10.26NADD246 pKa = 3.11MLAQFVYY253 pKa = 10.32GIRR256 pKa = 11.84DD257 pKa = 3.53NYY259 pKa = 10.73SEE261 pKa = 5.18SIVNSIPSSVPEE273 pKa = 3.99GSITYY278 pKa = 9.68VKK280 pKa = 10.61SADD283 pKa = 3.66DD284 pKa = 3.65ADD286 pKa = 4.23GNRR289 pKa = 11.84EE290 pKa = 3.93TFSRR294 pKa = 11.84SGNFTGSGILIVDD307 pKa = 4.01GNVDD311 pKa = 4.17FGGVPGFEE319 pKa = 3.96GLVIVLGDD327 pKa = 3.65YY328 pKa = 10.18VVSGGGGGEE337 pKa = 4.07FKK339 pKa = 10.91GSVVSAPIVDD349 pKa = 4.13SACWDD354 pKa = 3.26AGAATGCEE362 pKa = 3.91FDD364 pKa = 4.69DD365 pKa = 4.55KK366 pKa = 11.02KK367 pKa = 11.17IEE369 pKa = 4.0IGGGGSSSYY378 pKa = 11.13SYY380 pKa = 11.5DD381 pKa = 3.11PLAILNAFEE390 pKa = 5.76LIDD393 pKa = 4.47GSGLDD398 pKa = 4.15EE399 pKa = 4.64LWGVDD404 pKa = 3.3NQTLKK409 pKa = 11.29GNFYY413 pKa = 8.59MASWSEE419 pKa = 4.18LIGNYY424 pKa = 9.62

Molecular weight: 44.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N7UK81|A0A2N7UK81_9GAMM 1-deoxy-D-xylulose-5-phosphate synthase OS=Halomonas urumqiensis OX=1684789 GN=dxs PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3488

0

3488

1180462

37

7991

338.4

37.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.274 ± 0.053

0.903 ± 0.013

5.919 ± 0.043

6.489 ± 0.044

3.424 ± 0.027

8.433 ± 0.045

2.504 ± 0.025

4.707 ± 0.033

2.46 ± 0.037

11.459 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.569 ± 0.025

2.518 ± 0.026

4.931 ± 0.033

3.737 ± 0.028

7.223 ± 0.048

5.489 ± 0.03

4.993 ± 0.036

7.31 ± 0.035

1.446 ± 0.018

2.213 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski