Flavobacterium aquatile LMG 4008 = ATCC 11947

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; Flavobacterium aquatile

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2995 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A095TYF8|A0A095TYF8_9FLAO Mechanosensitive ion channel protein MscS OS=Flavobacterium aquatile LMG 4008 = ATCC 11947 OX=1453498 GN=LG45_14555 PE=4 SV=1
MM1 pKa = 7.11KK2 pKa = 10.27TNFKK6 pKa = 10.98NIGLVVLATLAFVSCSDD23 pKa = 3.93DD24 pKa = 3.81NPVEE28 pKa = 4.64GNQAPSAAEE37 pKa = 3.75FDD39 pKa = 4.07NVRR42 pKa = 11.84QTALDD47 pKa = 3.64NLTQNFTLVAEE58 pKa = 4.95DD59 pKa = 4.07GVTTFTSDD67 pKa = 2.86KK68 pKa = 10.67GVQFSINGNCLLKK81 pKa = 10.88NGNPVTGSVAIEE93 pKa = 3.88YY94 pKa = 10.78VEE96 pKa = 5.6LFDD99 pKa = 5.3AGNMLVTDD107 pKa = 4.28KK108 pKa = 9.0TTMGRR113 pKa = 11.84MPNGDD118 pKa = 3.23MTLLVSGGEE127 pKa = 4.17FYY129 pKa = 10.87INASQGGVDD138 pKa = 5.7LDD140 pKa = 3.83ITCPMQLLIPSTLTGGADD158 pKa = 2.96TGMTLWDD165 pKa = 3.54GTIDD169 pKa = 3.9EE170 pKa = 5.38DD171 pKa = 6.24GNLEE175 pKa = 4.12WDD177 pKa = 3.78EE178 pKa = 4.38QEE180 pKa = 4.66QNPAGQGGVFVEE192 pKa = 4.76GTGVNAPYY200 pKa = 10.65YY201 pKa = 11.01AFFDD205 pKa = 3.79SFGWTNVDD213 pKa = 3.64RR214 pKa = 11.84FYY216 pKa = 11.3SDD218 pKa = 3.14PRR220 pKa = 11.84PRR222 pKa = 11.84TMILAEE228 pKa = 4.55APNGYY233 pKa = 9.91DD234 pKa = 3.44FEE236 pKa = 5.39NSAVYY241 pKa = 9.76LHH243 pKa = 6.73YY244 pKa = 10.96DD245 pKa = 3.86GEE247 pKa = 4.74ASTLAKK253 pKa = 10.39LDD255 pKa = 3.76TYY257 pKa = 11.83NDD259 pKa = 3.28ATNQFSEE266 pKa = 5.04HH267 pKa = 6.39YY268 pKa = 8.61GQIPIGLEE276 pKa = 3.66CHH278 pKa = 6.32VIFVTEE284 pKa = 4.47EE285 pKa = 3.76DD286 pKa = 4.11GQWRR290 pKa = 11.84YY291 pKa = 10.08AIKK294 pKa = 10.47GVTIAADD301 pKa = 3.48DD302 pKa = 4.48VYY304 pKa = 11.16TFTLSEE310 pKa = 4.34TTVGSQAQLVAAINALPP327 pKa = 3.74

Molecular weight: 35.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A095UW20|A0A095UW20_9FLAO Uncharacterized protein OS=Flavobacterium aquatile LMG 4008 = ATCC 11947 OX=1453498 GN=LG45_15090 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.91HH17 pKa = 4.39GFMDD21 pKa = 4.5RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2995

0

2995

982436

46

2404

328.0

37.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.105 ± 0.047

0.779 ± 0.016

5.353 ± 0.031

6.469 ± 0.051

5.553 ± 0.039

6.056 ± 0.043

1.593 ± 0.021

8.373 ± 0.048

8.186 ± 0.065

9.077 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.19 ± 0.023

6.676 ± 0.056

3.27 ± 0.027

3.364 ± 0.025

3.028 ± 0.031

6.702 ± 0.042

5.969 ± 0.069

6.241 ± 0.038

0.972 ± 0.015

4.044 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski