Ruminiclostridium hungatei (Clostridium hungatei)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminiclostridium

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4233 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V4SD50|A0A1V4SD50_RUMHU Uncharacterized protein OS=Ruminiclostridium hungatei OX=48256 GN=CLHUN_42900 PE=4 SV=1
MM1 pKa = 7.73INTNLKK7 pKa = 8.65ATAIFDD13 pKa = 3.69NGGGLTLQLGDD24 pKa = 4.27NYY26 pKa = 11.02GHH28 pKa = 6.71YY29 pKa = 10.0YY30 pKa = 8.31PHH32 pKa = 6.9NMQQAAEE39 pKa = 4.95DD40 pKa = 4.02YY41 pKa = 10.27AQYY44 pKa = 11.05LADD47 pKa = 5.27QDD49 pKa = 4.21TSWWEE54 pKa = 3.92GNEE57 pKa = 3.62DD58 pKa = 3.78DD59 pKa = 5.5ARR61 pKa = 11.84EE62 pKa = 4.13LEE64 pKa = 4.47PEE66 pKa = 4.38LEE68 pKa = 4.24QIRR71 pKa = 11.84NGGYY75 pKa = 9.75KK76 pKa = 9.97VYY78 pKa = 10.23NASDD82 pKa = 3.37IAGLLPMIDD91 pKa = 3.27TQQFKK96 pKa = 10.9EE97 pKa = 4.18DD98 pKa = 3.7GYY100 pKa = 9.36ITGWYY105 pKa = 9.66NVDD108 pKa = 3.54EE109 pKa = 4.66FVTALSALTNVSII122 pKa = 5.07

Molecular weight: 13.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V4SM13|A0A1V4SM13_RUMHU Methyltransf_21 domain-containing protein OS=Ruminiclostridium hungatei OX=48256 GN=CLHUN_14540 PE=4 SV=1
MM1 pKa = 7.97KK2 pKa = 10.3IITRR6 pKa = 11.84NNAQFFLLLNAIFWGSSYY24 pKa = 10.27IWSKK28 pKa = 10.47MLLNFLPAFSILFICAALGLAATFLLFRR56 pKa = 11.84RR57 pKa = 11.84QLGNFSRR64 pKa = 11.84KK65 pKa = 9.23IILISTLISFVSVISNTFCMLALKK89 pKa = 8.93RR90 pKa = 11.84TSGSNTAFIVQLSIVITPVIMAILEE115 pKa = 4.14KK116 pKa = 10.36KK117 pKa = 10.03RR118 pKa = 11.84PSLRR122 pKa = 11.84TVLLALSALLGIYY135 pKa = 10.52LITCAGKK142 pKa = 10.63GFNINLGDD150 pKa = 4.61IFALCNAVFFSLFIALQNKK169 pKa = 8.27FSQVVNPVQFTFIQHH184 pKa = 5.88LTNLGAFFVLSLIFEE199 pKa = 4.62SGGIVYY205 pKa = 10.63SNMLSPVFVLLICLNAMVTIFTALFQSSAIKK236 pKa = 10.1YY237 pKa = 7.28VRR239 pKa = 11.84PEE241 pKa = 4.01SATVLYY247 pKa = 10.63AFEE250 pKa = 4.65PVTTAILGATLLGEE264 pKa = 4.44HH265 pKa = 6.88FSGIASVAGCILIFLTVIVSSVRR288 pKa = 11.84LSRR291 pKa = 11.84FSSRR295 pKa = 11.84RR296 pKa = 11.84LRR298 pKa = 11.84LNTSSNIKK306 pKa = 10.47GLL308 pKa = 3.71

Molecular weight: 33.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4233

0

4233

1387579

29

3718

327.8

36.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.256 ± 0.043

1.243 ± 0.014

5.332 ± 0.031

6.776 ± 0.043

4.316 ± 0.028

7.244 ± 0.035

1.488 ± 0.017

8.133 ± 0.034

7.104 ± 0.035

9.209 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.018

5.126 ± 0.035

3.325 ± 0.022

2.977 ± 0.02

4.235 ± 0.03

6.604 ± 0.035

5.317 ± 0.048

6.776 ± 0.031

0.899 ± 0.013

4.004 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski