Human associated gemykibivirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus humas1

Average proteome isoelectric point is 8.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2C4F9|A0A0S2C4F9_9VIRU Putative ORF3 protein OS=Human associated gemykibivirus 1 OX=2004487 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.7CNNEE5 pKa = 4.03LCLLFDD11 pKa = 5.1FKK13 pKa = 11.05HH14 pKa = 5.68GTPCSPIHH22 pKa = 5.93NVEE25 pKa = 4.0TSILLQWSIILRR37 pKa = 11.84DD38 pKa = 3.74LEE40 pKa = 4.66LNALSAEE47 pKa = 5.07RR48 pKa = 11.84ITQMGGLHH56 pKa = 6.26LHH58 pKa = 6.56AFVDD62 pKa = 4.51FGVKK66 pKa = 9.88YY67 pKa = 8.61RR68 pKa = 11.84TRR70 pKa = 11.84NARR73 pKa = 11.84AFDD76 pKa = 3.62VEE78 pKa = 4.65GFHH81 pKa = 7.21PNVSPSRR88 pKa = 11.84GTPEE92 pKa = 5.32DD93 pKa = 3.67GFDD96 pKa = 3.53YY97 pKa = 10.7AIKK100 pKa = 10.9DD101 pKa = 3.51GDD103 pKa = 4.01VVAGGLEE110 pKa = 4.0RR111 pKa = 11.84PAGSRR116 pKa = 11.84VDD118 pKa = 3.35ATGGVWPEE126 pKa = 3.91IINAKK131 pKa = 10.22DD132 pKa = 3.44EE133 pKa = 4.57SEE135 pKa = 4.12FWSLCEE141 pKa = 3.9SLAPRR146 pKa = 11.84SLVTSFTQLRR156 pKa = 11.84AYY158 pKa = 9.71AAWKK162 pKa = 9.67FPAIRR167 pKa = 11.84EE168 pKa = 4.18PYY170 pKa = 7.05EE171 pKa = 3.87TPEE174 pKa = 4.16GVNIDD179 pKa = 3.83TSWVVEE185 pKa = 3.91LDD187 pKa = 2.93NWVQQNLGRR196 pKa = 11.84APRR199 pKa = 11.84SSAHH203 pKa = 5.92YY204 pKa = 10.1KK205 pKa = 9.61SEE207 pKa = 3.6CRR209 pKa = 11.84LRR211 pKa = 11.84YY212 pKa = 7.15TEE214 pKa = 3.92RR215 pKa = 11.84RR216 pKa = 11.84KK217 pKa = 10.65SLVVYY222 pKa = 10.08GPSRR226 pKa = 11.84MGKK229 pKa = 7.33TIWARR234 pKa = 11.84SLGNHH239 pKa = 7.12AYY241 pKa = 10.41FGGLFSLDD249 pKa = 3.69EE250 pKa = 5.02NIDD253 pKa = 3.52GAEE256 pKa = 3.86YY257 pKa = 10.43AIFDD261 pKa = 4.21DD262 pKa = 4.25FGGIKK267 pKa = 10.32FLPSYY272 pKa = 10.27KK273 pKa = 10.0FWLGHH278 pKa = 4.73QKK280 pKa = 9.95QFYY283 pKa = 8.85VTDD286 pKa = 3.8KK287 pKa = 10.93YY288 pKa = 10.22RR289 pKa = 11.84GKK291 pKa = 10.64RR292 pKa = 11.84LVHH295 pKa = 6.25WARR298 pKa = 11.84PSIWLSNSDD307 pKa = 3.6PRR309 pKa = 11.84DD310 pKa = 3.43EE311 pKa = 5.63LGVDD315 pKa = 4.0TEE317 pKa = 4.54WLNANCDD324 pKa = 3.81FVYY327 pKa = 10.57LDD329 pKa = 4.01SPIVSS334 pKa = 3.68

Molecular weight: 37.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2C4K3|A0A0S2C4K3_9VIRU Capsid protein OS=Human associated gemykibivirus 1 OX=2004487 GN=Cap PE=4 SV=1
MM1 pKa = 7.1SVARR5 pKa = 11.84RR6 pKa = 11.84SVGRR10 pKa = 11.84TRR12 pKa = 11.84KK13 pKa = 9.36AFRR16 pKa = 11.84RR17 pKa = 11.84GGRR20 pKa = 11.84ATRR23 pKa = 11.84SRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84STRR30 pKa = 11.84FSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84VRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84PMTARR46 pKa = 11.84RR47 pKa = 11.84ILNVTSRR54 pKa = 11.84KK55 pKa = 9.84KK56 pKa = 10.06VDD58 pKa = 3.09NMLPIVVDD66 pKa = 3.77EE67 pKa = 4.46EE68 pKa = 4.95SVVTVGPFSSPSPLLCLFVPNARR91 pKa = 11.84DD92 pKa = 3.21TRR94 pKa = 11.84TPITNPAVRR103 pKa = 11.84NSSDD107 pKa = 2.87IFAVGYY113 pKa = 9.27KK114 pKa = 10.08EE115 pKa = 4.36KK116 pKa = 10.86VRR118 pKa = 11.84LDD120 pKa = 3.42VMGGGTFMWRR130 pKa = 11.84RR131 pKa = 11.84IVFMLKK137 pKa = 10.38GDD139 pKa = 3.86DD140 pKa = 3.74LRR142 pKa = 11.84RR143 pKa = 11.84FMDD146 pKa = 3.47SSNSGNVPAQLFDD159 pKa = 3.28QTTEE163 pKa = 4.13GGCRR167 pKa = 11.84RR168 pKa = 11.84VIGPLLGVTNAQEE181 pKa = 4.12EE182 pKa = 4.31LQKK185 pKa = 11.12YY186 pKa = 6.86VFRR189 pKa = 11.84GQEE192 pKa = 3.87DD193 pKa = 4.01VDD195 pKa = 3.48WADD198 pKa = 3.59QFTAPIDD205 pKa = 3.74TRR207 pKa = 11.84RR208 pKa = 11.84VTVKK212 pKa = 9.27YY213 pKa = 10.99DD214 pKa = 2.89KK215 pKa = 10.55MRR217 pKa = 11.84VIRR220 pKa = 11.84PGNDD224 pKa = 2.5TGASRR229 pKa = 11.84LYY231 pKa = 10.58RR232 pKa = 11.84FWHH235 pKa = 6.51PIRR238 pKa = 11.84RR239 pKa = 11.84TISYY243 pKa = 10.58EE244 pKa = 3.91DD245 pKa = 4.05DD246 pKa = 3.97LEE248 pKa = 6.14SDD250 pKa = 4.19VVGDD254 pKa = 4.43RR255 pKa = 11.84PFSTAGLRR263 pKa = 11.84GVGDD267 pKa = 4.13MYY269 pKa = 11.69VMDD272 pKa = 4.89IMAITNLTPDD282 pKa = 4.11APQTSYY288 pKa = 11.1RR289 pKa = 11.84FSPEE293 pKa = 3.23GSFYY297 pKa = 9.4WHH299 pKa = 6.64EE300 pKa = 4.04RR301 pKa = 3.36

Molecular weight: 34.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

829

194

334

276.3

31.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.669 ± 0.567

1.568 ± 0.359

5.669 ± 1.095

5.428 ± 0.683

4.343 ± 0.767

7.358 ± 0.222

2.533 ± 0.803

4.101 ± 0.353

3.378 ± 0.241

8.323 ± 1.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.895 ± 0.957

3.86 ± 0.299

4.825 ± 0.466

2.774 ± 0.509

10.736 ± 2.022

8.926 ± 1.188

5.428 ± 0.757

6.634 ± 1.119

2.413 ± 0.472

3.136 ± 0.351
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski