Acaryochloris phage A-HIS2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X8XE39|A0A0X8XE39_9CAUD Virion structural protein OS=Acaryochloris phage A-HIS2 OX=645117 GN=AHIS2_p069 PE=4 SV=1
MM1 pKa = 7.38LTQTQIATLHH11 pKa = 4.93NTGVFSEE18 pKa = 4.98EE19 pKa = 3.89IYY21 pKa = 9.75EE22 pKa = 4.29TFADD26 pKa = 3.76DD27 pKa = 4.31AGITFEE33 pKa = 4.92CILWTSQRR41 pKa = 11.84IYY43 pKa = 11.12GDD45 pKa = 3.95EE46 pKa = 4.07SLMDD50 pKa = 5.06PDD52 pKa = 4.37NMGAFDD58 pKa = 3.87QVVEE62 pKa = 4.19DD63 pKa = 5.42FEE65 pKa = 6.52DD66 pKa = 4.12KK67 pKa = 10.76FDD69 pKa = 3.92PQLFEE74 pKa = 4.22RR75 pKa = 11.84VCMILEE81 pKa = 4.3CAA83 pKa = 4.25

Molecular weight: 9.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A120N0D2|A0A120N0D2_9CAUD Putative virion structural protein OS=Acaryochloris phage A-HIS2 OX=645117 GN=AHIS2_p101 PE=4 SV=1
MM1 pKa = 7.29QLYY4 pKa = 9.21TSVNQFSRR12 pKa = 11.84SLHH15 pKa = 5.98LNFLRR20 pKa = 11.84FRR22 pKa = 11.84LGWHH26 pKa = 6.01VSPSRR31 pKa = 11.84DD32 pKa = 3.28YY33 pKa = 9.59FTRR36 pKa = 11.84SPKK39 pKa = 9.92VDD41 pKa = 3.22LRR43 pKa = 11.84EE44 pKa = 3.98DD45 pKa = 3.38RR46 pKa = 11.84GRR48 pKa = 11.84HH49 pKa = 4.52IFTLAFLSSHH59 pKa = 6.45VMLIDD64 pKa = 3.38NVNFEE69 pKa = 4.22GSYY72 pKa = 11.25GSLEE76 pKa = 4.13RR77 pKa = 11.84LGQMWNIRR85 pKa = 11.84VLYY88 pKa = 10.36RR89 pKa = 11.84SDD91 pKa = 3.17KK92 pKa = 11.02

Molecular weight: 11.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

17540

38

1431

168.7

18.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.235 ± 0.508

1.254 ± 0.179

6.363 ± 0.212

6.482 ± 0.349

4.276 ± 0.232

7.161 ± 0.49

1.881 ± 0.236

5.667 ± 0.177

4.966 ± 0.44

9.498 ± 0.335

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.235 ± 0.217

4.846 ± 0.213

4.19 ± 0.28

3.883 ± 0.251

5.029 ± 0.258

6.551 ± 0.329

6.995 ± 0.454

6.397 ± 0.17

1.408 ± 0.164

3.683 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski