Nocardioides sp.

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4389 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S5AF19|A0A4S5AF19_9ACTN Biotin synthase OS=Nocardioides sp. OX=35761 GN=bioB PE=3 SV=1
MM1 pKa = 7.3NVTPFRR7 pKa = 11.84RR8 pKa = 11.84ALVPGGAALALILTACGGGAEE29 pKa = 4.51GGSGASGSVTVDD41 pKa = 3.35GSSTVYY47 pKa = 10.48PMSSAAAEE55 pKa = 4.05LLADD59 pKa = 4.09EE60 pKa = 5.03NSDD63 pKa = 3.41IEE65 pKa = 4.5VSVGFAGTGGGFEE78 pKa = 4.72KK79 pKa = 10.63FCSGDD84 pKa = 3.27TDD86 pKa = 2.98ISNASRR92 pKa = 11.84PIKK95 pKa = 10.53DD96 pKa = 4.01EE97 pKa = 3.9EE98 pKa = 4.14ATACTDD104 pKa = 3.06AGIEE108 pKa = 4.08YY109 pKa = 9.99TEE111 pKa = 4.81LLVATDD117 pKa = 3.44ALTVVVHH124 pKa = 6.89PDD126 pKa = 3.32LAVDD130 pKa = 4.3CLTTEE135 pKa = 4.03QVVEE139 pKa = 3.91LFGPNSKK146 pKa = 8.03VTKK149 pKa = 9.98WSDD152 pKa = 3.48LDD154 pKa = 3.73PSFPDD159 pKa = 3.19TKK161 pKa = 10.85VAGFIPGTDD170 pKa = 3.11SGTYY174 pKa = 10.12DD175 pKa = 3.39YY176 pKa = 10.64MAADD180 pKa = 3.71VVGDD184 pKa = 3.51EE185 pKa = 4.74SEE187 pKa = 4.16TLRR190 pKa = 11.84EE191 pKa = 4.13DD192 pKa = 3.93FEE194 pKa = 4.99SSEE197 pKa = 4.18DD198 pKa = 4.37DD199 pKa = 3.43NVLVQGVSGTEE210 pKa = 3.89GAVGFFGYY218 pKa = 9.22TYY220 pKa = 10.69FEE222 pKa = 4.47EE223 pKa = 4.63NADD226 pKa = 3.71KK227 pKa = 11.24VKK229 pKa = 10.75ALQIDD234 pKa = 4.03DD235 pKa = 4.24GQGCVAPSAEE245 pKa = 4.06TAQAGEE251 pKa = 4.43YY252 pKa = 9.36TPLARR257 pKa = 11.84PLFIYY262 pKa = 10.46VNNAKK267 pKa = 10.73YY268 pKa = 10.73NDD270 pKa = 3.64NEE272 pKa = 4.12AVKK275 pKa = 10.16AYY277 pKa = 9.39TDD279 pKa = 4.01FYY281 pKa = 10.94IEE283 pKa = 3.99NLAAIAKK290 pKa = 6.88GAKK293 pKa = 9.44YY294 pKa = 10.17IPLNDD299 pKa = 3.26EE300 pKa = 5.21DD301 pKa = 4.12YY302 pKa = 11.66AEE304 pKa = 4.4TKK306 pKa = 9.4TALEE310 pKa = 4.36GLAGG314 pKa = 3.71

Molecular weight: 32.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S4ZRU8|A0A4S4ZRU8_9ACTN SigE family RNA polymerase sigma factor OS=Nocardioides sp. OX=35761 GN=E7Z54_19300 PE=3 SV=1
RR1 pKa = 7.7RR2 pKa = 11.84PTGAGRR8 pKa = 11.84RR9 pKa = 11.84PRR11 pKa = 11.84PHH13 pKa = 7.24RR14 pKa = 11.84GGTAHH19 pKa = 7.27GAAAGRR25 pKa = 11.84APRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84GGARR34 pKa = 11.84EE35 pKa = 3.66PRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84PGRR42 pKa = 11.84RR43 pKa = 11.84ARR45 pKa = 11.84GRR47 pKa = 11.84ARR49 pKa = 11.84RR50 pKa = 11.84APGRR54 pKa = 11.84VAGARR59 pKa = 11.84GPQPP63 pKa = 3.25

Molecular weight: 6.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4389

0

4389

1381642

24

2468

314.8

33.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.922 ± 0.047

0.763 ± 0.01

6.414 ± 0.028

5.951 ± 0.032

2.818 ± 0.02

9.166 ± 0.031

2.222 ± 0.019

3.687 ± 0.026

1.976 ± 0.025

10.14 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.86 ± 0.013

1.846 ± 0.021

5.612 ± 0.024

2.785 ± 0.019

7.799 ± 0.041

5.237 ± 0.023

5.963 ± 0.039

9.297 ± 0.033

1.524 ± 0.016

2.016 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski