Sporolactobacillus inulinus CASD

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Sporolactobacillaceae; Sporolactobacillus; Sporolactobacillus inulinus

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2668 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1QQM6|A0A0U1QQM6_9BACL Riboflavin biosynthesis protein OS=Sporolactobacillus inulinus CASD OX=1069536 GN=SINU_04325 PE=3 SV=1
MM1 pKa = 7.61HH2 pKa = 6.74YY3 pKa = 10.81VIVDD7 pKa = 3.44KK8 pKa = 9.77DD9 pKa = 3.49TCIACGACGCAAPDD23 pKa = 3.21IFGYY27 pKa = 10.9DD28 pKa = 3.35DD29 pKa = 5.5DD30 pKa = 4.3GTSRR34 pKa = 11.84VLLDD38 pKa = 4.52DD39 pKa = 3.83NQGTKK44 pKa = 10.18EE45 pKa = 4.02IPEE48 pKa = 4.23AQLDD52 pKa = 4.07DD53 pKa = 5.49VIDD56 pKa = 3.97ACEE59 pKa = 4.21GCPTEE64 pKa = 4.4SIKK67 pKa = 10.6MSGTPFNGDD76 pKa = 3.4PAKK79 pKa = 10.45EE80 pKa = 3.91EE81 pKa = 4.49HH82 pKa = 6.46EE83 pKa = 4.32ASS85 pKa = 3.54

Molecular weight: 9.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1QSP4|A0A0U1QSP4_9BACL Amidase OS=Sporolactobacillus inulinus CASD OX=1069536 GN=SINU_00725 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.45GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 9.03VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2668

0

2668

789120

26

1841

295.8

33.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.137 ± 0.059

0.875 ± 0.017

5.325 ± 0.04

6.176 ± 0.06

4.374 ± 0.042

6.876 ± 0.048

2.397 ± 0.025

7.158 ± 0.042

6.469 ± 0.042

10.126 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.744 ± 0.024

3.958 ± 0.033

3.767 ± 0.027

4.101 ± 0.034

4.937 ± 0.041

6.041 ± 0.041

5.36 ± 0.034

6.925 ± 0.035

0.979 ± 0.017

3.275 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski