Desulfotomaculum reducens (strain MI-1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfotomaculum; Desulfotomaculum reducens

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4J6N9|A4J6N9_DESRM N-acetyltransferase domain-containing protein OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=Dred_2229 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.53ASVDD6 pKa = 3.24QDD8 pKa = 3.78LCISCGACIDD18 pKa = 3.76TCPEE22 pKa = 3.66VFDD25 pKa = 4.44WNDD28 pKa = 2.98EE29 pKa = 3.97EE30 pKa = 4.32KK31 pKa = 10.68AYY33 pKa = 10.8AKK35 pKa = 10.38VDD37 pKa = 4.54EE38 pKa = 5.11IPDD41 pKa = 3.89GQEE44 pKa = 3.89DD45 pKa = 3.89QAKK48 pKa = 9.99DD49 pKa = 3.38AAEE52 pKa = 4.28SCPTEE57 pKa = 4.61AIRR60 pKa = 11.84ISS62 pKa = 3.33

Molecular weight: 6.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4J0F1|A4J0F1_DESRM Beta sliding clamp OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=Dred_0002 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.19QPKK8 pKa = 8.78NRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.44KK13 pKa = 10.27RR14 pKa = 11.84VHH16 pKa = 6.0GFLKK20 pKa = 10.6RR21 pKa = 11.84MSTKK25 pKa = 9.94TGRR28 pKa = 11.84NVLKK32 pKa = 10.47RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3220

0

3220

980491

36

2630

304.5

33.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.753 ± 0.053

1.155 ± 0.018

4.819 ± 0.036

6.878 ± 0.048

3.873 ± 0.034

7.477 ± 0.04

1.83 ± 0.018

7.417 ± 0.039

6.61 ± 0.039

10.13 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.021

4.303 ± 0.028

4.035 ± 0.026

3.968 ± 0.033

4.667 ± 0.033

5.473 ± 0.032

5.283 ± 0.027

7.431 ± 0.037

0.942 ± 0.017

3.235 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski