Mycobacterium phage HINdeR

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4JEU4|R4JEU4_9CAUD Holin OS=Mycobacterium phage HINdeR OX=1327770 GN=9 PE=4 SV=1
MM1 pKa = 7.48LLKK4 pKa = 10.73GFSPNGDD11 pKa = 3.27PTGALGNNGGLLGVIPGPPGPKK33 pKa = 9.74GDD35 pKa = 3.69GLEE38 pKa = 3.76IDD40 pKa = 4.59GVYY43 pKa = 8.42EE44 pKa = 4.2TYY46 pKa = 10.84SAFPSDD52 pKa = 5.29LGPEE56 pKa = 4.22DD57 pKa = 3.53EE58 pKa = 5.63GFAAFNQADD67 pKa = 3.97GQLYY71 pKa = 8.22MWSGTAWPAEE81 pKa = 3.94GTGAQFQGEE90 pKa = 4.47RR91 pKa = 11.84GPVGPQGPVGPQGPVGPQGPQGIQGYY117 pKa = 6.67TGPIGPEE124 pKa = 4.17GPKK127 pKa = 10.56GDD129 pKa = 4.89GIQIDD134 pKa = 4.37GQVATYY140 pKa = 11.18ADD142 pKa = 4.51LPTTGVAEE150 pKa = 4.46GDD152 pKa = 3.1TWLAATKK159 pKa = 10.11LYY161 pKa = 10.22RR162 pKa = 11.84YY163 pKa = 10.03SGTAWPPEE171 pKa = 4.22SQGAPVQGPQGLQGPQGPQGDD192 pKa = 4.27TGPTGPPGPTEE203 pKa = 3.54WLLITNRR210 pKa = 11.84PKK212 pKa = 10.34VAYY215 pKa = 10.28AEE217 pKa = 4.24VDD219 pKa = 3.62GVAADD224 pKa = 3.38VSLRR228 pKa = 11.84LMSEE232 pKa = 4.0AAYY235 pKa = 8.56QTVTVKK241 pKa = 10.75DD242 pKa = 3.75PKK244 pKa = 10.1TVYY247 pKa = 10.3LRR249 pKa = 11.84YY250 pKa = 10.02

Molecular weight: 25.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4JLE9|R4JLE9_9CAUD Uncharacterized protein OS=Mycobacterium phage HINdeR OX=1327770 GN=6 PE=4 SV=1
MM1 pKa = 7.14TWNTSDD7 pKa = 3.28RR8 pKa = 11.84SSRR11 pKa = 11.84LPADD15 pKa = 3.15WDD17 pKa = 3.52HH18 pKa = 7.07RR19 pKa = 11.84RR20 pKa = 11.84SQVLRR25 pKa = 11.84RR26 pKa = 11.84DD27 pKa = 4.15GYY29 pKa = 9.75QCQLRR34 pKa = 11.84LPGCLSEE41 pKa = 4.43ATEE44 pKa = 4.66ADD46 pKa = 3.99HH47 pKa = 7.02IQPGDD52 pKa = 3.29NHH54 pKa = 6.46SRR56 pKa = 11.84SNLQAACGRR65 pKa = 11.84CHH67 pKa = 6.64AKK69 pKa = 10.17KK70 pKa = 10.37SSRR73 pKa = 11.84EE74 pKa = 4.01GNDD77 pKa = 2.74KK78 pKa = 10.04KK79 pKa = 11.01RR80 pKa = 11.84RR81 pKa = 11.84MRR83 pKa = 11.84AARR86 pKa = 11.84FMPQEE91 pKa = 3.91RR92 pKa = 11.84HH93 pKa = 5.43PGSRR97 pKa = 3.35

Molecular weight: 11.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

16643

22

1123

198.1

21.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.548 ± 0.366

0.823 ± 0.114

6.345 ± 0.225

6.093 ± 0.293

3.359 ± 0.206

9.668 ± 0.746

2.061 ± 0.188

4.777 ± 0.143

4.32 ± 0.188

8.063 ± 0.382

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.136

3.245 ± 0.169

5.768 ± 0.345

3.924 ± 0.308

6.087 ± 0.374

5.294 ± 0.183

6.141 ± 0.252

7.246 ± 0.182

2.067 ± 0.16

2.746 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski