Metabacillus indicus (Bacillus indicus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Metabacillus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3781 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A084GX46|A0A084GX46_METID Sporulation protein OS=Metabacillus indicus OX=246786 GN=GS18_0212455 PE=4 SV=1
MM1 pKa = 7.48TSLSGNSGIFTGTNTQYY18 pKa = 11.63DD19 pKa = 3.79WTVSMKK25 pKa = 10.67SNYY28 pKa = 9.91GFGKK32 pKa = 10.41IMGANNLAVDD42 pKa = 4.5TVNIIHH48 pKa = 7.72DD49 pKa = 4.66DD50 pKa = 3.82DD51 pKa = 6.05FMDD54 pKa = 5.58SDD56 pKa = 4.33FTQNQGSNDD65 pKa = 3.44QPVYY69 pKa = 10.7QSS71 pKa = 3.04

Molecular weight: 7.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A084GNG4|A0A084GNG4_METID Sporulation initiation inhibitor Soj OS=Metabacillus indicus OX=246786 GN=GS18_0215760 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.21QPNNRR10 pKa = 11.84KK11 pKa = 9.13HH12 pKa = 6.54SKK14 pKa = 8.73VHH16 pKa = 5.66GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.48NGRR28 pKa = 11.84KK29 pKa = 9.07VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3781

0

3781

1086472

37

2007

287.4

32.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.878 ± 0.043

0.693 ± 0.012

5.146 ± 0.028

7.735 ± 0.051

4.579 ± 0.029

7.161 ± 0.035

2.119 ± 0.019

7.201 ± 0.041

6.944 ± 0.037

9.908 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.877 ± 0.019

3.839 ± 0.025

3.641 ± 0.023

3.509 ± 0.028

4.087 ± 0.032

6.252 ± 0.031

5.222 ± 0.023

6.812 ± 0.033

0.981 ± 0.014

3.417 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski