Mesorhizobium sp. Root172

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium; unclassified Mesorhizobium

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6374 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8LEA8|A0A0Q8LEA8_9RHIZ UDP-glucose 4-epimerase OS=Mesorhizobium sp. Root172 OX=1736481 GN=ASE05_28810 PE=4 SV=1
MM1 pKa = 7.08YY2 pKa = 10.21RR3 pKa = 11.84LLVASAGLLAALSMPAFAADD23 pKa = 3.65PTVDD27 pKa = 3.51VPMTAPGFDD36 pKa = 2.9WTGYY40 pKa = 9.38YY41 pKa = 10.52AGLQAGYY48 pKa = 10.27GWGQSDD54 pKa = 3.08ISGTEE59 pKa = 3.85GEE61 pKa = 4.66PFSVSPDD68 pKa = 2.9IDD70 pKa = 3.42GGFVGGHH77 pKa = 5.27VAGLWQFDD85 pKa = 3.57QAVIGAEE92 pKa = 3.63ADD94 pKa = 3.69LNYY97 pKa = 10.69SAVDD101 pKa = 3.54GATEE105 pKa = 4.15AGAGNNFGTDD115 pKa = 3.17IKK117 pKa = 10.86WFGSVNAKK125 pKa = 10.11AGYY128 pKa = 10.97AMDD131 pKa = 3.81RR132 pKa = 11.84VLVYY136 pKa = 10.36GIGGIAFAGVEE147 pKa = 4.28TSQASGSAFSQTRR160 pKa = 11.84TNVGWTVGAGVDD172 pKa = 3.58YY173 pKa = 11.25ALTDD177 pKa = 3.51KK178 pKa = 10.86FVVGAQYY185 pKa = 10.68RR186 pKa = 11.84YY187 pKa = 8.61YY188 pKa = 11.07DD189 pKa = 4.17FGSEE193 pKa = 4.44HH194 pKa = 7.08YY195 pKa = 10.34DD196 pKa = 3.32GSADD200 pKa = 4.24FVGRR204 pKa = 11.84DD205 pKa = 3.23QDD207 pKa = 3.8TKK209 pKa = 11.49LNTVGINLSYY219 pKa = 11.13KK220 pKa = 10.18FF221 pKa = 3.9

Molecular weight: 23.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8KHT7|A0A0Q8KHT7_9RHIZ 2-deoxy-D-ribose 5-phosphate aldolase OS=Mesorhizobium sp. Root172 OX=1736481 GN=ASE05_17670 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.11GGRR28 pKa = 11.84GVVAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6374

0

6374

1980124

29

10058

310.7

33.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.467 ± 0.04

0.8 ± 0.01

5.732 ± 0.024

5.393 ± 0.034

3.886 ± 0.021

8.67 ± 0.035

2.014 ± 0.015

5.462 ± 0.024

3.696 ± 0.024

9.923 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.519 ± 0.014

2.703 ± 0.022

5.027 ± 0.027

3.0 ± 0.015

6.748 ± 0.039

5.581 ± 0.028

5.314 ± 0.032

7.477 ± 0.025

1.347 ± 0.013

2.239 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski