Bacillus dafuensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4401 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8Z5X8|A0A5B8Z5X8_9BACI Purine-binding chemotaxis protein CheW OS=Bacillus dafuensis OX=1742359 GN=FSZ17_09995 PE=4 SV=1
MM1 pKa = 7.49EE2 pKa = 5.8FSLCSLDD9 pKa = 3.36CALPVEE15 pKa = 4.82VILDD19 pKa = 3.92EE20 pKa = 5.22DD21 pKa = 3.49NGRR24 pKa = 11.84YY25 pKa = 8.52MIRR28 pKa = 11.84KK29 pKa = 8.55SDD31 pKa = 3.43SSGEE35 pKa = 4.16FFNTPHH41 pKa = 7.34EE42 pKa = 4.69LIQWVNNNFSAEE54 pKa = 4.16EE55 pKa = 3.94FCDD58 pKa = 3.71PSEE61 pKa = 4.08FQSMIKK67 pKa = 10.27KK68 pKa = 10.55LSDD71 pKa = 3.34YY72 pKa = 10.97LSNPNYY78 pKa = 10.89

Molecular weight: 9.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8ZAE5|A0A5B8ZAE5_9BACI Fatty acid hydroxylase OS=Bacillus dafuensis OX=1742359 GN=FSZ17_22930 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.29QPSKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.11RR12 pKa = 11.84SKK14 pKa = 9.13VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTPNGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4401

0

4401

1318923

26

7380

299.7

33.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.597 ± 0.035

0.731 ± 0.011

4.984 ± 0.031

7.58 ± 0.049

4.749 ± 0.033

6.72 ± 0.036

2.03 ± 0.019

8.496 ± 0.043

7.384 ± 0.033

9.742 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.75 ± 0.019

4.722 ± 0.027

3.59 ± 0.022

3.535 ± 0.027

3.819 ± 0.029

6.22 ± 0.029

5.224 ± 0.031

6.626 ± 0.028

1.005 ± 0.013

3.495 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski