Micromonospora sp. KC723

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora; unclassified Micromonospora

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5344 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R4KSI1|A0A4R4KSI1_9ACTN Uncharacterized protein (Fragment) OS=Micromonospora sp. KC723 OX=2530381 GN=E1165_22060 PE=4 SV=1
MM1 pKa = 8.12PDD3 pKa = 3.83LPDD6 pKa = 4.84PYY8 pKa = 9.93TIRR11 pKa = 11.84HH12 pKa = 5.89FSQGNPAGPTQDD24 pKa = 4.88DD25 pKa = 4.07VPALLRR31 pKa = 11.84RR32 pKa = 11.84LADD35 pKa = 3.99TIEE38 pKa = 4.38DD39 pKa = 4.51LGPVWIQDD47 pKa = 2.97IVLHH51 pKa = 5.87NEE53 pKa = 3.0ITAEE57 pKa = 3.65GDD59 pKa = 3.69YY60 pKa = 11.49YY61 pKa = 11.55SFTVYY66 pKa = 10.36YY67 pKa = 10.45HH68 pKa = 6.83EE69 pKa = 5.33EE70 pKa = 3.79SDD72 pKa = 3.45

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R4L6F1|A0A4R4L6F1_9ACTN N5-carboxyaminoimidazole ribonucleotide mutase OS=Micromonospora sp. KC723 OX=2530381 GN=purE PE=3 SV=1
MM1 pKa = 7.22ATIPSPAIPRR11 pKa = 11.84GPTAPRR17 pKa = 11.84SPRR20 pKa = 11.84PPPRR24 pKa = 11.84RR25 pKa = 11.84PRR27 pKa = 11.84PTRR30 pKa = 11.84RR31 pKa = 11.84SRR33 pKa = 11.84PRR35 pKa = 11.84PPAGRR40 pKa = 11.84PGSRR44 pKa = 11.84GRR46 pKa = 11.84ARR48 pKa = 11.84RR49 pKa = 11.84PRR51 pKa = 11.84RR52 pKa = 11.84SRR54 pKa = 11.84CRR56 pKa = 11.84SGTRR60 pKa = 11.84TRR62 pKa = 11.84RR63 pKa = 11.84QRR65 pKa = 11.84APSSTRR71 pKa = 11.84SRR73 pKa = 11.84LPGG76 pKa = 3.43

Molecular weight: 8.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5344

0

5344

1713476

29

2609

320.6

34.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.999 ± 0.048

0.756 ± 0.009

5.96 ± 0.028

4.968 ± 0.031

2.637 ± 0.017

9.42 ± 0.033

2.155 ± 0.015

3.059 ± 0.019

1.589 ± 0.021

10.465 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.588 ± 0.012

1.838 ± 0.023

6.335 ± 0.03

2.692 ± 0.016

8.728 ± 0.034

4.609 ± 0.027

6.208 ± 0.033

9.267 ± 0.034

1.621 ± 0.015

2.105 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski