Methylophilales bacterium MBRS-H7

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; unclassified Nitrosomonadales; OM43 clade

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1317 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4JA03|A0A0H4JA03_9PROT Uncharacterized protein OS=Methylophilales bacterium MBRS-H7 OX=1623450 GN=VI33_00670 PE=4 SV=1
MM1 pKa = 7.37TYY3 pKa = 10.71VVTEE7 pKa = 3.67NCIQCKK13 pKa = 8.64YY14 pKa = 8.09TDD16 pKa = 4.36CVDD19 pKa = 3.61VCPVDD24 pKa = 4.53CFVEE28 pKa = 4.96GPNFLAIDD36 pKa = 3.86PEE38 pKa = 4.21EE39 pKa = 5.95CIDD42 pKa = 3.78CTLCVAEE49 pKa = 5.38CPAEE53 pKa = 4.5AIFAEE58 pKa = 4.6DD59 pKa = 5.49DD60 pKa = 3.81VPEE63 pKa = 4.55DD64 pKa = 3.23QLDD67 pKa = 4.22FIEE70 pKa = 5.16INAKK74 pKa = 9.12LAKK77 pKa = 8.49VWPIISEE84 pKa = 4.3RR85 pKa = 11.84KK86 pKa = 10.12DD87 pKa = 3.59PLPDD91 pKa = 4.26ADD93 pKa = 4.16SFNGKK98 pKa = 8.39EE99 pKa = 4.46GKK101 pKa = 9.77RR102 pKa = 11.84EE103 pKa = 3.94LLIEE107 pKa = 4.14EE108 pKa = 4.43

Molecular weight: 12.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4IXL4|A0A0H4IXL4_9PROT Uncharacterized protein OS=Methylophilales bacterium MBRS-H7 OX=1623450 GN=VI33_03025 PE=4 SV=1
MM1 pKa = 6.74ATYY4 pKa = 10.4RR5 pKa = 11.84KK6 pKa = 9.82RR7 pKa = 11.84NNKK10 pKa = 6.97WQVRR14 pKa = 11.84IQRR17 pKa = 11.84RR18 pKa = 11.84NNPPISKK25 pKa = 8.44TFVYY29 pKa = 10.61KK30 pKa = 10.61EE31 pKa = 4.01DD32 pKa = 3.47AKK34 pKa = 11.11VWARR38 pKa = 11.84QQEE41 pKa = 4.1RR42 pKa = 11.84EE43 pKa = 3.74IDD45 pKa = 3.57LGIEE49 pKa = 4.14VANQDD54 pKa = 3.32IALKK58 pKa = 9.53TLLQRR63 pKa = 11.84YY64 pKa = 7.27QKK66 pKa = 10.66EE67 pKa = 3.66ILPTKK72 pKa = 10.16KK73 pKa = 9.79HH74 pKa = 5.68PQPDD78 pKa = 3.3NYY80 pKa = 10.38RR81 pKa = 11.84INKK84 pKa = 8.84ILLHH88 pKa = 6.52PIVSLKK94 pKa = 9.75MRR96 pKa = 11.84TIKK99 pKa = 10.31SQHH102 pKa = 5.74ISSFRR107 pKa = 11.84DD108 pKa = 3.28QLIKK112 pKa = 10.35EE113 pKa = 4.36LKK115 pKa = 9.63SPNTIRR121 pKa = 11.84LYY123 pKa = 10.9LAILSHH129 pKa = 7.06LFTIARR135 pKa = 11.84TEE137 pKa = 3.8WGFDD141 pKa = 3.32NLNNPVLKK149 pKa = 10.04IRR151 pKa = 11.84RR152 pKa = 11.84PRR154 pKa = 11.84LPQSRR159 pKa = 11.84DD160 pKa = 2.99TRR162 pKa = 11.84LSDD165 pKa = 4.34DD166 pKa = 4.87DD167 pKa = 3.34IHH169 pKa = 7.42RR170 pKa = 11.84ICQHH174 pKa = 5.44SQSSLLPLLIHH185 pKa = 6.85IALDD189 pKa = 3.17TAMRR193 pKa = 11.84VSEE196 pKa = 4.14IVKK199 pKa = 10.28LRR201 pKa = 11.84VNDD204 pKa = 3.7CDD206 pKa = 3.79FKK208 pKa = 11.52KK209 pKa = 10.85RR210 pKa = 11.84MITVRR215 pKa = 11.84NTKK218 pKa = 9.91NYY220 pKa = 7.94HH221 pKa = 6.09DD222 pKa = 5.07RR223 pKa = 11.84YY224 pKa = 10.59IPMTNKK230 pKa = 9.03VAKK233 pKa = 9.84ILQHH237 pKa = 3.95QTRR240 pKa = 11.84LQGEE244 pKa = 4.23RR245 pKa = 11.84LFNITSHH252 pKa = 5.8AVSVAFYY259 pKa = 9.58RR260 pKa = 11.84ACKK263 pKa = 9.76RR264 pKa = 11.84AGIPNASFHH273 pKa = 5.65TLRR276 pKa = 11.84HH277 pKa = 5.26EE278 pKa = 4.07AVSRR282 pKa = 11.84FFEE285 pKa = 4.53KK286 pKa = 10.64GLNPMEE292 pKa = 4.18VATISGHH299 pKa = 6.37QSMQVLKK306 pKa = 10.18RR307 pKa = 11.84YY308 pKa = 6.64THH310 pKa = 6.2IQTTHH315 pKa = 6.88LLGKK319 pKa = 8.87MMRR322 pKa = 3.96

Molecular weight: 37.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1317

0

1317

403159

50

1541

306.1

34.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.292 ± 0.069

0.92 ± 0.019

5.891 ± 0.052

6.286 ± 0.075

5.049 ± 0.066

6.113 ± 0.07

2.084 ± 0.032

8.967 ± 0.066

8.047 ± 0.077

9.698 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.03

5.978 ± 0.06

3.502 ± 0.038

3.626 ± 0.036

3.585 ± 0.043

6.808 ± 0.052

4.709 ± 0.037

5.926 ± 0.056

0.911 ± 0.021

3.215 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski