Rhizobium sp. RU36D

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5774 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W2EZS2|A0A1W2EZS2_9RHIZ 30S ribosomal protein S1 OS=Rhizobium sp. RU36D OX=1907415 GN=SAMN05880593_12740 PE=3 SV=1
MM1 pKa = 7.39MDD3 pKa = 3.85AASPMLDD10 pKa = 3.38VVSLLASNHH19 pKa = 5.7GEE21 pKa = 4.15QNEE24 pKa = 4.38TTASGGGLAGMLSAPVSDD42 pKa = 4.22YY43 pKa = 11.37PVMSDD48 pKa = 3.03AGYY51 pKa = 9.13PYY53 pKa = 10.13EE54 pKa = 6.22ADD56 pKa = 3.74MPSMDD61 pKa = 3.36QEE63 pKa = 3.49NWMRR67 pKa = 11.84VPANWLDD74 pKa = 3.44AMPPEE79 pKa = 5.73LYY81 pKa = 10.52EE82 pKa = 3.68QDD84 pKa = 3.49TRR86 pKa = 11.84EE87 pKa = 3.86EE88 pKa = 4.06DD89 pKa = 4.91AITACNSDD97 pKa = 4.01DD98 pKa = 3.61NGLYY102 pKa = 10.65RR103 pKa = 11.84MFRR106 pKa = 11.84NLSPP110 pKa = 3.81

Molecular weight: 12.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W2C8N0|A0A1W2C8N0_9RHIZ Uncharacterized protein OS=Rhizobium sp. RU36D OX=1907415 GN=SAMN05880593_107128 PE=4 SV=1
MM1 pKa = 7.56ANLVNEE7 pKa = 4.55LVTGVVEE14 pKa = 4.38SVIKK18 pKa = 10.52EE19 pKa = 3.94ILKK22 pKa = 9.01KK23 pKa = 7.71TTGKK27 pKa = 10.6RR28 pKa = 11.84STTKK32 pKa = 9.8RR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 8.77RR36 pKa = 11.84QQTRR40 pKa = 11.84SATTGRR46 pKa = 11.84FVRR49 pKa = 11.84KK50 pKa = 9.35KK51 pKa = 9.58ATSTTRR57 pKa = 11.84KK58 pKa = 7.39PAKK61 pKa = 8.93RR62 pKa = 11.84QQSRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84TARR71 pKa = 11.84ARR73 pKa = 11.84SRR75 pKa = 11.84QRR77 pKa = 11.84SRR79 pKa = 3.28

Molecular weight: 9.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5774

0

5774

1802097

27

6932

312.1

33.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.037 ± 0.047

0.806 ± 0.01

5.678 ± 0.025

5.723 ± 0.031

3.831 ± 0.023

8.348 ± 0.039

2.013 ± 0.015

5.637 ± 0.022

3.588 ± 0.028

10.109 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.682 ± 0.015

2.761 ± 0.021

4.897 ± 0.028

3.191 ± 0.022

6.676 ± 0.029

5.794 ± 0.032

5.332 ± 0.033

7.344 ± 0.026

1.269 ± 0.014

2.285 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski