gamma proteobacterium HTCC2207

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Porticoccaceae; unclassified Porticoccaceae; SAR92 clade

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2388 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1YR40|Q1YR40_9GAMM Gln-synt_C domain-containing protein OS=gamma proteobacterium HTCC2207 OX=314287 GN=GB2207_03989 PE=3 SV=1
MM1 pKa = 7.1THH3 pKa = 5.13TKK5 pKa = 9.45IRR7 pKa = 11.84KK8 pKa = 6.22TLISAAVAAATLSAGQLSAAQIEE31 pKa = 4.71EE32 pKa = 4.57IIVTTQKK39 pKa = 10.43RR40 pKa = 11.84QQSLQDD46 pKa = 3.43VPISVSAFSGSFIEE60 pKa = 4.85KK61 pKa = 10.33AQISDD66 pKa = 3.57AKK68 pKa = 10.25QVALLTPGVSGDD80 pKa = 3.72TDD82 pKa = 4.38DD83 pKa = 6.04SFLDD87 pKa = 3.55SMNVRR92 pKa = 11.84GISTNDD98 pKa = 3.16FGVGAEE104 pKa = 4.22PSIGLYY110 pKa = 9.88QDD112 pKa = 4.72GIYY115 pKa = 10.4LGRR118 pKa = 11.84TGGAVSSFFDD128 pKa = 3.45VEE130 pKa = 4.09MVEE133 pKa = 4.64VVKK136 pKa = 10.93GPQGTLFGRR145 pKa = 11.84NASSGAISITTAKK158 pKa = 8.57PTGEE162 pKa = 4.2AGGSIDD168 pKa = 4.98IGFGQDD174 pKa = 2.95GYY176 pKa = 11.84GEE178 pKa = 4.22VTAVINTPINDD189 pKa = 3.61QFSGRR194 pKa = 11.84LAVYY198 pKa = 9.25HH199 pKa = 5.2QQQDD203 pKa = 3.22GWVTNINDD211 pKa = 3.87GQQTGGVEE219 pKa = 3.99NTAARR224 pKa = 11.84YY225 pKa = 7.44TLAFEE230 pKa = 4.85NDD232 pKa = 4.84DD233 pKa = 3.55ITSTLMLEE241 pKa = 4.38YY242 pKa = 9.91EE243 pKa = 4.63DD244 pKa = 4.72RR245 pKa = 11.84QGPPTIYY252 pKa = 10.16QAFDD256 pKa = 3.7PDD258 pKa = 3.67EE259 pKa = 4.57TGLPFYY265 pKa = 10.56STDD268 pKa = 3.28AAEE271 pKa = 4.68DD272 pKa = 3.56QFTSDD277 pKa = 3.68VSSSDD282 pKa = 4.26LIDD285 pKa = 3.94EE286 pKa = 4.61GEE288 pKa = 4.27VWGVTLNVEE297 pKa = 3.9VDD299 pKa = 3.63LGNDD303 pKa = 3.44YY304 pKa = 9.16TLSSTTGLRR313 pKa = 11.84GHH315 pKa = 6.67NYY317 pKa = 10.31YY318 pKa = 10.79YY319 pKa = 11.06LEE321 pKa = 4.9DD322 pKa = 4.17FDD324 pKa = 5.55GGSSFLSNYY333 pKa = 8.92NQIQEE338 pKa = 3.74QDD340 pKa = 3.61YY341 pKa = 9.79FSQEE345 pKa = 3.34FRR347 pKa = 11.84INKK350 pKa = 7.0EE351 pKa = 3.79TDD353 pKa = 3.31SVSWFVGASWYY364 pKa = 10.5KK365 pKa = 10.57EE366 pKa = 3.69EE367 pKa = 4.72LKK369 pKa = 11.08VRR371 pKa = 11.84FNQTLDD377 pKa = 3.34EE378 pKa = 4.47DD379 pKa = 4.76TFCAAYY385 pKa = 8.19GTYY388 pKa = 10.12YY389 pKa = 10.44EE390 pKa = 4.77YY391 pKa = 11.32DD392 pKa = 5.31DD393 pKa = 4.12ITDD396 pKa = 3.74CATLYY401 pKa = 10.46EE402 pKa = 4.51YY403 pKa = 11.39YY404 pKa = 10.13EE405 pKa = 4.18YY406 pKa = 11.26DD407 pKa = 4.41PIVGIGTRR415 pKa = 11.84NDD417 pKa = 3.63SVDD420 pKa = 2.79VDD422 pKa = 3.84AEE424 pKa = 4.09YY425 pKa = 11.04DD426 pKa = 3.32GWGLYY431 pKa = 10.46GDD433 pKa = 3.8ATFHH437 pKa = 6.53VNEE440 pKa = 4.94DD441 pKa = 3.01LDD443 pKa = 4.7FIVGARR449 pKa = 11.84YY450 pKa = 8.09TEE452 pKa = 4.3DD453 pKa = 3.31NRR455 pKa = 11.84DD456 pKa = 3.1FAQNFGGEE464 pKa = 4.2EE465 pKa = 4.25RR466 pKa = 11.84NATWYY471 pKa = 7.53TFPFYY476 pKa = 10.64TSDD479 pKa = 3.54FVQGNDD485 pKa = 2.37KK486 pKa = 8.41WTNTSVRR493 pKa = 11.84AAVNYY498 pKa = 9.58QLSDD502 pKa = 3.59EE503 pKa = 4.37VSTYY507 pKa = 9.31ATYY510 pKa = 9.3STGYY514 pKa = 9.44KK515 pKa = 10.25AGGFNTMEE523 pKa = 4.68LSFADD528 pKa = 5.37DD529 pKa = 3.39IAVDD533 pKa = 4.15DD534 pKa = 5.23LDD536 pKa = 6.47GEE538 pKa = 4.58DD539 pKa = 5.56ALDD542 pKa = 4.29FDD544 pKa = 5.22PSLASFDD551 pKa = 4.0KK552 pKa = 11.49EE553 pKa = 3.86EE554 pKa = 4.08VTNLEE559 pKa = 4.8LGLKK563 pKa = 10.04GQFLDD568 pKa = 5.0GQMQLNAALYY578 pKa = 9.53SYY580 pKa = 10.62QFDD583 pKa = 4.03GMQSGYY589 pKa = 10.59YY590 pKa = 9.46VDD592 pKa = 4.26GRR594 pKa = 11.84YY595 pKa = 7.16TVANVGDD602 pKa = 4.0AEE604 pKa = 4.49GSGLEE609 pKa = 3.96LDD611 pKa = 3.96MRR613 pKa = 11.84YY614 pKa = 10.11LPTDD618 pKa = 3.01NVDD621 pKa = 3.21IYY623 pKa = 11.4LGLAWADD630 pKa = 3.75SEE632 pKa = 4.4LTKK635 pKa = 10.78PNSSLSADD643 pKa = 3.69FCEE646 pKa = 4.97DD647 pKa = 3.45DD648 pKa = 4.03CTGAMLPGTVDD659 pKa = 4.08FSAALVATYY668 pKa = 10.29SRR670 pKa = 11.84PIEE673 pKa = 4.57GGDD676 pKa = 4.68LNLTWEE682 pKa = 4.51TFHH685 pKa = 7.33QGASPGFGDD694 pKa = 5.1FSRR697 pKa = 11.84EE698 pKa = 3.76PIMLDD703 pKa = 2.86EE704 pKa = 4.51FTVSNLRR711 pKa = 11.84VGYY714 pKa = 10.18DD715 pKa = 3.38SSDD718 pKa = 3.16NWSMTVWVNNVFDD731 pKa = 3.47TFYY734 pKa = 10.77YY735 pKa = 10.36KK736 pKa = 10.68GVAPADD742 pKa = 4.51GIIAPHH748 pKa = 5.97YY749 pKa = 10.28FGFSEE754 pKa = 4.29PRR756 pKa = 11.84RR757 pKa = 11.84MGLDD761 pKa = 3.5LAYY764 pKa = 9.92KK765 pKa = 9.96FF766 pKa = 4.42

Molecular weight: 84.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1YV64|Q1YV64_9GAMM Flagellar motor switch protein FliG OS=gamma proteobacterium HTCC2207 OX=314287 GN=GB2207_08546 PE=3 SV=1
MM1 pKa = 7.86PKK3 pKa = 7.87THH5 pKa = 7.07KK6 pKa = 10.45AFHH9 pKa = 6.06HH10 pKa = 6.34TPHH13 pKa = 6.36KK14 pKa = 9.15TGDD17 pKa = 3.72YY18 pKa = 10.38AALAHH23 pKa = 6.43FSDD26 pKa = 4.03RR27 pKa = 11.84ATWRR31 pKa = 11.84QSTVARR37 pKa = 11.84THH39 pKa = 5.95SVPAFWRR46 pKa = 11.84DD47 pKa = 3.11WLLHH51 pKa = 6.29PGSLTQRR58 pKa = 11.84LLDD61 pKa = 4.16ASDD64 pKa = 3.72GQFKK68 pKa = 11.05VGVLSQSLLRR78 pKa = 11.84PSLSEE83 pKa = 3.82RR84 pKa = 11.84RR85 pKa = 11.84ALSLPDD91 pKa = 3.35HH92 pKa = 6.77RR93 pKa = 11.84LALVRR98 pKa = 11.84EE99 pKa = 4.43VILIGRR105 pKa = 11.84GVPWVFARR113 pKa = 11.84SVIPLQTLTGRR124 pKa = 11.84LRR126 pKa = 11.84KK127 pKa = 9.41LRR129 pKa = 11.84RR130 pKa = 11.84LDD132 pKa = 3.6SRR134 pKa = 11.84PLGALLFSDD143 pKa = 3.44STMTRR148 pKa = 11.84EE149 pKa = 3.95PLEE152 pKa = 4.16WACIEE157 pKa = 4.62PNGQPLAAEE166 pKa = 4.82LEE168 pKa = 4.43AMDD171 pKa = 3.9KK172 pKa = 10.48PVWGRR177 pKa = 11.84RR178 pKa = 11.84SVFKK182 pKa = 10.86LSAKK186 pKa = 9.98PMLVCEE192 pKa = 4.24VFLPSFSLQDD202 pKa = 3.28VANNLTRR209 pKa = 11.84SS210 pKa = 3.5

Molecular weight: 23.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2388

0

2388

778587

21

2403

326.0

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.686 ± 0.053

1.015 ± 0.019

5.938 ± 0.037

6.067 ± 0.042

3.903 ± 0.032

7.623 ± 0.051

1.982 ± 0.022

6.157 ± 0.036

4.298 ± 0.036

10.258 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.612 ± 0.026

3.835 ± 0.031

4.15 ± 0.028

4.339 ± 0.037

5.036 ± 0.04

6.851 ± 0.047

5.197 ± 0.035

7.055 ± 0.043

1.235 ± 0.018

2.762 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski