Beihai sphaeromadae virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KFI2|A0A1L3KFI2_9VIRU Capsid protein OS=Beihai sphaeromadae virus 3 OX=1922709 PE=4 SV=1
MM1 pKa = 8.01ANGKK5 pKa = 8.94QNNQRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84VPPNPNPPKK21 pKa = 9.85PRR23 pKa = 11.84ANRR26 pKa = 11.84APRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84NMRR34 pKa = 11.84PSNGNVTLVGANQALQRR51 pKa = 11.84YY52 pKa = 7.03NNPTGGHH59 pKa = 5.88RR60 pKa = 11.84QTGSDD65 pKa = 4.07LLTAITIQEE74 pKa = 4.24TVNAGEE80 pKa = 4.47LLADD84 pKa = 4.32IIITPSVAARR94 pKa = 11.84LEE96 pKa = 4.02VLAAAWQRR104 pKa = 11.84LRR106 pKa = 11.84YY107 pKa = 9.53NRR109 pKa = 11.84LTFEE113 pKa = 4.5VNASASSIVGGEE125 pKa = 3.68FVAAFVSDD133 pKa = 3.72PTDD136 pKa = 3.67RR137 pKa = 11.84PPLKK141 pKa = 10.84DD142 pKa = 2.91ADD144 pKa = 3.78KK145 pKa = 10.27WVKK148 pKa = 10.29AHH150 pKa = 6.85AGSITSSWWKK160 pKa = 10.14SMNVRR165 pKa = 11.84GPCPPQMMYY174 pKa = 10.62TSFEE178 pKa = 4.22EE179 pKa = 4.04NEE181 pKa = 4.17PRR183 pKa = 11.84FSSPGRR189 pKa = 11.84FVVAVVNPPTSAATMSISLSWDD211 pKa = 3.1VSFTQPSLEE220 pKa = 4.15TFIEE224 pKa = 4.51DD225 pKa = 4.13DD226 pKa = 3.6NNIYY230 pKa = 10.03IVNASSRR237 pKa = 11.84LPISDD242 pKa = 3.89GSAGQTFNPYY252 pKa = 10.23LIKK255 pKa = 10.73EE256 pKa = 4.22EE257 pKa = 4.31SDD259 pKa = 3.57GSFNDD264 pKa = 3.48NDD266 pKa = 5.22PITNYY271 pKa = 10.28LAATDD276 pKa = 4.25FTPEE280 pKa = 4.17LPDD283 pKa = 3.87GVLLQLPHH291 pKa = 7.28PKK293 pKa = 9.06TLNSDD298 pKa = 3.77TGASGAPEE306 pKa = 3.95NAIVTHH312 pKa = 6.55IGVADD317 pKa = 3.66GAGRR321 pKa = 11.84IGYY324 pKa = 7.37FLRR327 pKa = 11.84TEE329 pKa = 3.72ADD331 pKa = 3.65YY332 pKa = 10.7FQLLAGFPFSIRR344 pKa = 11.84PVVDD348 pKa = 4.68SINQAGTIYY357 pKa = 10.79DD358 pKa = 3.83GEE360 pKa = 4.45AEE362 pKa = 4.27NEE364 pKa = 4.4VATLAAPSRR373 pKa = 11.84GRR375 pKa = 11.84RR376 pKa = 11.84SLTSGPVLSRR386 pKa = 11.84SSKK389 pKa = 10.53GRR391 pKa = 11.84MLSLLNSKK399 pKa = 10.26RR400 pKa = 11.84FF401 pKa = 3.42

Molecular weight:
43.52 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KFI2|A0A1L3KFI2_9VIRU Capsid protein OS=Beihai sphaeromadae virus 3 OX=1922709 PE=4 SV=1
MM1 pKa = 7.55EE2 pKa = 5.39SNVAITVRR10 pKa = 11.84PNDD13 pKa = 3.24IGHH16 pKa = 6.51FFSLRR21 pKa = 11.84SVASNSLKK29 pKa = 10.59IGGICLLGVASYY41 pKa = 9.91KK42 pKa = 10.18ICTSGWLEE50 pKa = 3.91KK51 pKa = 10.63CVEE54 pKa = 4.29DD55 pKa = 3.86LRR57 pKa = 11.84HH58 pKa = 6.61RR59 pKa = 11.84IISRR63 pKa = 11.84NKK65 pKa = 8.95TVINDD70 pKa = 3.61AGLVRR75 pKa = 11.84ATVQKK80 pKa = 10.85KK81 pKa = 8.47MEE83 pKa = 4.31LVSTRR88 pKa = 11.84VRR90 pKa = 11.84KK91 pKa = 9.19GHH93 pKa = 5.17SHH95 pKa = 4.88STAAAEE101 pKa = 4.14RR102 pKa = 11.84NSATEE107 pKa = 3.72TMIEE111 pKa = 4.17VVTANGFEE119 pKa = 4.64PYY121 pKa = 10.12VISPSPRR128 pKa = 11.84EE129 pKa = 3.76SDD131 pKa = 3.13LDD133 pKa = 4.26GIRR136 pKa = 11.84HH137 pKa = 5.23FHH139 pKa = 6.36SLADD143 pKa = 3.6LRR145 pKa = 11.84QDD147 pKa = 3.55YY148 pKa = 11.16RR149 pKa = 11.84NDD151 pKa = 3.79PLTDD155 pKa = 2.94RR156 pKa = 11.84HH157 pKa = 6.92IIVMTDD163 pKa = 1.97VDD165 pKa = 4.86YY166 pKa = 11.07YY167 pKa = 11.9VNMHH171 pKa = 6.71SIISLGRR178 pKa = 11.84PILLYY183 pKa = 9.38TFQPRR188 pKa = 11.84VVSGPVIDD196 pKa = 4.64GFFTITEE203 pKa = 4.29NIIHH207 pKa = 5.74YY208 pKa = 9.53RR209 pKa = 11.84VNGGKK214 pKa = 9.43DD215 pKa = 3.32VKK217 pKa = 10.98HH218 pKa = 5.69PTWNYY223 pKa = 8.83NQDD226 pKa = 3.34TVFVRR231 pKa = 11.84DD232 pKa = 3.48PVTTFWATLRR242 pKa = 11.84QTLFDD247 pKa = 3.45VTGISWISAKK257 pKa = 7.19MHH259 pKa = 6.15RR260 pKa = 11.84AFGIGPCGQQLTICTIDD277 pKa = 3.47QFEE280 pKa = 4.59LSPNRR285 pKa = 11.84NIVSIVPFARR295 pKa = 11.84CRR297 pKa = 11.84DD298 pKa = 3.71NLLTATEE305 pKa = 4.38YY306 pKa = 9.66GTEE309 pKa = 4.22LSRR312 pKa = 11.84TTYY315 pKa = 7.59QQKK318 pKa = 10.66SGVPVMNAITYY329 pKa = 10.16IGDD332 pKa = 3.76GDD334 pKa = 4.12PLISLGEE341 pKa = 4.11EE342 pKa = 4.46GNCASVQLPLKK353 pKa = 10.26DD354 pKa = 4.1LEE356 pKa = 4.51SLRR359 pKa = 11.84TAYY362 pKa = 10.36HH363 pKa = 6.72LSKK366 pKa = 10.02TNNLSDD372 pKa = 3.38TVRR375 pKa = 11.84RR376 pKa = 11.84SKK378 pKa = 11.2RR379 pKa = 11.84NDD381 pKa = 2.82KK382 pKa = 9.98EE383 pKa = 3.43AAIIHH388 pKa = 6.32RR389 pKa = 11.84FLVSEE394 pKa = 4.53SEE396 pKa = 4.01LHH398 pKa = 5.73PVEE401 pKa = 4.09VHH403 pKa = 6.96KK404 pKa = 10.66PGQLARR410 pKa = 11.84HH411 pKa = 4.95YY412 pKa = 10.69QSVEE416 pKa = 3.73KK417 pKa = 10.99DD418 pKa = 3.07HH419 pKa = 7.3DD420 pKa = 4.7LDD422 pKa = 3.58PTEE425 pKa = 3.92QGKK428 pKa = 10.13EE429 pKa = 3.91YY430 pKa = 10.63ARR432 pKa = 11.84EE433 pKa = 3.96YY434 pKa = 11.32APGPLTQTAVFPSEE448 pKa = 4.37SISNEE453 pKa = 3.28RR454 pKa = 11.84ATIEE458 pKa = 3.93GRR460 pKa = 11.84INLPQAKK467 pKa = 9.38AKK469 pKa = 10.37AKK471 pKa = 10.23EE472 pKa = 4.33SVTPRR477 pKa = 11.84MRR479 pKa = 11.84RR480 pKa = 11.84IARR483 pKa = 11.84DD484 pKa = 3.64FVKK487 pKa = 10.66HH488 pKa = 5.86LVPEE492 pKa = 4.22VGIGHH497 pKa = 7.88PYY499 pKa = 9.64SVSHH503 pKa = 7.43VEE505 pKa = 4.11EE506 pKa = 4.32QQQKK510 pKa = 8.97PLQRR514 pKa = 11.84ARR516 pKa = 11.84NDD518 pKa = 3.01ANRR521 pKa = 11.84FHH523 pKa = 7.63DD524 pKa = 4.72AFDD527 pKa = 4.49MITKK531 pKa = 10.07AFQKK535 pKa = 10.51KK536 pKa = 7.59EE537 pKa = 3.78AYY539 pKa = 8.25NAPNHH544 pKa = 6.13PRR546 pKa = 11.84NISTVPHH553 pKa = 5.58GQNVKK558 pKa = 10.69LSGFTYY564 pKa = 10.53AFKK567 pKa = 10.51EE568 pKa = 4.13SHH570 pKa = 6.71LKK572 pKa = 8.42HH573 pKa = 6.02QEE575 pKa = 3.02WYY577 pKa = 8.86MPCNTPSKK585 pKa = 9.84IASVVQKK592 pKa = 10.39LAAEE596 pKa = 4.29SEE598 pKa = 4.45QLVEE602 pKa = 3.78TDD604 pKa = 3.24YY605 pKa = 11.78SRR607 pKa = 11.84FDD609 pKa = 3.3GTFLRR614 pKa = 11.84FMRR617 pKa = 11.84EE618 pKa = 3.71QVEE621 pKa = 4.22FASYY625 pKa = 10.12KK626 pKa = 9.32RR627 pKa = 11.84WVHH630 pKa = 6.25KK631 pKa = 10.4DD632 pKa = 3.27HH633 pKa = 6.69IQEE636 pKa = 4.19LTDD639 pKa = 3.94LLANEE644 pKa = 4.42VDD646 pKa = 3.94SKK648 pKa = 11.68AVTRR652 pKa = 11.84KK653 pKa = 9.35GLKK656 pKa = 8.96YY657 pKa = 10.61QPDD660 pKa = 4.03CTRR663 pKa = 11.84LSGSALTTDD672 pKa = 3.96GNSIANAFVSYY683 pKa = 10.39AANRR687 pKa = 11.84LDD689 pKa = 3.78GMNDD693 pKa = 2.72VEE695 pKa = 3.87AWQRR699 pKa = 11.84IGIVYY704 pKa = 10.33GDD706 pKa = 3.88DD707 pKa = 3.55GLRR710 pKa = 11.84DD711 pKa = 3.74GTVSDD716 pKa = 4.64DD717 pKa = 3.84RR718 pKa = 11.84LMSTASSLGFEE729 pKa = 4.26LKK731 pKa = 10.26IINRR735 pKa = 11.84ATRR738 pKa = 11.84GQPVSFLSRR747 pKa = 11.84IYY749 pKa = 10.77ADD751 pKa = 3.62PWSSPASVQTPSRR764 pKa = 11.84TLLKK768 pKa = 10.35IHH770 pKa = 6.09TTCDD774 pKa = 3.21TQSDD778 pKa = 3.88IEE780 pKa = 4.26EE781 pKa = 4.27VGWAKK786 pKa = 8.65TQAYY790 pKa = 9.84LVTDD794 pKa = 4.46CLTPFISNWCKK805 pKa = 10.29AYY807 pKa = 10.46QRR809 pKa = 11.84NCTAKK814 pKa = 10.24VINYY818 pKa = 9.67KK819 pKa = 10.5DD820 pKa = 3.79FNDD823 pKa = 3.24IPFWVRR829 pKa = 11.84DD830 pKa = 3.19EE831 pKa = 5.68DD832 pKa = 4.0ALQNSWPQDD841 pKa = 3.54NSEE844 pKa = 3.49VWYY847 pKa = 9.98DD848 pKa = 4.27IVAQDD853 pKa = 3.96LGVSVGEE860 pKa = 3.96LHH862 pKa = 7.11RR863 pKa = 11.84HH864 pKa = 5.43LKK866 pKa = 10.77LLDD869 pKa = 3.93SYY871 pKa = 11.52NGPIKK876 pKa = 10.21DD877 pKa = 4.18LPRR880 pKa = 11.84LATALKK886 pKa = 7.6TQPKK890 pKa = 9.56LAVAMDD896 pKa = 4.14GEE898 pKa = 4.59IHH900 pKa = 7.47AGPTEE905 pKa = 4.09QNGQNPTSDD914 pKa = 3.31QPASDD919 pKa = 4.26GATKK923 pKa = 10.55ALPAVGGGKK932 pKa = 8.68NQSGRR937 pKa = 11.84TVSVRR942 pKa = 11.84HH943 pKa = 5.43QRR945 pKa = 11.84NAHH948 pKa = 6.24LRR950 pKa = 11.84DD951 pKa = 3.5QRR953 pKa = 11.84PGSNNKK959 pKa = 8.18TAGPGHH965 pKa = 7.1KK966 pKa = 9.52ISTPNGGTSQSSARR980 pKa = 11.84TNCAAGRR987 pKa = 11.84RR988 pKa = 11.84SAPQVGRR995 pKa = 11.84KK996 pKa = 6.65KK997 pKa = 8.76TRR999 pKa = 11.84KK1000 pKa = 9.28AA1001 pKa = 3.15

Molecular weight:
111.79 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1402

401

1001

701.0

77.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.131 ± 0.814

1.07 ± 0.419

5.777 ± 0.403

4.85 ± 0.184

3.352 ± 0.326

6.419 ± 0.287

2.71 ± 0.874

5.421 ± 0.221

4.494 ± 1.021

7.204 ± 0.142

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.498 ± 0.126

5.777 ± 0.87

5.991 ± 1.142

4.208 ± 0.493

6.99 ± 0.123

8.345 ± 0.577

6.919 ± 0.222

6.776 ± 0.276

1.284 ± 0.019

2.782 ± 0.274

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski