Desulfocucumis palustris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfocucumis

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3959 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L2XM15|A0A2L2XM15_9FIRM Uncharacterized protein OS=Desulfocucumis palustris OX=1898651 GN=DCCM_4112 PE=4 SV=1
MM1 pKa = 7.54AKK3 pKa = 10.07KK4 pKa = 9.73PRR6 pKa = 11.84NPWCIRR12 pKa = 11.84YY13 pKa = 9.95ANIGYY18 pKa = 7.0YY19 pKa = 9.77HH20 pKa = 7.64RR21 pKa = 11.84NTVCLSANPAAVTVEE36 pKa = 3.88ANQTEE41 pKa = 4.59NVALTAEE48 pKa = 4.38KK49 pKa = 10.56DD50 pKa = 3.76GEE52 pKa = 4.48VVEE55 pKa = 4.9GVDD58 pKa = 4.26YY59 pKa = 11.22DD60 pKa = 3.92VTSADD65 pKa = 3.62EE66 pKa = 4.4TVATATEE73 pKa = 4.11ADD75 pKa = 3.74GVITVTGVRR84 pKa = 11.84EE85 pKa = 4.24GSTEE89 pKa = 4.1LTVTAAKK96 pKa = 10.25EE97 pKa = 4.29GEE99 pKa = 4.42EE100 pKa = 4.21FAAIAPIAVTVTEE113 pKa = 4.24GTIATVEE120 pKa = 3.91ITGAASVPVGTSVDD134 pKa = 3.63LTAVAKK140 pKa = 10.49DD141 pKa = 3.09AGGNVIADD149 pKa = 4.13AGAVTWAITSDD160 pKa = 3.51NADD163 pKa = 3.48NAVLTQSGKK172 pKa = 10.71FIATVAGSYY181 pKa = 9.06TVTATIGAVVGTATIQVSGIAAGVTFTASKK211 pKa = 9.49TSVVANATDD220 pKa = 3.41TADD223 pKa = 3.17ITVSVVDD230 pKa = 3.63TNGNVVTNFNGTAAPQRR247 pKa = 11.84TPVAGAQAVAVPATVTITNGVGTFTATSFAAGSIGTDD284 pKa = 3.08SLSITVGTLVSTNGQPIATSLNYY307 pKa = 10.89GNVQVSSVARR317 pKa = 11.84TATALTLSGPAYY329 pKa = 10.53VSANGNTQATLNVGYY344 pKa = 10.75ADD346 pKa = 3.76QNGAAMNAPAAQYY359 pKa = 8.23VTYY362 pKa = 9.63TLTGPASFQQGGAPVTTQSVFVPAAGGTVNIFAIMGQTGAITLTASAAGLTTATAQIQSVINTAATDD429 pKa = 3.36ITVTSEE435 pKa = 4.0TATLTAVCNADD446 pKa = 3.27NAGTNLPAGTAFNRR460 pKa = 11.84ITIQLVDD467 pKa = 3.77TNGLPVAGTDD477 pKa = 4.08ALNISDD483 pKa = 4.02NTTAASSLYY492 pKa = 9.61YY493 pKa = 8.63WTVNANGQPGVYY505 pKa = 10.01LGTTPQVSALVGGRR519 pKa = 11.84LQLAVLNTAVAATSPTITVRR539 pKa = 11.84DD540 pKa = 3.63TVLNVAKK547 pKa = 7.48TTTYY551 pKa = 11.0SYY553 pKa = 9.3VTSSASTATWVGQAIQTAMAGQSVTLTLQLQDD585 pKa = 3.0IAGNDD590 pKa = 3.45LQIANRR596 pKa = 11.84NVPVYY601 pKa = 10.08FLANAANATIAGSSAWTVLNPYY623 pKa = 10.31NVTTNAQGQATVTVAVPAASAGSAFTLTSALTGGVPAVVTVTAVNASAYY672 pKa = 8.59ATGLVLANTAVAAGSIPNPTTEE694 pKa = 4.5VITWPNGNMTAGQNLAAFVGSAINVLPVNAIGQVANATDD733 pKa = 4.36VIRR736 pKa = 11.84VTSSNEE742 pKa = 3.58NVLDD746 pKa = 3.62IAGVTNGFVEE756 pKa = 5.1GPFAVGPVPAITAVNSGTATLTFTNVTNPAVPAVTKK792 pKa = 10.14TITVVPATAPAAVLGMNPDD811 pKa = 3.16GTTNRR816 pKa = 11.84VYY818 pKa = 11.15NFTTAGVVGPFTLQAVDD835 pKa = 3.4GGNNLVAATVPITLTAVQVDD855 pKa = 4.78NILGTPGTPGIRR867 pKa = 11.84TSAAGSNVTSVTIPQGQASVQIWVDD892 pKa = 4.56GITLPSATAATAAVALNTLAPEE914 pKa = 4.21IVSAAINGDD923 pKa = 3.85TLVVTMNNPVTGTATAAAFDD943 pKa = 4.15VVIDD947 pKa = 4.17AAPAVNPTGDD957 pKa = 3.63GVISGNTITLTLAAPVTNANVVTLAYY983 pKa = 6.91TTDD986 pKa = 3.19SFLTTGYY993 pKa = 9.67QAPLATTIAPIAVTNNTPP1011 pKa = 3.02

Molecular weight: 100.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L2XFA6|A0A2L2XFA6_9FIRM N-acetyltransferase domain-containing protein OS=Desulfocucumis palustris OX=1898651 GN=DCCM_3932 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPKK8 pKa = 8.41NRR10 pKa = 11.84KK11 pKa = 7.86HH12 pKa = 6.2KK13 pKa = 10.07KK14 pKa = 7.69VHH16 pKa = 5.74GFLKK20 pKa = 10.48RR21 pKa = 11.84MSSPGGEE28 pKa = 3.76NVIKK32 pKa = 10.52RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.22GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3959

0

3959

1035266

37

6798

261.5

28.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.264 ± 0.053

1.218 ± 0.016

4.943 ± 0.031

6.741 ± 0.047

3.981 ± 0.026

8.203 ± 0.045

1.674 ± 0.015

7.059 ± 0.042

5.99 ± 0.038

10.03 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.76 ± 0.021

4.134 ± 0.032

4.304 ± 0.026

3.14 ± 0.027

5.487 ± 0.043

5.68 ± 0.038

4.818 ± 0.036

7.259 ± 0.036

0.996 ± 0.015

3.319 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski