Botrytis cinerea hypovirus 1 satellite-like RNA

Taxonomy: Viruses; Satellites; RNA satellites

Average proteome isoelectric point is 5.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U9NJB8|A0A2U9NJB8_9VIRU Uncharacterized protein OS=Botrytis cinerea hypovirus 1 satellite-like RNA OX=2219109 PE=4 SV=1
MM1 pKa = 7.81NIKK4 pKa = 10.16NKK6 pKa = 10.48NNFLDD11 pKa = 3.64NSSRR15 pKa = 11.84SLASTSSARR24 pKa = 11.84DD25 pKa = 3.33AKK27 pKa = 10.35PDD29 pKa = 3.67RR30 pKa = 11.84EE31 pKa = 4.23HH32 pKa = 7.52PEE34 pKa = 3.48QKK36 pKa = 10.37LRR38 pKa = 11.84LEE40 pKa = 4.3VQRR43 pKa = 11.84AEE45 pKa = 4.29KK46 pKa = 10.42PPVRR50 pKa = 11.84PSVFAGTEE58 pKa = 3.84HH59 pKa = 6.58QEE61 pKa = 3.91QDD63 pKa = 3.1GTQHH67 pKa = 5.59VRR69 pKa = 11.84RR70 pKa = 11.84EE71 pKa = 3.89HH72 pKa = 6.45HH73 pKa = 5.9GAHH76 pKa = 5.87SRR78 pKa = 11.84EE79 pKa = 3.89HH80 pKa = 6.59SKK82 pKa = 10.74EE83 pKa = 3.74SRR85 pKa = 11.84AGRR88 pKa = 11.84SSSHH92 pKa = 5.54SRR94 pKa = 11.84RR95 pKa = 11.84HH96 pKa = 5.96DD97 pKa = 3.86SEE99 pKa = 3.84HH100 pKa = 6.46HH101 pKa = 5.0RR102 pKa = 11.84TPRR105 pKa = 11.84ASSRR109 pKa = 11.84AEE111 pKa = 3.55SRR113 pKa = 11.84SRR115 pKa = 11.84IEE117 pKa = 4.41GKK119 pKa = 8.01TAADD123 pKa = 3.81KK124 pKa = 10.63GFCYY128 pKa = 10.07AALFLRR134 pKa = 11.84EE135 pKa = 4.24KK136 pKa = 10.02RR137 pKa = 11.84DD138 pKa = 3.43EE139 pKa = 4.4VIRR142 pKa = 11.84AIATDD147 pKa = 3.97YY148 pKa = 10.42PQLNKK153 pKa = 9.47VRR155 pKa = 11.84RR156 pKa = 11.84FADD159 pKa = 3.24THH161 pKa = 5.8KK162 pKa = 10.67AWVKK166 pKa = 10.46DD167 pKa = 3.04GHH169 pKa = 5.95ITVTAAIGFHH179 pKa = 6.67AGPNGEE185 pKa = 4.62LGNLKK190 pKa = 9.75PLSIHH195 pKa = 4.99VTSGGDD201 pKa = 3.27VSIKK205 pKa = 10.48EE206 pKa = 4.3FVASDD211 pKa = 3.46SYY213 pKa = 11.89GSVKK217 pKa = 10.18IGGYY221 pKa = 10.32ADD223 pKa = 4.09EE224 pKa = 5.4EE225 pKa = 4.25EE226 pKa = 5.26LDD228 pKa = 5.96DD229 pKa = 4.31EE230 pKa = 5.11GAWEE234 pKa = 4.94DD235 pKa = 5.18EE236 pKa = 4.18DD237 pKa = 6.29DD238 pKa = 3.83GWEE241 pKa = 4.26DD242 pKa = 4.6DD243 pKa = 3.97EE244 pKa = 4.44PTPVVKK250 pKa = 10.5SSKK253 pKa = 9.45TVVSSYY259 pKa = 10.66KK260 pKa = 10.05ISPTSGGNLNWPSSSFHH277 pKa = 7.89SNMGSSSRR285 pKa = 11.84STPVTPVAQSLDD297 pKa = 3.15WSHH300 pKa = 6.76YY301 pKa = 9.21GLLHH305 pKa = 5.34QVEE308 pKa = 4.84PRR310 pKa = 11.84PIINPPLPVKK320 pKa = 10.72SPEE323 pKa = 3.89RR324 pKa = 11.84PQNISNSLDD333 pKa = 3.73FYY335 pKa = 11.37HH336 pKa = 7.21EE337 pKa = 4.37SSDD340 pKa = 3.68DD341 pKa = 3.33KK342 pKa = 11.49CYY344 pKa = 11.16DD345 pKa = 4.15EE346 pKa = 6.47LPDD349 pKa = 4.92DD350 pKa = 4.05YY351 pKa = 11.55VFEE354 pKa = 4.84EE355 pKa = 5.22GEE357 pKa = 4.18DD358 pKa = 3.34WGTLTWPLPSTGVVAAAMDD377 pKa = 3.8ATLDD381 pKa = 3.9TEE383 pKa = 4.32PLSAEE388 pKa = 3.6ISSPLEE394 pKa = 3.73KK395 pKa = 10.15TISDD399 pKa = 3.63AVNAVIDD406 pKa = 4.36DD407 pKa = 4.28VVDD410 pKa = 3.61SADD413 pKa = 3.45PVFAALEE420 pKa = 4.12AATQFRR426 pKa = 11.84PVASRR431 pKa = 11.84DD432 pKa = 3.25ITSLVPKK439 pKa = 10.6AVGTCLSALASGNQPVVVARR459 pKa = 11.84SGSTGIVGFINGLIEE474 pKa = 4.12VYY476 pKa = 9.11KK477 pKa = 9.76TIIVACDD484 pKa = 3.27NFDD487 pKa = 2.92KK488 pKa = 11.1SMRR491 pKa = 11.84VHH493 pKa = 6.86PGWNVVTSAEE503 pKa = 4.34HH504 pKa = 5.48IVNGRR509 pKa = 11.84VNLGEE514 pKa = 3.96TRR516 pKa = 11.84DD517 pKa = 3.85LGSLPEE523 pKa = 3.95IVKK526 pKa = 10.46GSFIVVVVDD535 pKa = 3.68FDD537 pKa = 3.03NWTVKK542 pKa = 10.26KK543 pKa = 10.08QALFSAFDD551 pKa = 3.54GKK553 pKa = 10.42IIRR556 pKa = 11.84TSSSLKK562 pKa = 10.25LRR564 pKa = 11.84STGKK568 pKa = 9.95RR569 pKa = 11.84VLIRR573 pKa = 11.84IPRR576 pKa = 11.84EE577 pKa = 3.94DD578 pKa = 3.73KK579 pKa = 10.13SCQIVVTKK587 pKa = 10.37DD588 pKa = 3.22YY589 pKa = 11.15SCVVQAGRR597 pKa = 11.84NGVFIFATDD606 pKa = 3.45HH607 pKa = 6.58DD608 pKa = 4.89VSDD611 pKa = 3.27KK612 pKa = 10.8FYY614 pKa = 10.29WYY616 pKa = 9.57ILKK619 pKa = 9.31ATVFPSWVKK628 pKa = 9.14RR629 pKa = 11.84WGVVQHH635 pKa = 6.52WDD637 pKa = 3.19MSYY640 pKa = 9.17LTPRR644 pKa = 11.84MWNNCLCDD652 pKa = 3.35VHH654 pKa = 8.74LGMQEE659 pKa = 3.59PALEE663 pKa = 4.44VNCFHH668 pKa = 7.39SVV670 pKa = 2.75

Molecular weight: 74.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U9NJB8|A0A2U9NJB8_9VIRU Uncharacterized protein OS=Botrytis cinerea hypovirus 1 satellite-like RNA OX=2219109 PE=4 SV=1
MM1 pKa = 7.81NIKK4 pKa = 10.16NKK6 pKa = 10.48NNFLDD11 pKa = 3.64NSSRR15 pKa = 11.84SLASTSSARR24 pKa = 11.84DD25 pKa = 3.33AKK27 pKa = 10.35PDD29 pKa = 3.67RR30 pKa = 11.84EE31 pKa = 4.23HH32 pKa = 7.52PEE34 pKa = 3.48QKK36 pKa = 10.37LRR38 pKa = 11.84LEE40 pKa = 4.3VQRR43 pKa = 11.84AEE45 pKa = 4.29KK46 pKa = 10.42PPVRR50 pKa = 11.84PSVFAGTEE58 pKa = 3.84HH59 pKa = 6.58QEE61 pKa = 3.91QDD63 pKa = 3.1GTQHH67 pKa = 5.59VRR69 pKa = 11.84RR70 pKa = 11.84EE71 pKa = 3.89HH72 pKa = 6.45HH73 pKa = 5.9GAHH76 pKa = 5.87SRR78 pKa = 11.84EE79 pKa = 3.89HH80 pKa = 6.59SKK82 pKa = 10.74EE83 pKa = 3.74SRR85 pKa = 11.84AGRR88 pKa = 11.84SSSHH92 pKa = 5.54SRR94 pKa = 11.84RR95 pKa = 11.84HH96 pKa = 5.96DD97 pKa = 3.86SEE99 pKa = 3.84HH100 pKa = 6.46HH101 pKa = 5.0RR102 pKa = 11.84TPRR105 pKa = 11.84ASSRR109 pKa = 11.84AEE111 pKa = 3.55SRR113 pKa = 11.84SRR115 pKa = 11.84IEE117 pKa = 4.41GKK119 pKa = 8.01TAADD123 pKa = 3.81KK124 pKa = 10.63GFCYY128 pKa = 10.07AALFLRR134 pKa = 11.84EE135 pKa = 4.24KK136 pKa = 10.02RR137 pKa = 11.84DD138 pKa = 3.43EE139 pKa = 4.4VIRR142 pKa = 11.84AIATDD147 pKa = 3.97YY148 pKa = 10.42PQLNKK153 pKa = 9.47VRR155 pKa = 11.84RR156 pKa = 11.84FADD159 pKa = 3.24THH161 pKa = 5.8KK162 pKa = 10.67AWVKK166 pKa = 10.46DD167 pKa = 3.04GHH169 pKa = 5.95ITVTAAIGFHH179 pKa = 6.67AGPNGEE185 pKa = 4.62LGNLKK190 pKa = 9.75PLSIHH195 pKa = 4.99VTSGGDD201 pKa = 3.27VSIKK205 pKa = 10.48EE206 pKa = 4.3FVASDD211 pKa = 3.46SYY213 pKa = 11.89GSVKK217 pKa = 10.18IGGYY221 pKa = 10.32ADD223 pKa = 4.09EE224 pKa = 5.4EE225 pKa = 4.25EE226 pKa = 5.26LDD228 pKa = 5.96DD229 pKa = 4.31EE230 pKa = 5.11GAWEE234 pKa = 4.94DD235 pKa = 5.18EE236 pKa = 4.18DD237 pKa = 6.29DD238 pKa = 3.83GWEE241 pKa = 4.26DD242 pKa = 4.6DD243 pKa = 3.97EE244 pKa = 4.44PTPVVKK250 pKa = 10.5SSKK253 pKa = 9.45TVVSSYY259 pKa = 10.66KK260 pKa = 10.05ISPTSGGNLNWPSSSFHH277 pKa = 7.89SNMGSSSRR285 pKa = 11.84STPVTPVAQSLDD297 pKa = 3.15WSHH300 pKa = 6.76YY301 pKa = 9.21GLLHH305 pKa = 5.34QVEE308 pKa = 4.84PRR310 pKa = 11.84PIINPPLPVKK320 pKa = 10.72SPEE323 pKa = 3.89RR324 pKa = 11.84PQNISNSLDD333 pKa = 3.73FYY335 pKa = 11.37HH336 pKa = 7.21EE337 pKa = 4.37SSDD340 pKa = 3.68DD341 pKa = 3.33KK342 pKa = 11.49CYY344 pKa = 11.16DD345 pKa = 4.15EE346 pKa = 6.47LPDD349 pKa = 4.92DD350 pKa = 4.05YY351 pKa = 11.55VFEE354 pKa = 4.84EE355 pKa = 5.22GEE357 pKa = 4.18DD358 pKa = 3.34WGTLTWPLPSTGVVAAAMDD377 pKa = 3.8ATLDD381 pKa = 3.9TEE383 pKa = 4.32PLSAEE388 pKa = 3.6ISSPLEE394 pKa = 3.73KK395 pKa = 10.15TISDD399 pKa = 3.63AVNAVIDD406 pKa = 4.36DD407 pKa = 4.28VVDD410 pKa = 3.61SADD413 pKa = 3.45PVFAALEE420 pKa = 4.12AATQFRR426 pKa = 11.84PVASRR431 pKa = 11.84DD432 pKa = 3.25ITSLVPKK439 pKa = 10.6AVGTCLSALASGNQPVVVARR459 pKa = 11.84SGSTGIVGFINGLIEE474 pKa = 4.12VYY476 pKa = 9.11KK477 pKa = 9.76TIIVACDD484 pKa = 3.27NFDD487 pKa = 2.92KK488 pKa = 11.1SMRR491 pKa = 11.84VHH493 pKa = 6.86PGWNVVTSAEE503 pKa = 4.34HH504 pKa = 5.48IVNGRR509 pKa = 11.84VNLGEE514 pKa = 3.96TRR516 pKa = 11.84DD517 pKa = 3.85LGSLPEE523 pKa = 3.95IVKK526 pKa = 10.46GSFIVVVVDD535 pKa = 3.68FDD537 pKa = 3.03NWTVKK542 pKa = 10.26KK543 pKa = 10.08QALFSAFDD551 pKa = 3.54GKK553 pKa = 10.42IIRR556 pKa = 11.84TSSSLKK562 pKa = 10.25LRR564 pKa = 11.84STGKK568 pKa = 9.95RR569 pKa = 11.84VLIRR573 pKa = 11.84IPRR576 pKa = 11.84EE577 pKa = 3.94DD578 pKa = 3.73KK579 pKa = 10.13SCQIVVTKK587 pKa = 10.37DD588 pKa = 3.22YY589 pKa = 11.15SCVVQAGRR597 pKa = 11.84NGVFIFATDD606 pKa = 3.45HH607 pKa = 6.58DD608 pKa = 4.89VSDD611 pKa = 3.27KK612 pKa = 10.8FYY614 pKa = 10.29WYY616 pKa = 9.57ILKK619 pKa = 9.31ATVFPSWVKK628 pKa = 9.14RR629 pKa = 11.84WGVVQHH635 pKa = 6.52WDD637 pKa = 3.19MSYY640 pKa = 9.17LTPRR644 pKa = 11.84MWNNCLCDD652 pKa = 3.35VHH654 pKa = 8.74LGMQEE659 pKa = 3.59PALEE663 pKa = 4.44VNCFHH668 pKa = 7.39SVV670 pKa = 2.75

Molecular weight: 74.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

670

670

670

670.0

74.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.313 ± 0.0

1.343 ± 0.0

7.313 ± 0.0

6.269 ± 0.0

3.433 ± 0.0

6.269 ± 0.0

3.731 ± 0.0

4.776 ± 0.0

5.224 ± 0.0

5.97 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.045 ± 0.0

3.881 ± 0.0

5.522 ± 0.0

2.388 ± 0.0

5.97 ± 0.0

10.896 ± 0.0

5.224 ± 0.0

9.254 ± 0.0

2.09 ± 0.0

2.09 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski