Burkholderia phage BcepNazgul

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nazgulvirus; Burkholderia virus BcepNazgul

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6UYK9|Q6UYK9_9CAUD Uncharacterized protein OS=Burkholderia phage BcepNazgul OX=242861 GN=Nazgul31 PE=4 SV=2
MM1 pKa = 7.58SNEE4 pKa = 4.08PKK6 pKa = 10.35DD7 pKa = 3.41AAGIITLSIQVSRR20 pKa = 11.84QLCEE24 pKa = 3.89DD25 pKa = 3.51TMDD28 pKa = 4.0AAAHH32 pKa = 5.37GVEE35 pKa = 4.59YY36 pKa = 9.45WAHH39 pKa = 6.01AFEE42 pKa = 5.51HH43 pKa = 6.8PGCGPEE49 pKa = 4.04TLNYY53 pKa = 10.19AIEE56 pKa = 4.18EE57 pKa = 4.46DD58 pKa = 4.21EE59 pKa = 4.67PSSGEE64 pKa = 3.96TPKK67 pKa = 11.0NFVISPEE74 pKa = 4.33AIVKK78 pKa = 8.88GIQAIMHH85 pKa = 6.4PAFSVRR91 pKa = 11.84ADD93 pKa = 2.99IRR95 pKa = 11.84GTLFSALVEE104 pKa = 4.6DD105 pKa = 4.81DD106 pKa = 4.23ASNIDD111 pKa = 4.08IEE113 pKa = 4.18IADD116 pKa = 4.84IIVQAAMFGEE126 pKa = 4.84IIYY129 pKa = 10.5GG130 pKa = 3.55

Molecular weight: 14.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6UYL5|Q6UYL5_9CAUD Uncharacterized protein OS=Burkholderia phage BcepNazgul OX=242861 GN=Nazgul25 PE=4 SV=1
MM1 pKa = 7.49GGPYY5 pKa = 10.27FFRR8 pKa = 11.84DD9 pKa = 3.52GSQYY13 pKa = 10.48TGHH16 pKa = 5.71QVVNFSVQKK25 pKa = 9.62GQGKK29 pKa = 8.8VRR31 pKa = 11.84IRR33 pKa = 11.84ARR35 pKa = 11.84CKK37 pKa = 9.46EE38 pKa = 4.15CNGRR42 pKa = 11.84PGSHH46 pKa = 6.49HH47 pKa = 6.68WPHH50 pKa = 6.07GRR52 pKa = 11.84CGACKK57 pKa = 9.33GTGINASYY65 pKa = 10.46SWVSVYY71 pKa = 10.51SAEE74 pKa = 5.03RR75 pKa = 11.84LNEE78 pKa = 3.91LNASKK83 pKa = 10.3HH84 pKa = 5.53RR85 pKa = 11.84KK86 pKa = 8.94HH87 pKa = 6.02MALSALALLMVKK99 pKa = 9.98RR100 pKa = 11.84DD101 pKa = 3.43FRR103 pKa = 11.84QFLRR107 pKa = 11.84QRR109 pKa = 11.84DD110 pKa = 3.67RR111 pKa = 11.84LLTDD115 pKa = 3.34MQTFAPKK122 pKa = 10.22SEE124 pKa = 3.82NLQLLLAKK132 pKa = 10.13VNQGTILTPTEE143 pKa = 3.35IRR145 pKa = 11.84QALTLVNTVRR155 pKa = 11.84SQFYY159 pKa = 10.17VAASCARR166 pKa = 3.5

Molecular weight: 18.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

18216

48

1270

249.5

27.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.517 ± 0.649

1.147 ± 0.135

6.346 ± 0.222

5.693 ± 0.379

3.612 ± 0.132

7.439 ± 0.4

1.938 ± 0.169

4.617 ± 0.168

4.957 ± 0.363

7.828 ± 0.353

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.717 ± 0.162

3.843 ± 0.141

4.864 ± 0.273

3.931 ± 0.228

6.653 ± 0.342

5.523 ± 0.274

6.132 ± 0.463

6.527 ± 0.258

1.663 ± 0.158

3.052 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski