Mycolicibacterium chitae (Mycobacterium chitae)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales;

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A448HYH8|A0A448HYH8_MYCCI Acyl CoA:acetate/3-ketoacid CoA transferase subunit alpha OS=Mycolicibacterium chitae OX=1792 GN=gctA PE=4 SV=1
MM1 pKa = 7.28FSARR5 pKa = 11.84CSVALAALVAALVAVVGCGGGQAEE29 pKa = 4.7SVGLPDD35 pKa = 4.54PEE37 pKa = 4.23PEE39 pKa = 3.72RR40 pKa = 11.84TEE42 pKa = 3.9RR43 pKa = 11.84VVVPNVPTEE52 pKa = 3.96TGEE55 pKa = 4.04MFVVYY60 pKa = 9.8EE61 pKa = 4.14DD62 pKa = 4.24AEE64 pKa = 4.35SPEE67 pKa = 4.06AVRR70 pKa = 11.84GRR72 pKa = 11.84EE73 pKa = 3.99LLTEE77 pKa = 4.37ANLLEE82 pKa = 4.41EE83 pKa = 5.09LADD86 pKa = 4.24DD87 pKa = 4.22VNAMLLLPVDD97 pKa = 3.72VPVNGRR103 pKa = 11.84QCDD106 pKa = 3.3EE107 pKa = 4.54ANAYY111 pKa = 7.69WSPSEE116 pKa = 3.81QAMTICYY123 pKa = 9.67EE124 pKa = 3.99DD125 pKa = 4.39AEE127 pKa = 4.18LSEE130 pKa = 5.42RR131 pKa = 11.84IFEE134 pKa = 4.3EE135 pKa = 4.8AGEE138 pKa = 4.28EE139 pKa = 4.06DD140 pKa = 4.26PLAATLGAEE149 pKa = 3.9RR150 pKa = 11.84ATFYY154 pKa = 11.07HH155 pKa = 6.5EE156 pKa = 5.02LGHH159 pKa = 7.19AVIDD163 pKa = 4.85LYY165 pKa = 11.34DD166 pKa = 3.68LPFTGRR172 pKa = 11.84EE173 pKa = 3.74EE174 pKa = 4.79DD175 pKa = 4.27VADD178 pKa = 3.64QLAAVLLLGAGDD190 pKa = 5.43DD191 pKa = 4.1GTADD195 pKa = 3.74PEE197 pKa = 4.27NVEE200 pKa = 4.01AAQAYY205 pKa = 9.75ALMFQGYY212 pKa = 8.57SEE214 pKa = 4.16QDD216 pKa = 2.82GGGAEE221 pKa = 4.91EE222 pKa = 4.41FPFWDD227 pKa = 3.23VHH229 pKa = 6.11EE230 pKa = 4.69YY231 pKa = 11.31DD232 pKa = 3.98LARR235 pKa = 11.84MYY237 pKa = 11.12NFQCWIYY244 pKa = 11.14GSDD247 pKa = 3.69PEE249 pKa = 4.86NNAFMVDD256 pKa = 3.78DD257 pKa = 4.52GFVPDD262 pKa = 4.82EE263 pKa = 4.56RR264 pKa = 11.84ADD266 pKa = 3.59SCEE269 pKa = 4.18GEE271 pKa = 4.29FDD273 pKa = 3.29RR274 pKa = 11.84MSRR277 pKa = 11.84AWYY280 pKa = 10.18EE281 pKa = 3.52MLEE284 pKa = 3.81PHH286 pKa = 6.71LRR288 pKa = 11.84DD289 pKa = 3.32EE290 pKa = 4.51

Molecular weight: 31.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A448I0B2|A0A448I0B2_MYCCI Metalloendopeptidase-like membrane protein OS=Mycolicibacterium chitae OX=1792 GN=lytM_1 PE=4 SV=1
MM1 pKa = 7.53PPGWPRR7 pKa = 11.84RR8 pKa = 11.84WLTRR12 pKa = 11.84ALVLGTATRR21 pKa = 11.84AGRR24 pKa = 11.84GAVVPASAALPFVFRR39 pKa = 11.84GAVNTLAGG47 pKa = 3.77

Molecular weight: 4.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5110

0

5110

1670072

29

6531

326.8

35.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.254 ± 0.055

0.781 ± 0.01

6.372 ± 0.028

5.544 ± 0.029

3.05 ± 0.019

8.858 ± 0.033

2.124 ± 0.016

4.226 ± 0.022

2.13 ± 0.023

9.937 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.019 ± 0.014

2.192 ± 0.017

5.893 ± 0.033

2.999 ± 0.016

7.198 ± 0.031

5.129 ± 0.018

6.043 ± 0.022

8.667 ± 0.034

1.464 ± 0.014

2.122 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski