Candidatus Azoamicus ciliaticola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; Candidatus Azoamicus

Average proteome isoelectric point is 7.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 310 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J5JW86|A0A6J5JW86_9GAMM N(6)-L-threonylcarbamoyladenine synthase OS=Candidatus Azoamicus ciliaticola OX=2652803 GN=tsaD PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 9.7PCIIIKK8 pKa = 10.63GSDD11 pKa = 3.34DD12 pKa = 3.99NIEE15 pKa = 4.2DD16 pKa = 4.03FEE18 pKa = 4.67NKK20 pKa = 9.13IALALEE26 pKa = 3.89QGYY29 pKa = 9.1EE30 pKa = 3.97LSGEE34 pKa = 4.67LITHH38 pKa = 6.07VLNLDD43 pKa = 3.76GEE45 pKa = 4.57DD46 pKa = 4.36VIILLQPMFLSNDD59 pKa = 3.62SYY61 pKa = 11.9NEE63 pKa = 3.75NYY65 pKa = 10.65

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J5JVN3|A0A6J5JVN3_9GAMM Uncharacterized protein OS=Candidatus Azoamicus ciliaticola OX=2652803 GN=ESZ_00172 PE=4 SV=1
MM1 pKa = 7.57RR2 pKa = 11.84HH3 pKa = 6.02RR4 pKa = 11.84NIGRR8 pKa = 11.84YY9 pKa = 7.55FNRR12 pKa = 11.84NSEE15 pKa = 3.9QRR17 pKa = 11.84KK18 pKa = 9.14ALFLNLCKK26 pKa = 10.52DD27 pKa = 4.34LIKK30 pKa = 11.01YY31 pKa = 9.08GIIKK35 pKa = 7.03TTLEE39 pKa = 3.85KK40 pKa = 10.8AKK42 pKa = 9.92EE43 pKa = 3.75LRR45 pKa = 11.84GYY47 pKa = 9.91IEE49 pKa = 4.06PLITLSKK56 pKa = 10.61NDD58 pKa = 3.89SISNRR63 pKa = 11.84RR64 pKa = 11.84LVFKK68 pKa = 8.5TVRR71 pKa = 11.84DD72 pKa = 3.79KK73 pKa = 11.86NSVHH77 pKa = 6.94ILFSVLGKK85 pKa = 10.21RR86 pKa = 11.84YY87 pKa = 8.53VSRR90 pKa = 11.84PGGYY94 pKa = 7.86TRR96 pKa = 11.84ILKK99 pKa = 10.03YY100 pKa = 10.42GIRR103 pKa = 11.84RR104 pKa = 11.84GDD106 pKa = 3.75CATLAFIEE114 pKa = 4.47LVDD117 pKa = 4.42RR118 pKa = 11.84NINGG122 pKa = 3.28

Molecular weight: 14.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

310

0

310

91580

37

1354

295.4

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.696 ± 0.128

1.422 ± 0.054

4.972 ± 0.126

4.99 ± 0.145

6.927 ± 0.271

5.079 ± 0.144

1.435 ± 0.046

12.221 ± 0.247

10.525 ± 0.262

10.14 ± 0.131

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.963 ± 0.057

8.054 ± 0.193

2.445 ± 0.077

1.893 ± 0.064

2.851 ± 0.105

6.958 ± 0.127

4.283 ± 0.112

5.296 ± 0.163

0.717 ± 0.068

4.133 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski