Amino acid dipepetide frequency for Branchiostoma floridae (Florida lancelet) (Amphioxus)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.376AlaAla: 6.376 ± 0.039	1.456AlaCys: 1.456 ± 0.02	3.833AlaAsp: 3.833 ± 0.022	4.424AlaGlu: 4.424 ± 0.03	2.429AlaPhe: 2.429 ± 0.018	4.846AlaGly: 4.846 ± 0.03	1.615AlaHis: 1.615 ± 0.016	3.203AlaIle: 3.203 ± 0.02	3.353AlaLys: 3.353 ± 0.025	5.908AlaLeu: 5.908 ± 0.042	1.846AlaMet: 1.846 ± 0.014	2.383AlaAsn: 2.383 ± 0.015	3.165AlaPro: 3.165 ± 0.028	2.653AlaGln: 2.653 ± 0.017	3.645AlaArg: 3.645 ± 0.023	4.898AlaSer: 4.898 ± 0.029	4.17AlaThr: 4.17 ± 0.023	5.426AlaVal: 5.426 ± 0.027	0.87AlaTrp: 0.87 ± 0.01	1.665AlaTyr: 1.665 ± 0.013	0.001AlaXaa: 0.001 ± 0.0
Cys	1.411CysAla: 1.411 ± 0.016	0.7CysCys: 0.7 ± 0.017	1.96CysAsp: 1.96 ± 0.044	1.609CysGlu: 1.609 ± 0.024	0.739CysPhe: 0.739 ± 0.011	2.086CysGly: 2.086 ± 0.035	0.681CysHis: 0.681 ± 0.016	0.914CysIle: 0.914 ± 0.012	1.085CysLys: 1.085 ± 0.013	1.989CysLeu: 1.989 ± 0.016	0.497CysMet: 0.497 ± 0.007	1.012CysAsn: 1.012 ± 0.014	1.403CysPro: 1.403 ± 0.021	1.179CysGln: 1.179 ± 0.019	1.401CysArg: 1.401 ± 0.015	2.107CysSer: 2.107 ± 0.023	1.617CysThr: 1.617 ± 0.027	1.565CysVal: 1.565 ± 0.017	0.321CysTrp: 0.321 ± 0.007	0.715CysTyr: 0.715 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	3.411AspAla: 3.411 ± 0.024	1.39AspCys: 1.39 ± 0.032	4.095AspAsp: 4.095 ± 0.066	3.789AspGlu: 3.789 ± 0.03	2.258AspPhe: 2.258 ± 0.018	4.553AspGly: 4.553 ± 0.032	1.469AspHis: 1.469 ± 0.015	3.184AspIle: 3.184 ± 0.023	2.955AspLys: 2.955 ± 0.022	4.925AspLeu: 4.925 ± 0.027	1.535AspMet: 1.535 ± 0.013	2.481AspAsn: 2.481 ± 0.021	2.878AspPro: 2.878 ± 0.018	2.408AspGln: 2.408 ± 0.019	3.116AspArg: 3.116 ± 0.021	4.079AspSer: 4.079 ± 0.028	3.345AspThr: 3.345 ± 0.033	4.386AspVal: 4.386 ± 0.029	0.81AspTrp: 0.81 ± 0.017	1.887AspTyr: 1.887 ± 0.018	0.001AspXaa: 0.001 ± 0.0
Glu	4.257GluAla: 4.257 ± 0.032	1.651GluCys: 1.651 ± 0.027	4.476GluAsp: 4.476 ± 0.026	6.423GluGlu: 6.423 ± 0.052	1.919GluPhe: 1.919 ± 0.015	4.136GluGly: 4.136 ± 0.027	1.45GluHis: 1.45 ± 0.013	2.699GluIle: 2.699 ± 0.017	4.514GluLys: 4.514 ± 0.041	4.95GluLeu: 4.95 ± 0.032	1.612GluMet: 1.612 ± 0.014	2.717GluAsn: 2.717 ± 0.022	2.476GluPro: 2.476 ± 0.026	2.715GluGln: 2.715 ± 0.021	3.528GluArg: 3.528 ± 0.024	3.656GluSer: 3.656 ± 0.026	3.742GluThr: 3.742 ± 0.03	4.177GluVal: 4.177 ± 0.022	0.733GluTrp: 0.733 ± 0.009	1.649GluTyr: 1.649 ± 0.013	0.001GluXaa: 0.001 ± 0.0
Phe	2.165PheAla: 2.165 ± 0.015	0.914PheCys: 0.914 ± 0.019	1.99PheAsp: 1.99 ± 0.016	1.742PheGlu: 1.742 ± 0.015	1.424PhePhe: 1.424 ± 0.013	2.468PheGly: 2.468 ± 0.019	0.988PheHis: 0.988 ± 0.012	1.674PheIle: 1.674 ± 0.018	1.622PheLys: 1.622 ± 0.013	3.508PheLeu: 3.508 ± 0.025	0.806PheMet: 0.806 ± 0.008	1.42PheAsn: 1.42 ± 0.012	1.832PhePro: 1.832 ± 0.015	1.51PheGln: 1.51 ± 0.012	1.962PheArg: 1.962 ± 0.016	3.078PheSer: 3.078 ± 0.022	2.26PheThr: 2.26 ± 0.016	2.462PheVal: 2.462 ± 0.018	0.509PheTrp: 0.509 ± 0.007	1.202PheTyr: 1.202 ± 0.012	0.0PheXaa: 0.0 ± 0.0
Gly	4.109GlyAla: 4.109 ± 0.028	1.408GlyCys: 1.408 ± 0.017	4.189GlyAsp: 4.189 ± 0.027	4.745GlyGlu: 4.745 ± 0.042	2.458GlyPhe: 2.458 ± 0.019	5.317GlyGly: 5.317 ± 0.039	1.838GlyHis: 1.838 ± 0.017	2.837GlyIle: 2.837 ± 0.018	3.77GlyLys: 3.77 ± 0.026	5.236GlyLeu: 5.236 ± 0.026	1.546GlyMet: 1.546 ± 0.013	3.068GlyAsn: 3.068 ± 0.023	2.98GlyPro: 2.98 ± 0.037	3.071GlyGln: 3.071 ± 0.023	4.014GlyArg: 4.014 ± 0.024	5.174GlySer: 5.174 ± 0.031	4.5GlyThr: 4.5 ± 0.036	4.436GlyVal: 4.436 ± 0.028	1.051GlyTrp: 1.051 ± 0.014	2.49GlyTyr: 2.49 ± 0.022	0.001GlyXaa: 0.001 ± 0.0
His	1.46HisAla: 1.46 ± 0.016	0.692HisCys: 0.692 ± 0.01	1.325HisAsp: 1.325 ± 0.017	1.199HisGlu: 1.199 ± 0.013	0.94HisPhe: 0.94 ± 0.009	1.823HisGly: 1.823 ± 0.017	0.912HisHis: 0.912 ± 0.012	1.271HisIle: 1.271 ± 0.013	1.228HisLys: 1.228 ± 0.015	2.702HisLeu: 2.702 ± 0.024	1.047HisMet: 1.047 ± 0.022	1.029HisAsn: 1.029 ± 0.011	1.621HisPro: 1.621 ± 0.015	1.119HisGln: 1.119 ± 0.013	1.629HisArg: 1.629 ± 0.014	1.797HisSer: 1.797 ± 0.018	2.114HisThr: 2.114 ± 0.032	1.85HisVal: 1.85 ± 0.017	0.337HisTrp: 0.337 ± 0.008	0.805HisTyr: 0.805 ± 0.01	0.0HisXaa: 0.0 ± 0.0
Ile	3.068IleAla: 3.068 ± 0.022	1.09IleCys: 1.09 ± 0.012	2.337IleAsp: 2.337 ± 0.024	2.259IleGlu: 2.259 ± 0.016	1.703IlePhe: 1.703 ± 0.015	2.622IleGly: 2.622 ± 0.019	1.254IleHis: 1.254 ± 0.013	2.113IleIle: 2.113 ± 0.016	2.139IleLys: 2.139 ± 0.015	3.959IleLeu: 3.959 ± 0.024	1.009IleMet: 1.009 ± 0.01	1.735IleAsn: 1.735 ± 0.014	2.522IlePro: 2.522 ± 0.02	1.974IleGln: 1.974 ± 0.014	2.462IleArg: 2.462 ± 0.016	3.326IleSer: 3.326 ± 0.027	2.906IleThr: 2.906 ± 0.023	2.933IleVal: 2.933 ± 0.021	0.52IleTrp: 0.52 ± 0.008	1.4IleTyr: 1.4 ± 0.015	0.001IleXaa: 0.001 ± 0.0
Lys	3.68LysAla: 3.68 ± 0.028	1.386LysCys: 1.386 ± 0.021	3.13LysAsp: 3.13 ± 0.023	3.953LysGlu: 3.953 ± 0.033	1.667LysPhe: 1.667 ± 0.013	3.01LysGly: 3.01 ± 0.024	1.578LysHis: 1.578 ± 0.02	2.23LysIle: 2.23 ± 0.023	3.882LysLys: 3.882 ± 0.033	4.485LysLeu: 4.485 ± 0.028	1.385LysMet: 1.385 ± 0.011	1.923LysAsn: 1.923 ± 0.015	3.126LysPro: 3.126 ± 0.035	2.244LysGln: 2.244 ± 0.019	3.196LysArg: 3.196 ± 0.022	3.494LysSer: 3.494 ± 0.027	3.325LysThr: 3.325 ± 0.021	3.429LysVal: 3.429 ± 0.02	0.602LysTrp: 0.602 ± 0.007	1.58LysTyr: 1.58 ± 0.015	0.001LysXaa: 0.001 ± 0.0
Leu	5.874LeuAla: 5.874 ± 0.029	1.946LeuCys: 1.946 ± 0.032	4.765LeuAsp: 4.765 ± 0.027	5.331LeuGlu: 5.331 ± 0.036	3.002LeuPhe: 3.002 ± 0.023	5.376LeuGly: 5.376 ± 0.035	2.414LeuHis: 2.414 ± 0.017	3.162LeuIle: 3.162 ± 0.021	4.983LeuLys: 4.983 ± 0.034	8.001LeuLeu: 8.001 ± 0.047	1.962LeuMet: 1.962 ± 0.017	3.23LeuAsn: 3.23 ± 0.021	4.936LeuPro: 4.936 ± 0.028	4.848LeuGln: 4.848 ± 0.031	5.045LeuArg: 5.045 ± 0.028	6.799LeuSer: 6.799 ± 0.035	5.286LeuThr: 5.286 ± 0.027	5.598LeuVal: 5.598 ± 0.031	1.032LeuTrp: 1.032 ± 0.01	2.693LeuTyr: 2.693 ± 0.018	0.002LeuXaa: 0.002 ± 0.0
Met	2.263MetAla: 2.263 ± 0.017	0.685MetCys: 0.685 ± 0.013	1.391MetAsp: 1.391 ± 0.012	1.753MetGlu: 1.753 ± 0.012	0.951MetPhe: 0.951 ± 0.01	1.438MetGly: 1.438 ± 0.014	0.465MetHis: 0.465 ± 0.006	0.888MetIle: 0.888 ± 0.009	1.401MetLys: 1.401 ± 0.012	1.904MetLeu: 1.904 ± 0.016	0.698MetMet: 0.698 ± 0.01	0.818MetAsn: 0.818 ± 0.009	1.14MetPro: 1.14 ± 0.011	0.92MetGln: 0.92 ± 0.009	1.54MetArg: 1.54 ± 0.02	1.79MetSer: 1.79 ± 0.012	1.569MetThr: 1.569 ± 0.013	1.594MetVal: 1.594 ± 0.01	0.306MetTrp: 0.306 ± 0.005	0.828MetTyr: 0.828 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.281AsnAla: 2.281 ± 0.014	1.017AsnCys: 1.017 ± 0.015	2.051AsnAsp: 2.051 ± 0.017	1.976AsnGlu: 1.976 ± 0.016	1.444AsnPhe: 1.444 ± 0.013	2.899AsnGly: 2.899 ± 0.024	1.007AsnHis: 1.007 ± 0.013	2.296AsnIle: 2.296 ± 0.016	2.094AsnLys: 2.094 ± 0.017	3.773AsnLeu: 3.773 ± 0.025	1.142AsnMet: 1.142 ± 0.011	2.152AsnAsn: 2.152 ± 0.022	2.303AsnPro: 2.303 ± 0.016	1.615AsnGln: 1.615 ± 0.018	2.117AsnArg: 2.117 ± 0.016	2.764AsnSer: 2.764 ± 0.019	2.585AsnThr: 2.585 ± 0.021	2.653AsnVal: 2.653 ± 0.017	0.481AsnTrp: 0.481 ± 0.007	1.218AsnTyr: 1.218 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	3.952ProAla: 3.952 ± 0.032	1.2ProCys: 1.2 ± 0.017	3.322ProAsp: 3.322 ± 0.027	3.345ProGlu: 3.345 ± 0.027	1.725ProPhe: 1.725 ± 0.013	4.05ProGly: 4.05 ± 0.041	1.329ProHis: 1.329 ± 0.013	1.858ProIle: 1.858 ± 0.018	2.321ProLys: 2.321 ± 0.019	3.757ProLeu: 3.757 ± 0.021	1.036ProMet: 1.036 ± 0.01	2.077ProAsn: 2.077 ± 0.018	4.284ProPro: 4.284 ± 0.045	2.173ProGln: 2.173 ± 0.021	2.745ProArg: 2.745 ± 0.021	4.328ProSer: 4.328 ± 0.03	3.499ProThr: 3.499 ± 0.033	3.643ProVal: 3.643 ± 0.027	0.701ProTrp: 0.701 ± 0.011	2.12ProTyr: 2.12 ± 0.028	0.001ProXaa: 0.001 ± 0.0
Gln	3.192GlnAla: 3.192 ± 0.023	1.135GlnCys: 1.135 ± 0.016	2.518GlnAsp: 2.518 ± 0.019	3.083GlnGlu: 3.083 ± 0.023	1.491GlnPhe: 1.491 ± 0.019	2.745GlnGly: 2.745 ± 0.022	1.349GlnHis: 1.349 ± 0.015	1.662GlnIle: 1.662 ± 0.014	2.356GlnLys: 2.356 ± 0.02	4.026GlnLeu: 4.026 ± 0.029	1.063GlnMet: 1.063 ± 0.011	1.776GlnAsn: 1.776 ± 0.015	2.409GlnPro: 2.409 ± 0.022	2.801GlnGln: 2.801 ± 0.042	2.416GlnArg: 2.416 ± 0.019	2.889GlnSer: 2.889 ± 0.024	2.717GlnThr: 2.717 ± 0.026	2.819GlnVal: 2.819 ± 0.02	0.553GlnTrp: 0.553 ± 0.008	1.297GlnTyr: 1.297 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	3.538ArgAla: 3.538 ± 0.024	1.515ArgCys: 1.515 ± 0.019	3.213ArgAsp: 3.213 ± 0.025	3.607ArgGlu: 3.607 ± 0.025	1.903ArgPhe: 1.903 ± 0.014	3.428ArgGly: 3.428 ± 0.025	1.851ArgHis: 1.851 ± 0.018	2.241ArgIle: 2.241 ± 0.015	3.552ArgLys: 3.552 ± 0.023	5.103ArgLeu: 5.103 ± 0.028	1.269ArgMet: 1.269 ± 0.011	2.234ArgAsn: 2.234 ± 0.017	2.885ArgPro: 2.885 ± 0.019	2.815ArgGln: 2.815 ± 0.024	4.335ArgArg: 4.335 ± 0.031	3.889ArgSer: 3.889 ± 0.027	3.689ArgThr: 3.689 ± 0.029	3.407ArgVal: 3.407 ± 0.023	0.806ArgTrp: 0.806 ± 0.01	1.782ArgTyr: 1.782 ± 0.014	0.001ArgXaa: 0.001 ± 0.0
Ser	5.131SerAla: 5.131 ± 0.029	1.891SerCys: 1.891 ± 0.019	4.365SerAsp: 4.365 ± 0.034	3.964SerGlu: 3.964 ± 0.025	2.63SerPhe: 2.63 ± 0.015	5.353SerGly: 5.353 ± 0.033	1.938SerHis: 1.938 ± 0.018	3.069SerIle: 3.069 ± 0.022	3.397SerLys: 3.397 ± 0.023	6.725SerLeu: 6.725 ± 0.038	1.598SerMet: 1.598 ± 0.013	2.883SerAsn: 2.883 ± 0.02	4.394SerPro: 4.394 ± 0.036	3.159SerGln: 3.159 ± 0.024	4.254SerArg: 4.254 ± 0.031	7.064SerSer: 7.064 ± 0.053	4.895SerThr: 4.895 ± 0.034	4.785SerVal: 4.785 ± 0.026	1.014SerTrp: 1.014 ± 0.011	2.256SerTyr: 2.256 ± 0.023	0.001SerXaa: 0.001 ± 0.0
Thr	4.942ThrAla: 4.942 ± 0.029	2.036ThrCys: 2.036 ± 0.034	3.613ThrAsp: 3.613 ± 0.028	3.752ThrGlu: 3.752 ± 0.023	2.444ThrPhe: 2.444 ± 0.02	5.013ThrGly: 5.013 ± 0.042	1.828ThrHis: 1.828 ± 0.026	2.843ThrIle: 2.843 ± 0.021	2.812ThrLys: 2.812 ± 0.024	5.217ThrLeu: 5.217 ± 0.03	1.414ThrMet: 1.414 ± 0.014	2.384ThrAsn: 2.384 ± 0.018	3.609ThrPro: 3.609 ± 0.028	2.271ThrGln: 2.271 ± 0.026	2.958ThrArg: 2.958 ± 0.019	5.088ThrSer: 5.088 ± 0.027	4.775ThrThr: 4.775 ± 0.108	4.904ThrVal: 4.904 ± 0.033	0.905ThrTrp: 0.905 ± 0.015	1.86ThrTyr: 1.86 ± 0.018	0.001ThrXaa: 0.001 ± 0.0
Val	4.604ValAla: 4.604 ± 0.02	1.798ValCys: 1.798 ± 0.017	3.9ValAsp: 3.9 ± 0.029	4.026ValGlu: 4.026 ± 0.029	2.683ValPhe: 2.683 ± 0.018	4.148ValGly: 4.148 ± 0.027	1.719ValHis: 1.719 ± 0.015	3.112ValIle: 3.112 ± 0.021	3.303ValLys: 3.303 ± 0.022	6.222ValLeu: 6.222 ± 0.027	1.611ValMet: 1.611 ± 0.012	2.514ValAsn: 2.514 ± 0.018	3.555ValPro: 3.555 ± 0.023	2.982ValGln: 2.982 ± 0.022	3.685ValArg: 3.685 ± 0.021	5.08ValSer: 5.08 ± 0.031	4.87ValThr: 4.87 ± 0.041	5.128ValVal: 5.128 ± 0.03	0.902ValTrp: 0.902 ± 0.01	2.155ValTyr: 2.155 ± 0.016	0.001ValXaa: 0.001 ± 0.0
Trp	0.759TrpAla: 0.759 ± 0.009	0.306TrpCys: 0.306 ± 0.006	0.746TrpAsp: 0.746 ± 0.009	0.852TrpGlu: 0.852 ± 0.01	0.519TrpPhe: 0.519 ± 0.008	0.807TrpGly: 0.807 ± 0.011	0.316TrpHis: 0.316 ± 0.007	0.573TrpIle: 0.573 ± 0.008	0.754TrpLys: 0.754 ± 0.015	1.23TrpLeu: 1.23 ± 0.013	0.341TrpMet: 0.341 ± 0.006	0.573TrpAsn: 0.573 ± 0.008	0.541TrpPro: 0.541 ± 0.009	0.535TrpGln: 0.535 ± 0.008	0.864TrpArg: 0.864 ± 0.018	1.058TrpSer: 1.058 ± 0.013	0.875TrpThr: 0.875 ± 0.01	0.834TrpVal: 0.834 ± 0.008	0.245TrpTrp: 0.245 ± 0.006	0.451TrpTyr: 0.451 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.691TyrAla: 1.691 ± 0.015	0.775TyrCys: 0.775 ± 0.011	1.734TyrAsp: 1.734 ± 0.019	1.647TyrGlu: 1.647 ± 0.015	1.207TyrPhe: 1.207 ± 0.012	2.121TyrGly: 2.121 ± 0.02	0.902TyrHis: 0.902 ± 0.011	1.503TyrIle: 1.503 ± 0.014	1.785TyrLys: 1.785 ± 0.019	2.663TyrLeu: 2.663 ± 0.019	0.924TyrMet: 0.924 ± 0.011	1.459TyrAsn: 1.459 ± 0.013	1.402TyrPro: 1.402 ± 0.014	1.304TyrGln: 1.304 ± 0.014	2.15TyrArg: 2.15 ± 0.02	2.407TyrSer: 2.407 ± 0.023	1.929TyrThr: 1.929 ± 0.017	1.971TyrVal: 1.971 ± 0.015	0.482TyrTrp: 0.482 ± 0.009	1.107TyrTyr: 1.107 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.002XaaSer: 0.002 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 28544 proteins (13248417 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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