Pigmentiphaga sp. NML080357

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Pigmentiphaga; unclassified Pigmentiphaga

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5314 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A208Y1T2|A0A208Y1T2_9BURK Inosine-5'-monophosphate dehydrogenase OS=Pigmentiphaga sp. NML080357 OX=2008675 GN=guaB PE=3 SV=1
MM1 pKa = 7.85DD2 pKa = 6.75PILATLPPSLLALVEE17 pKa = 4.5GSLSNDD23 pKa = 2.79EE24 pKa = 4.32VSSDD28 pKa = 4.01EE29 pKa = 4.14EE30 pKa = 3.84MLAYY34 pKa = 10.24FIDD37 pKa = 3.95NGLTEE42 pKa = 4.41DD43 pKa = 3.76QARR46 pKa = 11.84QALTYY51 pKa = 10.19RR52 pKa = 11.84DD53 pKa = 3.41QYY55 pKa = 11.36LNNIYY60 pKa = 10.76LEE62 pKa = 4.48GFTPITSADD71 pKa = 3.8EE72 pKa = 4.11PLHH75 pKa = 6.08FNPHH79 pKa = 4.38TRR81 pKa = 11.84QFEE84 pKa = 3.94PDD86 pKa = 2.92

Molecular weight: 9.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A208XGN0|A0A208XGN0_9BURK ATP synthase epsilon chain OS=Pigmentiphaga sp. NML080357 OX=2008675 GN=atpC PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5314

0

5314

1716856

36

4487

323.1

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.121 ± 0.046

0.918 ± 0.011

5.34 ± 0.024

5.422 ± 0.031

3.368 ± 0.019

8.623 ± 0.038

2.239 ± 0.017

4.371 ± 0.021

2.854 ± 0.026

10.504 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.015

2.395 ± 0.017

5.635 ± 0.029

3.537 ± 0.021

7.748 ± 0.032

5.208 ± 0.021

4.888 ± 0.021

7.628 ± 0.026

1.397 ± 0.015

2.368 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski