Butyrivibrio sp. INlla14

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Butyrivibrio; unclassified Butyrivibrio

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3697 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5H9Y9|A0A1G5H9Y9_9FIRM Membrane protein implicated in regulation of membrane protease activity OS=Butyrivibrio sp. INlla14 OX=1520808 GN=SAMN02910371_02991 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 10.44KK3 pKa = 10.27KK4 pKa = 10.27IAVLLAGVMVCTMFTACGKK23 pKa = 10.13DD24 pKa = 3.27AGSDD28 pKa = 3.4AANEE32 pKa = 4.44AISVNEE38 pKa = 3.87EE39 pKa = 3.85ALASAAADD47 pKa = 3.31VSTEE51 pKa = 4.05GEE53 pKa = 4.32DD54 pKa = 3.16TGLTIDD60 pKa = 4.63FDD62 pKa = 4.17NLEE65 pKa = 4.25TSTLQDD71 pKa = 2.8IKK73 pKa = 11.33ASDD76 pKa = 3.91LVTLGEE82 pKa = 4.33YY83 pKa = 10.65NGITVEE89 pKa = 4.06ATLGEE94 pKa = 4.34VTEE97 pKa = 4.53EE98 pKa = 4.04DD99 pKa = 3.58VAGYY103 pKa = 9.47IEE105 pKa = 4.63NMKK108 pKa = 10.51SSNPPMIDD116 pKa = 3.03VTDD119 pKa = 3.99RR120 pKa = 11.84AVQDD124 pKa = 3.49GDD126 pKa = 4.11TVNIDD131 pKa = 3.63YY132 pKa = 10.54VGKK135 pKa = 10.53YY136 pKa = 10.47ADD138 pKa = 3.68TKK140 pKa = 10.45EE141 pKa = 4.28AFDD144 pKa = 4.2GGTANGADD152 pKa = 3.94LVIGSNSYY160 pKa = 10.54IEE162 pKa = 4.6GFEE165 pKa = 4.16SGLVGAEE172 pKa = 3.58IGEE175 pKa = 4.34TRR177 pKa = 11.84DD178 pKa = 3.75LNLTFPADD186 pKa = 3.58YY187 pKa = 10.24GAEE190 pKa = 4.04SLAGKK195 pKa = 10.11DD196 pKa = 3.24VVFTVTINSIKK207 pKa = 10.38VAADD211 pKa = 4.34DD212 pKa = 5.99ISDD215 pKa = 3.27EE216 pKa = 4.09WAAGLGIADD225 pKa = 3.58VTTISEE231 pKa = 4.27LEE233 pKa = 4.02AYY235 pKa = 9.85ANKK238 pKa = 9.44TLTTQAEE245 pKa = 4.31DD246 pKa = 3.96TYY248 pKa = 11.45KK249 pKa = 10.1STIEE253 pKa = 3.9NSAVQQVYY261 pKa = 10.34DD262 pKa = 3.47SCAFGDD268 pKa = 4.07IPQTLINRR276 pKa = 11.84YY277 pKa = 9.26LKK279 pKa = 10.4LQKK282 pKa = 10.65QMLDD286 pKa = 3.68YY287 pKa = 10.54QATMYY292 pKa = 10.38SYY294 pKa = 11.6YY295 pKa = 10.67YY296 pKa = 9.88GQQLSASDD304 pKa = 4.88LISVYY309 pKa = 10.09MNNEE313 pKa = 3.86GFVGTADD320 pKa = 5.56DD321 pKa = 4.24YY322 pKa = 11.5LQSISTDD329 pKa = 3.09MAKK332 pKa = 10.08QYY334 pKa = 11.68LMFQAIADD342 pKa = 3.99EE343 pKa = 4.07QGITISDD350 pKa = 3.83EE351 pKa = 5.77DD352 pKa = 3.87IDD354 pKa = 4.52TYY356 pKa = 11.62LKK358 pKa = 10.62DD359 pKa = 3.53AYY361 pKa = 9.68EE362 pKa = 4.36NASTTSFSSYY372 pKa = 10.65EE373 pKa = 4.02EE374 pKa = 4.18YY375 pKa = 10.47KK376 pKa = 11.0ASLDD380 pKa = 3.56LEE382 pKa = 4.7TYY384 pKa = 10.47RR385 pKa = 11.84EE386 pKa = 3.83GLMADD391 pKa = 3.5KK392 pKa = 10.67AVEE395 pKa = 4.67FIVNNANVVAAPVEE409 pKa = 4.4ATTEE413 pKa = 4.12TSAEE417 pKa = 4.05ASSEE421 pKa = 4.05ASVDD425 pKa = 3.35AAAEE429 pKa = 4.15SSSTVAEE436 pKa = 4.2

Molecular weight: 46.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5BRX8|A0A1G5BRX8_9FIRM SCP-2 sterol transfer family protein OS=Butyrivibrio sp. INlla14 OX=1520808 GN=SAMN02910371_00473 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 8.74MTFQPHH8 pKa = 5.93KK9 pKa = 8.68LQRR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36GGRR28 pKa = 11.84KK29 pKa = 8.97VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3697

0

3697

1242442

39

2640

336.1

37.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.288 ± 0.041

1.343 ± 0.015

6.517 ± 0.029

7.333 ± 0.042

4.357 ± 0.029

6.88 ± 0.04

1.625 ± 0.017

7.6 ± 0.038

7.096 ± 0.034

8.437 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.994 ± 0.019

4.79 ± 0.028

3.093 ± 0.022

2.815 ± 0.021

4.077 ± 0.029

6.234 ± 0.035

5.308 ± 0.038

6.815 ± 0.034

0.931 ± 0.013

4.467 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski