Beet pseudoyellows virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Crinivirus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6VRA5|Q6VRA5_9CLOS HSP70h OS=Beet pseudoyellows virus OX=72750 PE=3 SV=1
MM1 pKa = 7.44LCIFKK6 pKa = 10.41LRR8 pKa = 11.84QGGSFNVLYY17 pKa = 10.78LSDD20 pKa = 3.78SRR22 pKa = 11.84DD23 pKa = 3.5FSGEE27 pKa = 3.94TFLISSDD34 pKa = 3.83DD35 pKa = 3.66VTLLEE40 pKa = 5.01DD41 pKa = 4.76LMSHH45 pKa = 5.96LHH47 pKa = 7.0FIKK50 pKa = 9.52TQWW53 pKa = 2.86

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6VRA7|Q6VRA7_9CLOS ORF 2 OS=Beet pseudoyellows virus OX=72750 PE=4 SV=1
MM1 pKa = 7.45VINSGLVSGPTRR13 pKa = 11.84GGVIPTILGLFDD25 pKa = 3.77VPIRR29 pKa = 11.84PHH31 pKa = 6.46KK32 pKa = 10.5NIYY35 pKa = 9.28EE36 pKa = 3.79ISGFFNMLILCVLIFLFLNRR56 pKa = 11.84FLLRR60 pKa = 11.84FRR62 pKa = 11.84SNGYY66 pKa = 8.84VIRR69 pKa = 11.84DD70 pKa = 3.4NRR72 pKa = 11.84FDD74 pKa = 3.71SVV76 pKa = 3.12

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

4916

53

2441

491.6

56.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.007 ± 0.366

1.851 ± 0.23

6.448 ± 0.411

5.289 ± 0.358

5.899 ± 0.391

4.76 ± 0.285

1.566 ± 0.195

7.099 ± 0.262

7.445 ± 0.282

9.825 ± 0.444

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.421 ± 0.442

5.858 ± 0.66

3.214 ± 0.295

2.746 ± 0.352

5.207 ± 0.433

9.154 ± 0.481

5.696 ± 0.329

7.018 ± 0.399

0.529 ± 0.086

3.967 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski