Mycoplasma synoviae (strain 53)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis; Mycoplasmopsis synoviae

Average proteome isoelectric point is 7.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 679 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4A6D1|Q4A6D1_MYCS5 Excinuclease ABC subunit C OS=Mycoplasma synoviae (strain 53) OX=262723 GN=uvrC PE=4 SV=1
MM1 pKa = 7.85SDD3 pKa = 2.69TADD6 pKa = 3.27IVNEE10 pKa = 4.1LDD12 pKa = 3.58LPGDD16 pKa = 3.73FEE18 pKa = 4.49IVKK21 pKa = 10.51LGALALEE28 pKa = 4.49STNLVLTVLSKK39 pKa = 10.64IQIDD43 pKa = 3.59GTYY46 pKa = 9.56IYY48 pKa = 9.83TYY50 pKa = 10.76VSAINYY56 pKa = 7.36EE57 pKa = 4.35LNKK60 pKa = 9.96QDD62 pKa = 5.83DD63 pKa = 4.27MVLLSRR69 pKa = 11.84LFSQLKK75 pKa = 9.55QDD77 pKa = 4.71ADD79 pKa = 3.74NLSWWRR85 pKa = 11.84KK86 pKa = 7.75YY87 pKa = 11.23LLGGWIISEE96 pKa = 4.35KK97 pKa = 10.91ANEE100 pKa = 4.13LAQKK104 pKa = 6.97TTDD107 pKa = 3.53YY108 pKa = 10.59YY109 pKa = 11.26QEE111 pKa = 5.35AINKK115 pKa = 8.36YY116 pKa = 9.11NNSIQAQQQYY126 pKa = 11.06SDD128 pKa = 4.44YY129 pKa = 10.66EE130 pKa = 3.99NQIYY134 pKa = 10.09QSYY137 pKa = 10.86NSYY140 pKa = 11.31

Molecular weight: 16.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4A5J8|Q4A5J8_MYCS5 tRNA pseudouridine(55) synthase OS=Mycoplasma synoviae (strain 53) OX=262723 GN=truB PE=3 SV=1
MM1 pKa = 7.75ANPTQIYY8 pKa = 10.43SRR10 pKa = 11.84DD11 pKa = 3.8SKK13 pKa = 9.86WRR15 pKa = 11.84RR16 pKa = 11.84GVMRR20 pKa = 11.84SLVSEE25 pKa = 4.31LLLHH29 pKa = 6.92GRR31 pKa = 11.84IQTTLTRR38 pKa = 11.84AKK40 pKa = 9.56EE41 pKa = 4.23VRR43 pKa = 11.84GHH45 pKa = 5.96AEE47 pKa = 3.76KK48 pKa = 10.97LITKK52 pKa = 10.18AKK54 pKa = 9.0AQSLASRR61 pKa = 11.84RR62 pKa = 11.84HH63 pKa = 4.95AASFLRR69 pKa = 11.84PLVTKK74 pKa = 10.38DD75 pKa = 3.17NKK77 pKa = 10.11TLLQEE82 pKa = 4.46LFDD85 pKa = 4.73TIAPKK90 pKa = 10.91YY91 pKa = 9.2KK92 pKa = 10.14DD93 pKa = 3.36RR94 pKa = 11.84PGGYY98 pKa = 7.49TRR100 pKa = 11.84IYY102 pKa = 10.29KK103 pKa = 10.08LPKK106 pKa = 9.39RR107 pKa = 11.84DD108 pKa = 3.53GDD110 pKa = 4.0STRR113 pKa = 11.84MALIEE118 pKa = 4.07LVV120 pKa = 3.37

Molecular weight: 13.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

679

0

679

236649

37

2636

348.5

39.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.851 ± 0.12

0.4 ± 0.019

5.251 ± 0.072

6.316 ± 0.102

5.899 ± 0.111

4.459 ± 0.099

1.237 ± 0.037

7.906 ± 0.166

10.39 ± 0.122

10.192 ± 0.111

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.604 ± 0.037

7.889 ± 0.13

2.707 ± 0.059

3.421 ± 0.053

2.705 ± 0.047

7.316 ± 0.067

5.307 ± 0.104

6.068 ± 0.096

0.922 ± 0.026

4.16 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski