Rotavirus G pigeon/HK18

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus G

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3R2P5|U3R2P5_9REOV VP3 OS=Rotavirus G pigeon/HK18 OX=1399970 PE=4 SV=1
MM1 pKa = 7.92AEE3 pKa = 3.97LLKK6 pKa = 11.13VCFAEE11 pKa = 4.31MMKK14 pKa = 10.41SGNCSFEE21 pKa = 4.4SFMEE25 pKa = 4.12KK26 pKa = 9.52MDD28 pKa = 4.31DD29 pKa = 3.63AGINVDD35 pKa = 3.42AWKK38 pKa = 9.99QAYY41 pKa = 9.64DD42 pKa = 3.66GNKK45 pKa = 9.57LPKK48 pKa = 10.34RR49 pKa = 11.84MTKK52 pKa = 8.6STLAIQNSNLEE63 pKa = 4.16KK64 pKa = 10.65EE65 pKa = 4.48IVTLRR70 pKa = 11.84TKK72 pKa = 7.46EE73 pKa = 3.79HH74 pKa = 6.43RR75 pKa = 11.84KK76 pKa = 10.48GYY78 pKa = 10.38DD79 pKa = 2.91KK80 pKa = 11.36NKK82 pKa = 8.78RR83 pKa = 11.84TLANFDD89 pKa = 3.56VGKK92 pKa = 10.94KK93 pKa = 10.03NGNTVLIPKK102 pKa = 8.57TRR104 pKa = 11.84LAEE107 pKa = 4.73IILLNSSHH115 pKa = 6.9DD116 pKa = 4.39LKK118 pKa = 11.33LSAPPSDD125 pKa = 3.76YY126 pKa = 10.65IEE128 pKa = 4.19YY129 pKa = 11.0LEE131 pKa = 5.74DD132 pKa = 3.56EE133 pKa = 4.13NSKK136 pKa = 10.95LKK138 pKa = 10.82EE139 pKa = 3.87EE140 pKa = 4.72NFNLMQNNHH149 pKa = 5.64YY150 pKa = 10.59LYY152 pKa = 9.78NQYY155 pKa = 10.55HH156 pKa = 6.59ALHH159 pKa = 5.93VLSYY163 pKa = 11.16GLQEE167 pKa = 3.95TNNWQKK173 pKa = 10.93EE174 pKa = 4.52VIQRR178 pKa = 11.84MQEE181 pKa = 3.9NEE183 pKa = 3.94FEE185 pKa = 4.24MSSEE189 pKa = 3.86INDD192 pKa = 3.69LKK194 pKa = 11.26SVVRR198 pKa = 11.84TLSRR202 pKa = 11.84RR203 pKa = 11.84LNYY206 pKa = 10.54DD207 pKa = 2.59VDD209 pKa = 4.3FDD211 pKa = 4.24EE212 pKa = 6.39EE213 pKa = 4.57SDD215 pKa = 3.3QGYY218 pKa = 10.1SSDD221 pKa = 4.62VIIEE225 pKa = 3.98SDD227 pKa = 3.44QEE229 pKa = 4.35DD230 pKa = 4.45NINVDD235 pKa = 3.47EE236 pKa = 4.58NDD238 pKa = 3.64NNEE241 pKa = 4.39HH242 pKa = 7.32DD243 pKa = 3.97SSADD247 pKa = 3.31EE248 pKa = 4.17NEE250 pKa = 4.31IIEE253 pKa = 5.06DD254 pKa = 4.07LDD256 pKa = 4.29DD257 pKa = 5.24LLEE260 pKa = 4.28QYY262 pKa = 7.21EE263 pKa = 4.19QRR265 pKa = 11.84QRR267 pKa = 11.84MEE269 pKa = 3.84MARR272 pKa = 11.84NALVEE277 pKa = 4.12LQIQDD282 pKa = 4.7DD283 pKa = 4.38EE284 pKa = 4.91NSDD287 pKa = 3.8DD288 pKa = 3.61EE289 pKa = 5.61DD290 pKa = 4.99YY291 pKa = 11.63NFLYY295 pKa = 10.54FGEE298 pKa = 4.5RR299 pKa = 11.84EE300 pKa = 4.12HH301 pKa = 7.34RR302 pKa = 3.8

Molecular weight: 35.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U3QY89|U3QY89_9REOV RNA-directed RNA polymerase OS=Rotavirus G pigeon/HK18 OX=1399970 PE=3 SV=1
MM1 pKa = 7.69GSHH4 pKa = 5.48QSSYY8 pKa = 9.5QVNNQNTIISNSKK21 pKa = 10.09NLDD24 pKa = 3.72FKK26 pKa = 11.06PSTSSALSQVNLFFVIGAAVGVFLLTALIVSVILNIYY63 pKa = 8.84LCRR66 pKa = 11.84RR67 pKa = 11.84VKK69 pKa = 10.56NGKK72 pKa = 9.34KK73 pKa = 10.31YY74 pKa = 10.42NGRR77 pKa = 11.84TNFQSGTTSRR87 pKa = 11.84HH88 pKa = 3.6NTKK91 pKa = 9.55MDD93 pKa = 3.39EE94 pKa = 4.25HH95 pKa = 6.54EE96 pKa = 4.75HH97 pKa = 5.84LQSSANVV104 pKa = 3.3

Molecular weight: 11.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

5701

104

1160

475.1

54.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.683 ± 0.457

1.07 ± 0.277

6.683 ± 0.494

6.841 ± 0.576

3.894 ± 0.36

3.684 ± 0.231

1.561 ± 0.203

8.314 ± 0.318

7.823 ± 0.537

7.806 ± 0.556

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.245 ± 0.234

6.806 ± 0.574

3.052 ± 0.354

3.982 ± 0.254

5.087 ± 0.311

6.999 ± 0.375

6.016 ± 0.39

6.209 ± 0.28

0.772 ± 0.13

4.455 ± 0.518
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski