bacterium HR19

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1704 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5XST9|A0A2H5XST9_9BACT Single-stranded-DNA-specific exonuclease RecJ OS=bacterium HR19 OX=2035414 GN=recJ PE=3 SV=1
MM1 pKa = 7.87PSGFTSTSKK10 pKa = 10.66PLDD13 pKa = 4.28CNDD16 pKa = 3.84SNASIYY22 pKa = 10.42PGAPLNCNNSQDD34 pKa = 3.76NDD36 pKa = 3.5CDD38 pKa = 4.31GNVEE42 pKa = 3.8KK43 pKa = 10.46WFFQDD48 pKa = 3.66QDD50 pKa = 3.36SDD52 pKa = 3.4TWTTSISQCANTMPSGFTSTSKK74 pKa = 10.66PLDD77 pKa = 4.28CNDD80 pKa = 3.84SNASIYY86 pKa = 10.42PGAPLNCNNSQDD98 pKa = 3.76NDD100 pKa = 3.5CDD102 pKa = 4.31GNVEE106 pKa = 3.8KK107 pKa = 10.46WFFQDD112 pKa = 3.66QDD114 pKa = 3.36SDD116 pKa = 3.4TWTTSISQCANTMPSGFTSTSKK138 pKa = 10.66PLDD141 pKa = 4.28CNDD144 pKa = 3.84SNASIYY150 pKa = 10.42PGAPLNCNNSQDD162 pKa = 3.76NDD164 pKa = 3.5CDD166 pKa = 4.31GNVEE170 pKa = 3.8KK171 pKa = 10.46WFFQDD176 pKa = 3.66QDD178 pKa = 3.36SDD180 pKa = 3.42TWTTSVSQCANTMPSGFTSTSKK202 pKa = 10.68PLDD205 pKa = 4.16CNDD208 pKa = 4.15FSPLINPGQPEE219 pKa = 4.03ICNGVDD225 pKa = 3.61DD226 pKa = 4.8NCDD229 pKa = 3.23GVIDD233 pKa = 4.7DD234 pKa = 4.54VACGVPVCPTSLTATYY250 pKa = 9.27FFRR253 pKa = 11.84SSTGDD258 pKa = 3.06ISIKK262 pKa = 10.8LEE264 pKa = 4.11WNDD267 pKa = 3.23PATNEE272 pKa = 3.96TGFVVEE278 pKa = 4.65KK279 pKa = 10.31SKK281 pKa = 11.02KK282 pKa = 9.49SSFFPSITYY291 pKa = 9.33VINSPNTTTFTLDD304 pKa = 3.19FEE306 pKa = 4.79EE307 pKa = 4.78PLTRR311 pKa = 11.84FWFRR315 pKa = 11.84VWAFNSTVPCTPIAISSVFTPPGLLWAYY343 pKa = 10.2SLTSIARR350 pKa = 11.84DD351 pKa = 3.36QNNYY355 pKa = 9.09PIHH358 pKa = 6.69PKK360 pKa = 10.14IFDD363 pKa = 3.95LNGDD367 pKa = 3.82GKK369 pKa = 11.03KK370 pKa = 9.93EE371 pKa = 4.05VIMSDD376 pKa = 2.62SGGRR380 pKa = 11.84VYY382 pKa = 10.81AISTTEE388 pKa = 3.7FNLEE392 pKa = 3.48ISAIPLWIIRR402 pKa = 11.84PAGNKK407 pKa = 9.75KK408 pKa = 8.58FTTNSIAVFQGNATVIAINQQDD430 pKa = 3.64SRR432 pKa = 11.84SYY434 pKa = 10.8IIDD437 pKa = 3.25KK438 pKa = 10.54NGNVTKK444 pKa = 10.78NIALGTTPPYY454 pKa = 10.17HH455 pKa = 7.34AVITDD460 pKa = 3.27INFDD464 pKa = 4.41LLPDD468 pKa = 5.22FIVSLQNQVKK478 pKa = 10.52AFDD481 pKa = 3.92FFGNNLFTADD491 pKa = 3.74PPGAEE496 pKa = 4.41VIGTPTALIEE506 pKa = 4.33YY507 pKa = 9.28KK508 pKa = 10.78GKK510 pKa = 6.91TTTYY514 pKa = 10.66VVGTNTRR521 pKa = 11.84KK522 pKa = 9.39IRR524 pKa = 11.84FYY526 pKa = 10.81EE527 pKa = 4.24GNNQISVIDD536 pKa = 3.85LTLQPFSGGVPSFISTFKK554 pKa = 10.74KK555 pKa = 10.18GALSRR560 pKa = 11.84AVVGTDD566 pKa = 2.78TDD568 pKa = 3.49IFITNLIKK576 pKa = 11.06NPLLTFNTNAQVIATPLFFDD596 pKa = 4.68FSGDD600 pKa = 3.5GITDD604 pKa = 3.45VAITSGNFLRR614 pKa = 11.84IINGATGLEE623 pKa = 3.75ICSFNLGANTDD634 pKa = 3.73SSPVLLPTTPVQILVGANNGILYY657 pKa = 10.01SISPSCTQNWQYY669 pKa = 11.55NAGLGFQIKK678 pKa = 8.4STPLSANLDD687 pKa = 3.97SNSSIEE693 pKa = 4.34IIFTATSATSGKK705 pKa = 9.92IVILDD710 pKa = 3.46SSGNLIKK717 pKa = 10.67EE718 pKa = 3.99FSDD721 pKa = 3.75LVNIGPTVSSPRR733 pKa = 11.84AGDD736 pKa = 3.13IDD738 pKa = 4.43GNGVTDD744 pKa = 3.66VVIGGSKK751 pKa = 10.59VIGTQGGIYY760 pKa = 9.95IVTCGTDD767 pKa = 3.2CSSANPKK774 pKa = 10.12KK775 pKa = 10.27FSKK778 pKa = 10.7SGWSEE783 pKa = 3.65IKK785 pKa = 7.75TTPLLYY791 pKa = 10.61DD792 pKa = 4.4LNSDD796 pKa = 3.97GYY798 pKa = 11.28LDD800 pKa = 4.14IVVADD805 pKa = 4.34TGNNMYY811 pKa = 10.26AISGTSINYY820 pKa = 9.58SEE822 pKa = 5.93PYY824 pKa = 10.72LLWQDD829 pKa = 3.37NLTKK833 pKa = 10.14IGEE836 pKa = 4.38TPPGVVFPSSPTVFLKK852 pKa = 11.16GSIPYY857 pKa = 9.57IAIGSNGQGIYY868 pKa = 10.09IIRR871 pKa = 11.84GNDD874 pKa = 2.99GSAQNIGICNKK885 pKa = 8.8TFSSPATFDD894 pKa = 3.48YY895 pKa = 11.13NRR897 pKa = 11.84DD898 pKa = 3.58GIADD902 pKa = 4.03LVFGCDD908 pKa = 2.85NKK910 pKa = 10.35FVYY913 pKa = 10.0IISGSDD919 pKa = 3.09FSVLRR924 pKa = 11.84KK925 pKa = 9.78EE926 pKa = 4.14KK927 pKa = 10.95VGDD930 pKa = 3.75KK931 pKa = 10.85VRR933 pKa = 11.84GSPKK937 pKa = 9.93IYY939 pKa = 10.6DD940 pKa = 3.43FDD942 pKa = 6.29GDD944 pKa = 4.12GVQDD948 pKa = 3.58IGIGSDD954 pKa = 3.38DD955 pKa = 3.64KK956 pKa = 11.53NIYY959 pKa = 8.93VFNGEE964 pKa = 4.03RR965 pKa = 11.84TFICSFPTPVQNQPAIFRR983 pKa = 11.84DD984 pKa = 3.8KK985 pKa = 10.78IIFSDD990 pKa = 3.7SSNLYY995 pKa = 9.71VLNSDD1000 pKa = 3.13NCIAVAQKK1008 pKa = 10.94NGVSLSPSSSCGVFYY1023 pKa = 9.12EE1024 pKa = 4.37TAGGKK1029 pKa = 9.9EE1030 pKa = 3.57ILICGDD1036 pKa = 3.43EE1037 pKa = 4.46TNGEE1041 pKa = 4.25ILVMDD1046 pKa = 4.62SALNDD1051 pKa = 3.18IFPFPFTFASATFFSSPAIDD1071 pKa = 5.97DD1072 pKa = 3.54IDD1074 pKa = 4.9GDD1076 pKa = 3.95SSLEE1080 pKa = 3.93FVISSQDD1087 pKa = 3.0MVFAYY1092 pKa = 10.53RR1093 pKa = 11.84LTNSEE1098 pKa = 4.81FEE1100 pKa = 4.3RR1101 pKa = 11.84LWGEE1105 pKa = 4.39FSHH1108 pKa = 7.36DD1109 pKa = 3.61RR1110 pKa = 11.84YY1111 pKa = 10.9NSSFKK1116 pKa = 9.52EE1117 pKa = 4.01QKK1119 pKa = 9.28EE1120 pKa = 4.14VSYY1123 pKa = 10.54LSPNISVSKK1132 pKa = 10.56IKK1134 pKa = 10.97VPDD1137 pKa = 3.47LHH1139 pKa = 7.13EE1140 pKa = 4.79SEE1142 pKa = 5.46SKK1144 pKa = 10.88GCSTSMPYY1152 pKa = 10.29FSTMIITLSYY1162 pKa = 10.66LIFRR1166 pKa = 11.84RR1167 pKa = 11.84RR1168 pKa = 11.84KK1169 pKa = 9.11NLL1171 pKa = 3.25

Molecular weight: 126.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5XP78|A0A2H5XP78_9BACT Alanine racemase OS=bacterium HR19 OX=2035414 GN=dadX PE=3 SV=1
MM1 pKa = 7.87DD2 pKa = 3.64VFIRR6 pKa = 11.84RR7 pKa = 11.84ILANTLDD14 pKa = 3.91RR15 pKa = 11.84SVIIYY20 pKa = 10.16ISALISLVGKK30 pKa = 10.47INFLGFILMFLFFDD44 pKa = 4.51FTTSVMFRR52 pKa = 11.84WAFEE56 pKa = 4.04RR57 pKa = 11.84TPGDD61 pKa = 3.24IIFGIRR67 pKa = 11.84ISKK70 pKa = 9.52KK71 pKa = 8.27KK72 pKa = 10.08HH73 pKa = 4.49GAIHH77 pKa = 6.91LKK79 pKa = 10.48LALRR83 pKa = 11.84WIMGFISPLTGFLLHH98 pKa = 6.93IPAGNWRR105 pKa = 11.84SLCDD109 pKa = 3.59TLAGIQFEE117 pKa = 4.52RR118 pKa = 11.84VV119 pKa = 2.99

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1704

0

1704

576888

31

2244

338.5

38.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.267 ± 0.047

0.963 ± 0.02

4.895 ± 0.037

8.372 ± 0.068

6.482 ± 0.07

6.559 ± 0.047

1.281 ± 0.019

9.587 ± 0.055

9.4 ± 0.069

8.873 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.852 ± 0.024

3.841 ± 0.038

3.954 ± 0.034

2.294 ± 0.026

4.58 ± 0.038

7.164 ± 0.063

3.848 ± 0.038

6.464 ± 0.045

0.954 ± 0.019

3.37 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski