Strawberry chlorotic fleck-associated virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Closterovirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0GK54|Q0GK54_9CLOS RdRp (Fragment) OS=Strawberry chlorotic fleck-associated virus OX=399314 PE=4 SV=1
MM1 pKa = 7.14STLLSLQKK9 pKa = 10.48VNRR12 pKa = 11.84FVCVVVPDD20 pKa = 3.52SWTGDD25 pKa = 3.23VFGFNVNKK33 pKa = 10.41SQANKK38 pKa = 10.0FSYY41 pKa = 10.98GDD43 pKa = 3.4YY44 pKa = 10.59AYY46 pKa = 9.99ISMQEE51 pKa = 4.32DD52 pKa = 3.69LSKK55 pKa = 11.15SHH57 pKa = 6.9VILFAFEE64 pKa = 4.19EE65 pKa = 4.49DD66 pKa = 3.21NMYY69 pKa = 10.04HH70 pKa = 6.5IKK72 pKa = 10.49YY73 pKa = 8.85IQTISSSISVSSTDD87 pKa = 3.34EE88 pKa = 3.92VVKK91 pKa = 10.99DD92 pKa = 3.77FTVEE96 pKa = 3.77DD97 pKa = 4.08LSAVKK102 pKa = 8.83FTLRR106 pKa = 11.84VLRR109 pKa = 11.84DD110 pKa = 3.45EE111 pKa = 5.63SILSCEE117 pKa = 4.01VHH119 pKa = 5.75EE120 pKa = 4.49MRR122 pKa = 11.84VKK124 pKa = 10.87VKK126 pKa = 10.28VGYY129 pKa = 10.42SSFIKK134 pKa = 10.44FGTISYY140 pKa = 8.43VPPGEE145 pKa = 4.03GHH147 pKa = 6.73INTVKK152 pKa = 10.55PSFQFVFDD160 pKa = 3.92TLRR163 pKa = 11.84SDD165 pKa = 4.07LSSEE169 pKa = 4.31TVIVEE174 pKa = 4.3SGCPTATACSLTPAKK189 pKa = 10.79SPFSQGALFEE199 pKa = 4.21EE200 pKa = 5.25LEE202 pKa = 4.25ASEE205 pKa = 4.75SYY207 pKa = 10.36RR208 pKa = 11.84DD209 pKa = 3.81HH210 pKa = 6.86EE211 pKa = 4.38EE212 pKa = 3.78KK213 pKa = 10.11IYY215 pKa = 10.61RR216 pKa = 11.84CLRR219 pKa = 11.84ASSMVSFIICFGLVIAVVVVLIMNSQNHH247 pKa = 4.84GG248 pKa = 3.44

Molecular weight: 27.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0GK53|Q0GK53_9CLOS p28 OS=Strawberry chlorotic fleck-associated virus OX=399314 PE=4 SV=1
MM1 pKa = 7.16NCYY4 pKa = 9.52EE5 pKa = 5.21RR6 pKa = 11.84SDD8 pKa = 4.02LDD10 pKa = 4.3LLLGCATCAIIVFLVFICMYY30 pKa = 10.23AYY32 pKa = 8.41STRR35 pKa = 11.84PTFNKK40 pKa = 9.72PSVSRR45 pKa = 11.84SRR47 pKa = 11.84AGNSDD52 pKa = 3.41FQPPVSSYY60 pKa = 10.48ATISS64 pKa = 3.16

Molecular weight: 7.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

5482

64

2787

548.2

61.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.439 ± 0.417

2.371 ± 0.259

5.454 ± 0.335

6.074 ± 0.272

5.235 ± 0.449

5.29 ± 0.381

2.171 ± 0.209

5.144 ± 0.191

6.64 ± 0.195

9.65 ± 0.439

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.116 ± 0.122

4.341 ± 0.341

3.448 ± 0.136

1.915 ± 0.277

5.035 ± 0.196

9.723 ± 0.321

5.637 ± 0.294

8.282 ± 0.401

0.638 ± 0.083

4.396 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski