Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Heliobacteriaceae; Heliomicrobium; Heliomicrobium modesticaldum

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2924 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0TBY8|B0TBY8_HELMI DNA repair protein radc OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) OX=498761 GN=radC PE=3 SV=1
MM1 pKa = 7.99DD2 pKa = 4.65MALDD6 pKa = 4.88DD7 pKa = 5.44PGDD10 pKa = 3.6DD11 pKa = 3.74HH12 pKa = 8.12ASLRR16 pKa = 11.84IDD18 pKa = 4.72DD19 pKa = 4.48PVCEE23 pKa = 4.25EE24 pKa = 4.22GGLAIACRR32 pKa = 11.84AGFTYY37 pKa = 10.9

Molecular weight: 3.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0THR4|B0THR4_HELMI Translation initiation factor IF-2 OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) OX=498761 GN=infB PE=3 SV=1
MM1 pKa = 7.28AHH3 pKa = 7.02AGAARR8 pKa = 11.84TRR10 pKa = 11.84AAEE13 pKa = 3.88AALRR17 pKa = 11.84TGTALTHH24 pKa = 6.71AGAAGAGTAEE34 pKa = 4.23AAFGTRR40 pKa = 11.84RR41 pKa = 11.84TGTAFAALAFRR52 pKa = 11.84TRR54 pKa = 11.84RR55 pKa = 11.84TRR57 pKa = 11.84AAFAALAFRR66 pKa = 11.84TRR68 pKa = 11.84RR69 pKa = 11.84TGAAFAALAFRR80 pKa = 11.84TRR82 pKa = 11.84RR83 pKa = 11.84TGTAFAALAFRR94 pKa = 11.84TRR96 pKa = 11.84RR97 pKa = 11.84TGAAFAALAFRR108 pKa = 11.84TRR110 pKa = 11.84RR111 pKa = 11.84TGAAFAALTFRR122 pKa = 11.84TRR124 pKa = 11.84RR125 pKa = 11.84TGAAFAALAFRR136 pKa = 11.84TRR138 pKa = 11.84RR139 pKa = 11.84TGAAFAALAFRR150 pKa = 11.84TRR152 pKa = 11.84RR153 pKa = 11.84TGAAFAALAFRR164 pKa = 11.84TRR166 pKa = 11.84RR167 pKa = 11.84TGAAFAALAFRR178 pKa = 11.84TRR180 pKa = 11.84RR181 pKa = 11.84TGAAFAALAFRR192 pKa = 11.84TRR194 pKa = 11.84RR195 pKa = 11.84TGAAFAALAFRR206 pKa = 11.84TRR208 pKa = 11.84RR209 pKa = 11.84TGAAFAALAFRR220 pKa = 11.84TGTAFTALTFRR231 pKa = 11.84TGRR234 pKa = 11.84TRR236 pKa = 11.84AVFAALAFRR245 pKa = 11.84AGRR248 pKa = 11.84SNVFIPLFFWPDD260 pKa = 3.05GAFMTTAFCRR270 pKa = 11.84CFQRR274 pKa = 11.84LFFRR278 pKa = 11.84GRR280 pKa = 11.84LMLLFTRR287 pKa = 11.84TGRR290 pKa = 11.84SRR292 pKa = 11.84ALAFKK297 pKa = 10.04QRR299 pKa = 11.84HH300 pKa = 4.99YY301 pKa = 11.25LLDD304 pKa = 3.16ITIFNGAQIILHH316 pKa = 6.38INTT319 pKa = 4.09

Molecular weight: 34.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2924

0

2924

852455

37

2976

291.5

32.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.958 ± 0.059

1.124 ± 0.019

5.183 ± 0.033

6.855 ± 0.049

3.708 ± 0.03

8.06 ± 0.051

1.91 ± 0.021

5.988 ± 0.041

4.862 ± 0.046

10.092 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.539 ± 0.021

3.009 ± 0.035

4.696 ± 0.034

3.656 ± 0.032

6.697 ± 0.057

5.243 ± 0.038

5.127 ± 0.061

7.423 ± 0.038

1.122 ± 0.017

2.748 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski