Haemophilus phage Aaphi23

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q776W9|Q776W9_9CAUD Uncharacterized protein OS=Haemophilus phage Aaphi23 OX=230158 PE=4 SV=1
MM1 pKa = 7.67ANFIKK6 pKa = 10.63LSTINSVIFINMDD19 pKa = 4.35LIEE22 pKa = 4.63GMSRR26 pKa = 11.84DD27 pKa = 3.64DD28 pKa = 4.73SMTSLHH34 pKa = 6.75CSGDD38 pKa = 3.33ANSYY42 pKa = 9.87YY43 pKa = 10.42RR44 pKa = 11.84VTEE47 pKa = 4.0TPEE50 pKa = 4.36EE51 pKa = 3.81ILEE54 pKa = 4.22TMKK57 pKa = 10.9AAEE60 pKa = 4.04

Molecular weight: 6.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q776X1|Q776X1_9CAUD Isoform of Q7Y5U8 Putative lytic protein Rz1 OS=Haemophilus phage Aaphi23 OX=230158 GN=terS PE=4 SV=1
MM1 pKa = 7.29SRR3 pKa = 11.84QIISLKK9 pKa = 9.91KK10 pKa = 10.04RR11 pKa = 11.84GEE13 pKa = 3.47IWHH16 pKa = 6.34YY17 pKa = 11.43SFTSPNGEE25 pKa = 3.62RR26 pKa = 11.84VRR28 pKa = 11.84RR29 pKa = 11.84SARR32 pKa = 11.84TSDD35 pKa = 3.34KK36 pKa = 11.32NQAQQLASKK45 pKa = 9.75EE46 pKa = 4.06YY47 pKa = 8.37NEE49 pKa = 4.29CWRR52 pKa = 11.84VFKK55 pKa = 11.01LGEE58 pKa = 4.02RR59 pKa = 11.84PNYY62 pKa = 8.46SWQEE66 pKa = 3.83AVVQWLDD73 pKa = 3.6EE74 pKa = 4.2KK75 pKa = 10.83PKK77 pKa = 10.56RR78 pKa = 11.84KK79 pKa = 8.93QDD81 pKa = 3.13RR82 pKa = 11.84NMLYY86 pKa = 11.0GLVWLDD92 pKa = 4.25KK93 pKa = 11.09YY94 pKa = 11.45LGDD97 pKa = 4.48KK98 pKa = 10.11KK99 pKa = 11.01LNEE102 pKa = 4.11IDD104 pKa = 3.12RR105 pKa = 11.84TLIKK109 pKa = 10.29FIQYY113 pKa = 8.83EE114 pKa = 4.13KK115 pKa = 10.78AKK117 pKa = 9.79EE118 pKa = 4.04GVKK121 pKa = 10.47ARR123 pKa = 11.84TINAVLQQIRR133 pKa = 11.84VILRR137 pKa = 11.84AAVEE141 pKa = 4.09WDD143 pKa = 3.28WLDD146 pKa = 3.26KK147 pKa = 11.23CPAIKK152 pKa = 10.16FLPEE156 pKa = 3.42PKK158 pKa = 9.93RR159 pKa = 11.84RR160 pKa = 11.84VRR162 pKa = 11.84WLTQHH167 pKa = 6.06EE168 pKa = 4.71EE169 pKa = 3.42IRR171 pKa = 11.84LIEE174 pKa = 4.33EE175 pKa = 4.41LPEE178 pKa = 3.93HH179 pKa = 6.92LKK181 pKa = 10.82PIVQFAILTGLRR193 pKa = 11.84MSNITQLKK201 pKa = 8.34WSQIDD206 pKa = 3.62LSKK209 pKa = 10.64RR210 pKa = 11.84QAWINSEE217 pKa = 3.89QSKK220 pKa = 8.83TGNSIGVPLNDD231 pKa = 3.74KK232 pKa = 10.4AIEE235 pKa = 4.33VIVSQFGKK243 pKa = 9.97HH244 pKa = 5.27KK245 pKa = 10.78EE246 pKa = 3.93NVFTYY251 pKa = 9.48KK252 pKa = 10.66GKK254 pKa = 9.54PVRR257 pKa = 11.84IANTKK262 pKa = 9.76AFRR265 pKa = 11.84AALQRR270 pKa = 11.84AGIKK274 pKa = 10.17DD275 pKa = 3.71FRR277 pKa = 11.84FHH279 pKa = 7.69DD280 pKa = 4.87LRR282 pKa = 11.84HH283 pKa = 5.01TWATRR288 pKa = 11.84HH289 pKa = 5.7IMSGTPLYY297 pKa = 10.62VLQEE301 pKa = 3.87LGGWSKK307 pKa = 11.67SDD309 pKa = 3.32TVRR312 pKa = 11.84KK313 pKa = 8.85YY314 pKa = 9.46AHH316 pKa = 6.89LSVEE320 pKa = 4.15HH321 pKa = 6.17LQNHH325 pKa = 6.43ANNVQLFATKK335 pKa = 10.39LAQTQRR341 pKa = 11.84GKK343 pKa = 10.57NLINNN348 pKa = 4.41

Molecular weight: 40.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

1

65

13554

27

800

208.5

23.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.175 ± 0.392

0.907 ± 0.112

5.799 ± 0.214

6.625 ± 0.322

4.25 ± 0.197

6.367 ± 0.269

1.601 ± 0.127

6.817 ± 0.201

7.769 ± 0.343

9.355 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.206 ± 0.143

5.26 ± 0.194

2.973 ± 0.149

4.67 ± 0.201

4.663 ± 0.298

6.22 ± 0.215

5.829 ± 0.245

5.969 ± 0.181

1.409 ± 0.118

3.136 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski