Methylobacillus sp. MM3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Methylophilaceae; Methylobacillus; unclassified Methylobacillus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2816 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A9NJW5|A0A1A9NJW5_9PROT Cytochrome C OS=Methylobacillus sp. MM3 OX=1848039 GN=A7976_01535 PE=4 SV=1
MM1 pKa = 7.47SNDD4 pKa = 4.07PIAKK8 pKa = 9.69VLFRR12 pKa = 11.84VPDD15 pKa = 3.93DD16 pKa = 4.05EE17 pKa = 6.41GSATVEE23 pKa = 4.13MLWTIPLGDD32 pKa = 3.98EE33 pKa = 4.69RR34 pKa = 11.84YY35 pKa = 10.43KK36 pKa = 10.99LDD38 pKa = 3.47NSPFYY43 pKa = 10.8AYY45 pKa = 10.01GVSWQDD51 pKa = 3.08TVFAPVDD58 pKa = 3.66AQEE61 pKa = 4.58GLPTFQSVVAKK72 pKa = 10.26SGNRR76 pKa = 11.84TVRR79 pKa = 11.84IILDD83 pKa = 3.7PPVSPGNTSDD93 pKa = 3.81QVLQGLVALGCSYY106 pKa = 11.15EE107 pKa = 4.24GANPGYY113 pKa = 9.35MSVNIPPGIEE123 pKa = 3.86LQQVCSYY130 pKa = 10.81LVQQDD135 pKa = 4.27AQWEE139 pKa = 4.52HH140 pKa = 7.09ADD142 pKa = 3.51PTYY145 pKa = 11.17EE146 pKa = 4.7SLFPDD151 pKa = 3.85EE152 pKa = 4.7AA153 pKa = 5.55

Molecular weight: 16.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A9NKL5|A0A1A9NKL5_9PROT HTH tetR-type domain-containing protein OS=Methylobacillus sp. MM3 OX=1848039 GN=A7976_13040 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 8.87VRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.65GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84ARR41 pKa = 11.84LAVV44 pKa = 3.42

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2816

0

2816

889654

23

1933

315.9

34.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.69 ± 0.061

0.922 ± 0.016

5.339 ± 0.038

6.216 ± 0.042

3.76 ± 0.032

7.662 ± 0.037

2.453 ± 0.024

5.602 ± 0.033

4.228 ± 0.039

10.725 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.023

3.285 ± 0.027

4.699 ± 0.028

3.914 ± 0.03

6.165 ± 0.041

5.498 ± 0.029

5.008 ± 0.032

7.078 ± 0.035

1.312 ± 0.022

2.699 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski