Acinetobacter phage AM24

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 146 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0MH36|A0A1J0MH36_9CAUD Uncharacterized protein OS=Acinetobacter phage AM24 OX=1913571 PE=4 SV=1
MM1 pKa = 7.41SHH3 pKa = 7.75PDD5 pKa = 3.59YY6 pKa = 10.56EE7 pKa = 4.9SKK9 pKa = 9.87IHH11 pKa = 6.58KK12 pKa = 9.64IKK14 pKa = 10.97SGDD17 pKa = 3.32VGKK20 pKa = 10.83VVGIIPDD27 pKa = 4.27DD28 pKa = 4.5DD29 pKa = 4.56PSLVLLFLYY38 pKa = 10.25NPNWDD43 pKa = 3.55LDD45 pKa = 3.62EE46 pKa = 5.09YY47 pKa = 10.11YY48 pKa = 10.18PHH50 pKa = 8.08DD51 pKa = 4.62IGDD54 pKa = 3.95EE55 pKa = 4.55FIGDD59 pKa = 3.83PEE61 pKa = 4.32EE62 pKa = 4.3ACLVYY67 pKa = 10.33IRR69 pKa = 11.84EE70 pKa = 4.37CDD72 pKa = 3.08LHH74 pKa = 7.73GINEE78 pKa = 4.36SEE80 pKa = 4.44AKK82 pKa = 10.18LL83 pKa = 3.59

Molecular weight: 9.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0MGM0|A0A1J0MGM0_9CAUD Tail fiber protein OS=Acinetobacter phage AM24 OX=1913571 PE=4 SV=1
MM1 pKa = 7.49ARR3 pKa = 11.84GGLPEE8 pKa = 4.09WQKK11 pKa = 11.08ARR13 pKa = 11.84YY14 pKa = 6.18RR15 pKa = 11.84TTYY18 pKa = 10.19AGKK21 pKa = 9.07KK22 pKa = 9.86AYY24 pKa = 7.64TVRR27 pKa = 11.84HH28 pKa = 6.21KK29 pKa = 11.08NLTMHH34 pKa = 6.38VNTEE38 pKa = 3.71KK39 pKa = 11.3LEE41 pKa = 4.09ALKK44 pKa = 11.2GEE46 pKa = 4.31LVNLSLQHH54 pKa = 4.91VRR56 pKa = 11.84YY57 pKa = 10.07GIVDD61 pKa = 3.94DD62 pKa = 4.21NRR64 pKa = 11.84YY65 pKa = 6.19PTSDD69 pKa = 2.9PRR71 pKa = 11.84GRR73 pKa = 11.84GGMYY77 pKa = 10.13VATIWKK83 pKa = 8.86RR84 pKa = 11.84LEE86 pKa = 4.18FGSVDD91 pKa = 4.02EE92 pKa = 4.78INGKK96 pKa = 9.89ARR98 pKa = 11.84VIPPRR103 pKa = 11.84PTFTQHH109 pKa = 4.34VHH111 pKa = 5.58TGGKK115 pKa = 8.17GTAKK119 pKa = 10.27LVMMDD124 pKa = 3.3IMHH127 pKa = 7.36DD128 pKa = 3.63VFTGKK133 pKa = 9.27SVRR136 pKa = 11.84DD137 pKa = 3.73KK138 pKa = 11.41AWVKK142 pKa = 10.72LGLAMQSRR150 pKa = 11.84LKK152 pKa = 10.52FDD154 pKa = 3.42VMTQKK159 pKa = 10.98GFVPLKK165 pKa = 10.14LPSWSDD171 pKa = 3.27RR172 pKa = 11.84SPTDD176 pKa = 3.76FYY178 pKa = 11.52NDD180 pKa = 3.27TGHH183 pKa = 8.05LIRR186 pKa = 11.84SISYY190 pKa = 8.82RR191 pKa = 11.84IMPIGLDD198 pKa = 3.57FSKK201 pKa = 11.27

Molecular weight: 22.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

146

0

146

25900

37

848

177.4

20.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.815 ± 0.256

1.216 ± 0.102

6.486 ± 0.178

7.189 ± 0.259

4.151 ± 0.138

7.062 ± 0.196

1.865 ± 0.12

6.259 ± 0.182

7.672 ± 0.265

8.116 ± 0.195

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.625 ± 0.112

4.923 ± 0.185

3.089 ± 0.137

3.699 ± 0.12

4.394 ± 0.142

6.32 ± 0.221

5.795 ± 0.244

7.68 ± 0.18

1.378 ± 0.088

4.266 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski