Punica granatum (Pomegranate)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Myrtales; Lythraceae; Punica

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50426 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A218XTQ1|A0A218XTQ1_PUNGR uncharacterized protein LOC116215032 OS=Punica granatum OX=22663 GN=LOC116215032 PE=4 SV=1
MM1 pKa = 7.57ASSLVLEE8 pKa = 4.63SRR10 pKa = 11.84GIANNVKK17 pKa = 9.98LHH19 pKa = 6.67EE20 pKa = 4.9DD21 pKa = 3.51FEE23 pKa = 6.56GIDD26 pKa = 3.76VEE28 pKa = 4.34VLEE31 pKa = 4.95INSSILKK38 pKa = 10.42SLLEE42 pKa = 4.39DD43 pKa = 4.11AQGDD47 pKa = 4.07DD48 pKa = 4.31QDD50 pKa = 4.28YY51 pKa = 11.17DD52 pKa = 3.44QLNDD56 pKa = 3.5VIRR59 pKa = 11.84SLEE62 pKa = 3.93AEE64 pKa = 4.23IDD66 pKa = 3.75PSSLAGHH73 pKa = 7.37DD74 pKa = 4.25LGTHH78 pKa = 5.31TKK80 pKa = 10.33VINDD84 pKa = 4.04LVAGDD89 pKa = 4.25EE90 pKa = 4.06NSTINSRR97 pKa = 11.84GRR99 pKa = 11.84DD100 pKa = 3.54GSRR103 pKa = 11.84DD104 pKa = 3.0WSEE107 pKa = 3.91YY108 pKa = 10.51SSDD111 pKa = 3.54DD112 pKa = 4.09FSWIDD117 pKa = 3.42MDD119 pKa = 4.61TVPSSPGEE127 pKa = 4.0GMDD130 pKa = 2.81WYY132 pKa = 10.36MEE134 pKa = 4.14EE135 pKa = 5.55FGDD138 pKa = 5.23EE139 pKa = 4.49MNCLTQFGCIGDD151 pKa = 3.96YY152 pKa = 10.05YY153 pKa = 10.74QYY155 pKa = 9.6PQVYY159 pKa = 9.7SSEE162 pKa = 4.06EE163 pKa = 3.72PAYY166 pKa = 10.52GSLWHH171 pKa = 5.94EE172 pKa = 4.57TSHH175 pKa = 7.26EE176 pKa = 4.07MMEE179 pKa = 4.21

Molecular weight: 20.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I0HYL5|A0A2I0HYL5_PUNGR Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_042983 PE=4 SV=1
MM1 pKa = 8.03DD2 pKa = 4.85FASTLPFSTDD12 pKa = 3.33FGLVTLRR19 pKa = 11.84LSTGFGLVTLRR30 pKa = 11.84LSTGFGLVTLRR41 pKa = 11.84LSTGFGLVTLRR52 pKa = 11.84LSTGFGLVTLRR63 pKa = 11.84LSTGFGLVTLRR74 pKa = 11.84LSTGFGLVTLRR85 pKa = 11.84LSTGFGLVTLRR96 pKa = 11.84LSTGFGLVTLRR107 pKa = 11.84LSTGFGLVTLRR118 pKa = 11.84LSTGFGLVTLRR129 pKa = 11.84LSTGFGLVTLRR140 pKa = 11.84LSTGFGLVTLRR151 pKa = 11.84LSTGFGLVTLRR162 pKa = 11.84LSTGFGLVTLRR173 pKa = 11.84LSTGFGLVTLRR184 pKa = 11.84LSTGFGLVTLRR195 pKa = 11.84LSTGFGLVTLRR206 pKa = 11.84LSTGFGLVTLRR217 pKa = 11.84LSTGFGLVTLRR228 pKa = 11.84LSTGFGLVTLRR239 pKa = 11.84LSTGFGLVTLRR250 pKa = 11.84LSTGFGLVTLRR261 pKa = 11.84LSTGFGLVTLRR272 pKa = 11.84LSTGFGLVTLRR283 pKa = 11.84LSTGFGLVTLRR294 pKa = 11.84LSTGFGLVTLRR305 pKa = 11.84LSTGFGLVTLRR316 pKa = 11.84LSTGFGLVTLRR327 pKa = 11.84LSTGFGLVTLRR338 pKa = 11.84LSTGFGLVTLRR349 pKa = 11.84LSTGFGLVTLRR360 pKa = 11.84LSTGFGLVTLRR371 pKa = 11.84LSTGFGLVTLRR382 pKa = 11.84LSTGFGLVTLRR393 pKa = 11.84LSTGFGLVTLRR404 pKa = 11.84LSTGFGLVTLRR415 pKa = 11.84LSTGFGLVTLRR426 pKa = 11.84LSTGFGLVTLRR437 pKa = 11.84LSTGFGLVTLRR448 pKa = 11.84LSTGFGLVTLRR459 pKa = 11.84LSTGFGLVTLRR470 pKa = 11.84LSTGFGLVTLRR481 pKa = 11.84LSTGFGLVTLRR492 pKa = 11.84LSTGFGLVTLRR503 pKa = 11.84LSTGFGLVTLRR514 pKa = 11.84LSTGFGLVTLRR525 pKa = 11.84LSTGFGLVTLRR536 pKa = 11.84LSTGFGLVTLRR547 pKa = 11.84LSTGFGLVTLRR558 pKa = 11.84LSTGFGLVTLRR569 pKa = 11.84LSTGFGLVTLRR580 pKa = 11.84LSTGFGLVTLRR591 pKa = 11.84LSTGFGLVTLRR602 pKa = 11.84LSTGFGLVTLRR613 pKa = 11.84LSTGFGLVTLRR624 pKa = 11.84LSTGFGLVTLRR635 pKa = 11.84LSTGFGLVTLRR646 pKa = 11.84LSTGFGLVTLRR657 pKa = 11.84LSTGFGLVTLRR668 pKa = 11.84LSTGFGLVTLRR679 pKa = 11.84LSTGFGLVTLRR690 pKa = 11.84LSTGFGLVTLRR701 pKa = 11.84LSTGFGLVTLRR712 pKa = 11.84LSTGFGLVTLRR723 pKa = 11.84LSTGFGLVTLRR734 pKa = 11.84LSTGFGLVTLRR745 pKa = 11.84LSTGFGLVTLRR756 pKa = 11.84LSTGFGLVTLRR767 pKa = 11.84LSTGFGLVTLPFSTDD782 pKa = 3.4FGLVTLRR789 pKa = 11.84LSTGFGLVTLLLSEE803 pKa = 4.84LGVHH807 pKa = 6.68GLRR810 pKa = 11.84IHH812 pKa = 5.82FTVFHH817 pKa = 6.73GLRR820 pKa = 11.84PSNFTVFHH828 pKa = 6.6GLRR831 pKa = 11.84PGNITAFRR839 pKa = 11.84GLRR842 pKa = 11.84LSKK845 pKa = 10.42FIAFRR850 pKa = 11.84TSRR853 pKa = 11.84PWTSRR858 pKa = 11.84PLYY861 pKa = 9.64RR862 pKa = 11.84FPRR865 pKa = 11.84ASAA868 pKa = 3.27

Molecular weight: 91.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50426

0

50426

14680046

11

11234

291.1

32.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.176 ± 0.022

2.043 ± 0.015

4.916 ± 0.018

6.147 ± 0.023

4.216 ± 0.02

7.248 ± 0.031

2.463 ± 0.01

4.684 ± 0.017

5.168 ± 0.02

9.854 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.305 ± 0.009

3.592 ± 0.014

5.962 ± 0.021

3.345 ± 0.01

6.666 ± 0.022

8.957 ± 0.024

4.994 ± 0.013

6.35 ± 0.013

1.33 ± 0.007

2.578 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski