Mycobacterium phage Severus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4JQ77|R4JQ77_9CAUD DNA primase OS=Mycobacterium phage Severus OX=1327776 GN=54 PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84NPYY5 pKa = 10.06GDD7 pKa = 3.78SPEE10 pKa = 4.2YY11 pKa = 10.92DD12 pKa = 2.83SDD14 pKa = 3.83DD15 pKa = 4.02PYY17 pKa = 11.54RR18 pKa = 11.84PGGPLADD25 pKa = 4.31AQLAWEE31 pKa = 4.92AGQPGYY37 pKa = 8.48TAAQFRR43 pKa = 11.84QDD45 pKa = 3.13VGII48 pKa = 4.33

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4JMP3|R4JMP3_9CAUD Uncharacterized protein OS=Mycobacterium phage Severus OX=1327776 GN=48 PE=4 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84KK3 pKa = 9.13KK4 pKa = 9.94HH5 pKa = 6.0LKK7 pKa = 9.48AALRR11 pKa = 11.84AANASIEE18 pKa = 4.1EE19 pKa = 4.39LGQSNTQLWRR29 pKa = 11.84EE30 pKa = 4.02RR31 pKa = 11.84EE32 pKa = 4.09DD33 pKa = 4.45LRR35 pKa = 11.84QEE37 pKa = 3.96NQALKK42 pKa = 10.62LKK44 pKa = 10.46LGSASDD50 pKa = 3.81LFGEE54 pKa = 4.49AFVKK58 pKa = 10.55GGQLPPPKK66 pKa = 10.51GPNRR70 pKa = 11.84PNAPKK75 pKa = 10.56LSRR78 pKa = 11.84RR79 pKa = 11.84DD80 pKa = 3.4AEE82 pKa = 4.38HH83 pKa = 6.72IRR85 pKa = 11.84DD86 pKa = 3.75LVRR89 pKa = 11.84AGNSRR94 pKa = 11.84RR95 pKa = 11.84EE96 pKa = 3.66VARR99 pKa = 11.84AYY101 pKa = 10.11DD102 pKa = 3.74INPATVSRR110 pKa = 11.84IVRR113 pKa = 11.84GTYY116 pKa = 9.8YY117 pKa = 10.69RR118 pKa = 3.92

Molecular weight: 13.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

15433

24

851

192.9

21.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.296 ± 0.361

0.914 ± 0.119

6.434 ± 0.189

6.629 ± 0.305

3.078 ± 0.162

8.041 ± 0.337

2.054 ± 0.19

4.594 ± 0.15

4.205 ± 0.195

8.436 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.12

3.363 ± 0.201

5.832 ± 0.282

3.577 ± 0.151

6.998 ± 0.371

5.242 ± 0.298

5.935 ± 0.218

7.173 ± 0.239

2.099 ± 0.125

2.773 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski