Elderberry carlavirus D

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus; Sambucus virus D

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7M9C7|A0A0A7M9C7_9VIRU Replicase polyprotein OS=Elderberry carlavirus D OX=1569055 PE=3 SV=1
MM1 pKa = 8.07DD2 pKa = 5.25CLINLALGCGFEE14 pKa = 4.12RR15 pKa = 11.84VSVNHH20 pKa = 6.37NGVLVFNCVPGAGKK34 pKa = 8.48STLIRR39 pKa = 11.84KK40 pKa = 8.61ALHH43 pKa = 5.81KK44 pKa = 10.49DD45 pKa = 2.97SRR47 pKa = 11.84FVAFTFGKK55 pKa = 10.18ADD57 pKa = 3.46PVGQRR62 pKa = 11.84GRR64 pKa = 11.84SILPAALFDD73 pKa = 4.85SEE75 pKa = 4.53ATCGKK80 pKa = 9.99LVLVDD85 pKa = 5.01EE86 pKa = 4.57YY87 pKa = 11.57TEE89 pKa = 4.5GDD91 pKa = 3.26WRR93 pKa = 11.84ALGACAIFGDD103 pKa = 4.14PQQASVNRR111 pKa = 11.84LWPEE115 pKa = 3.73PNFVCLLSKK124 pKa = 10.82RR125 pKa = 11.84FGAQTAALLRR135 pKa = 11.84NLGHH139 pKa = 6.13EE140 pKa = 4.5VYY142 pKa = 10.35SEE144 pKa = 4.27KK145 pKa = 10.84EE146 pKa = 4.35DD147 pKa = 3.37IVEE150 pKa = 3.91WSGVFQGEE158 pKa = 3.93PRR160 pKa = 11.84GLVLAYY166 pKa = 9.18EE167 pKa = 4.35PEE169 pKa = 4.4VIEE172 pKa = 5.6LIKK175 pKa = 11.19DD176 pKa = 3.13HH177 pKa = 7.45DD178 pKa = 5.13LDD180 pKa = 4.3FLHH183 pKa = 7.03PNCCRR188 pKa = 11.84GITVEE193 pKa = 4.35HH194 pKa = 5.6VTFITSLDD202 pKa = 3.64HH203 pKa = 7.53FDD205 pKa = 3.6VNRR208 pKa = 11.84PDD210 pKa = 3.85LQYY213 pKa = 11.61LCLTRR218 pKa = 11.84HH219 pKa = 5.79SKK221 pKa = 10.61SLLILSPNATLGTAA235 pKa = 3.77

Molecular weight: 25.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7MB71|A0A0A7MB71_9VIRU Capsid protein OS=Elderberry carlavirus D OX=1569055 PE=3 SV=1
MM1 pKa = 7.68AGEE4 pKa = 4.44HH5 pKa = 5.01VQINPVVRR13 pKa = 11.84ALAEE17 pKa = 3.99AFEE20 pKa = 4.64VHH22 pKa = 7.05CSTNNNYY29 pKa = 10.53NYY31 pKa = 10.81YY32 pKa = 9.39SLCKK36 pKa = 10.24RR37 pKa = 11.84IVGLNKK43 pKa = 9.94EE44 pKa = 4.32VGTGRR49 pKa = 11.84SNMAKK54 pKa = 9.89RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84AAALGRR63 pKa = 11.84SPRR66 pKa = 11.84CGRR69 pKa = 11.84VAPGFYY75 pKa = 9.52FTTKK79 pKa = 10.47CNGRR83 pKa = 11.84TCFDD87 pKa = 3.19AFGRR91 pKa = 11.84RR92 pKa = 11.84SEE94 pKa = 4.01LLRR97 pKa = 11.84YY98 pKa = 9.46IKK100 pKa = 10.41FGQRR104 pKa = 11.84KK105 pKa = 7.2EE106 pKa = 3.89TDD108 pKa = 3.39APNDD112 pKa = 3.64DD113 pKa = 4.53ANRR116 pKa = 11.84PMM118 pKa = 5.17

Molecular weight: 13.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2811

66

1980

468.5

52.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.577 ± 0.823

3.095 ± 0.436

4.589 ± 0.375

7.044 ± 0.933

5.301 ± 0.626

6.688 ± 0.534

2.206 ± 0.498

4.554 ± 0.325

5.514 ± 0.785

10.103 ± 1.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.028 ± 0.378

4.447 ± 0.428

4.625 ± 0.799

2.775 ± 0.243

6.439 ± 0.656

7.364 ± 0.629

4.731 ± 0.698

6.581 ± 0.551

1.138 ± 0.151

3.202 ± 0.478
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski