Maize yellow dwarf virus RMV

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 8.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9U4P5|R9U4P5_9LUTE Movement protein OS=Maize yellow dwarf virus RMV OX=2170101 PE=3 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84CEE4 pKa = 3.58IHH6 pKa = 7.17FDD8 pKa = 3.35GTLTFAEE15 pKa = 4.63IPRR18 pKa = 11.84DD19 pKa = 3.34LTIRR23 pKa = 11.84LLEE26 pKa = 4.15FQTALRR32 pKa = 11.84TIACCVLNQRR42 pKa = 11.84YY43 pKa = 8.67DD44 pKa = 3.14AEE46 pKa = 4.41TLVRR50 pKa = 11.84SAFTILPLMLRR61 pKa = 11.84EE62 pKa = 5.64LPFTWEE68 pKa = 4.22FPSAVHH74 pKa = 6.69AAFAKK79 pKa = 10.19HH80 pKa = 6.1ALRR83 pKa = 11.84TAGRR87 pKa = 11.84IPPPMEE93 pKa = 5.38LIFHH97 pKa = 7.15GDD99 pKa = 3.69TPKK102 pKa = 10.91HH103 pKa = 5.5MYY105 pKa = 10.23RR106 pKa = 11.84DD107 pKa = 3.39FLYY110 pKa = 10.18RR111 pKa = 11.84VVTRR115 pKa = 11.84DD116 pKa = 2.89IAASLQRR123 pKa = 11.84SHH125 pKa = 6.65EE126 pKa = 4.02NFICGYY132 pKa = 10.44KK133 pKa = 10.13RR134 pKa = 11.84FQLRR138 pKa = 11.84LEE140 pKa = 4.45SVCRR144 pKa = 11.84RR145 pKa = 11.84VEE147 pKa = 3.9LLGDD151 pKa = 3.95TFAPKK156 pKa = 10.1HH157 pKa = 6.0IEE159 pKa = 3.9EE160 pKa = 5.02LSWSSRR166 pKa = 11.84TRR168 pKa = 11.84GGRR171 pKa = 11.84GAGARR176 pKa = 11.84RR177 pKa = 11.84RR178 pKa = 11.84LPARR182 pKa = 11.84CNLHH186 pKa = 7.22LEE188 pKa = 4.33LLDD191 pKa = 3.45IAALLGCYY199 pKa = 9.79NPGMQIYY206 pKa = 9.5VPDD209 pKa = 4.26VIDD212 pKa = 3.88RR213 pKa = 11.84LSLRR217 pKa = 11.84LYY219 pKa = 10.17NALGPAGFKK228 pKa = 10.7DD229 pKa = 4.42FWRR232 pKa = 11.84VAGYY236 pKa = 10.56DD237 pKa = 3.98HH238 pKa = 7.35LADD241 pKa = 3.52WDD243 pKa = 4.01SDD245 pKa = 3.56IEE247 pKa = 4.12ALSGSVIQKK256 pKa = 9.42EE257 pKa = 4.2LNLLL261 pKa = 3.89

Molecular weight: 29.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9U4P1|R9U4P1_9LUTE PO protein OS=Maize yellow dwarf virus RMV OX=2170101 PE=4 SV=1
MM1 pKa = 7.26NLGGRR6 pKa = 11.84RR7 pKa = 11.84NGRR10 pKa = 11.84RR11 pKa = 11.84GTRR14 pKa = 11.84LRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84VRR20 pKa = 11.84IARR23 pKa = 11.84TTQPMVVVAQTQRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84IRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84GRR45 pKa = 11.84PSGDD49 pKa = 2.84TSGGPRR55 pKa = 11.84GRR57 pKa = 11.84GGSRR61 pKa = 11.84EE62 pKa = 3.92TFVFSKK68 pKa = 11.01DD69 pKa = 3.54SIAGSASGKK78 pKa = 8.5LTFGASLSEE87 pKa = 4.16CAAFSGGILKK97 pKa = 10.27AYY99 pKa = 10.17HH100 pKa = 6.69EE101 pKa = 4.34YY102 pKa = 10.8KK103 pKa = 8.01ITKK106 pKa = 9.86VILEE110 pKa = 5.04FISEE114 pKa = 4.35APYY117 pKa = 9.24TAAGSIAYY125 pKa = 9.46EE126 pKa = 3.89LDD128 pKa = 3.22PHH130 pKa = 6.77NKK132 pKa = 9.92LSTLASTINKK142 pKa = 9.47FSIVKK147 pKa = 9.35GGKK150 pKa = 8.72RR151 pKa = 11.84AYY153 pKa = 9.0TSKK156 pKa = 10.86QIGGGVWRR164 pKa = 11.84DD165 pKa = 3.18SSEE168 pKa = 3.95DD169 pKa = 3.37QFAILYY175 pKa = 9.21KK176 pKa = 10.76GSGNSSVAGSFRR188 pKa = 11.84ITMEE192 pKa = 3.75VHH194 pKa = 4.91TQNPKK199 pKa = 10.13

Molecular weight: 21.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2964

193

1045

494.0

55.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.018 ± 0.437

1.754 ± 0.323

4.285 ± 0.401

5.364 ± 0.371

3.745 ± 0.299

7.085 ± 0.833

1.923 ± 0.269

4.791 ± 0.341

5.128 ± 0.548

9.615 ± 1.126

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.518 ± 0.126

3.88 ± 0.525

5.398 ± 0.633

3.981 ± 0.474

7.355 ± 1.007

9.717 ± 0.716

6.377 ± 0.519

6.174 ± 0.414

1.687 ± 0.214

3.171 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski