Lachnospiraceae bacterium XBB1006

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2560 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I5YUI5|A0A1I5YUI5_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium XBB1006 OX=1520827 GN=SAMN02910358_02357 PE=4 SV=1
MM1 pKa = 7.36NEE3 pKa = 4.1LYY5 pKa = 11.03EE6 pKa = 4.35MIEE9 pKa = 3.91QKK11 pKa = 10.12IQSSGYY17 pKa = 9.13RR18 pKa = 11.84RR19 pKa = 11.84PLSGRR24 pKa = 11.84DD25 pKa = 3.59VYY27 pKa = 11.77NDD29 pKa = 2.79ICDD32 pKa = 3.79QIDD35 pKa = 3.44GKK37 pKa = 11.41EE38 pKa = 3.98NGDD41 pKa = 3.7YY42 pKa = 11.35LLLSKK47 pKa = 11.0FEE49 pKa = 4.16EE50 pKa = 4.64DD51 pKa = 3.55VTFEE55 pKa = 3.79YY56 pKa = 10.39HH57 pKa = 4.8ITIMDD62 pKa = 3.55EE63 pKa = 4.04EE64 pKa = 4.64FNLGVLTITAPEE76 pKa = 4.13GTYY79 pKa = 10.79SVDD82 pKa = 4.05FDD84 pKa = 4.72AEE86 pKa = 4.06

Molecular weight: 9.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I5X126|A0A1I5X126_9FIRM Putative GTP pyrophosphokinase OS=Lachnospiraceae bacterium XBB1006 OX=1520827 GN=SAMN02910358_01295 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.61VLQSRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.85KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2560

0

2560

814644

39

2207

318.2

35.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.451 ± 0.045

1.504 ± 0.02

5.486 ± 0.044

8.092 ± 0.062

3.978 ± 0.03

6.918 ± 0.05

1.785 ± 0.023

6.916 ± 0.044

7.483 ± 0.056

8.7 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.245 ± 0.029

4.314 ± 0.032

3.096 ± 0.027

3.209 ± 0.031

4.471 ± 0.04

5.497 ± 0.035

5.535 ± 0.048

7.337 ± 0.05

0.822 ± 0.016

4.159 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski