Mycobacterium phage XianYue

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6T4M7|A0A4D6T4M7_9CAUD DNA primase OS=Mycobacterium phage XianYue OX=2572534 GN=57 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 9.31ITVWTLTVEE11 pKa = 4.26YY12 pKa = 10.24EE13 pKa = 4.59DD14 pKa = 3.95VLEE17 pKa = 4.71TYY19 pKa = 10.56AYY21 pKa = 5.66TTEE24 pKa = 4.12DD25 pKa = 2.99AAMQEE30 pKa = 3.98LRR32 pKa = 11.84QWFAADD38 pKa = 3.21HH39 pKa = 6.23DD40 pKa = 4.38QWKK43 pKa = 9.4SWHH46 pKa = 7.12DD47 pKa = 3.6EE48 pKa = 3.93PAPFTQEE55 pKa = 3.97EE56 pKa = 4.73LDD58 pKa = 4.16EE59 pKa = 4.54LTDD62 pKa = 4.13SQFQDD67 pKa = 3.68LLNDD71 pKa = 3.53YY72 pKa = 10.66DD73 pKa = 4.08VTNSIIPHH81 pKa = 5.39EE82 pKa = 4.12LEE84 pKa = 3.83II85 pKa = 6.17

Molecular weight: 10.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6T4H1|A0A4D6T4H1_9CAUD VIP2-like toxin OS=Mycobacterium phage XianYue OX=2572534 GN=2 PE=4 SV=1
MM1 pKa = 7.23SWAGSRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84HH10 pKa = 6.28DD11 pKa = 5.21LPPDD15 pKa = 3.07WEE17 pKa = 4.44LNYY20 pKa = 10.26RR21 pKa = 11.84LPVLRR26 pKa = 11.84DD27 pKa = 3.59ANWICEE33 pKa = 4.15LRR35 pKa = 11.84WNGCVGTATEE45 pKa = 3.96VDD47 pKa = 3.79HH48 pKa = 7.16IKK50 pKa = 10.59RR51 pKa = 11.84GNDD54 pKa = 3.11HH55 pKa = 6.61SRR57 pKa = 11.84SNLQAVCHH65 pKa = 6.16RR66 pKa = 11.84CHH68 pKa = 6.56AKK70 pKa = 10.26KK71 pKa = 10.66SSAEE75 pKa = 3.83GNARR79 pKa = 11.84KK80 pKa = 10.05AEE82 pKa = 3.99LKK84 pKa = 10.47ARR86 pKa = 11.84RR87 pKa = 11.84KK88 pKa = 10.3RR89 pKa = 11.84PDD91 pKa = 2.8QRR93 pKa = 11.84HH94 pKa = 5.52PGRR97 pKa = 11.84RR98 pKa = 3.43

Molecular weight: 11.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

16396

38

842

178.2

19.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.96 ± 0.383

0.909 ± 0.108

6.343 ± 0.181

6.953 ± 0.359

3.47 ± 0.171

8.215 ± 0.415

1.921 ± 0.163

5.087 ± 0.131

4.733 ± 0.258

8.258 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.129

3.367 ± 0.171

5.489 ± 0.23

3.537 ± 0.197

6.325 ± 0.343

5.166 ± 0.191

6.014 ± 0.189

7.038 ± 0.246

1.927 ± 0.126

2.854 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski