Ectothiorhodospira mobilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales;

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2413 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4T0K3|A0A1I4T0K3_ECTMO Type IV pilus assembly protein PilQ OS=Ectothiorhodospira mobilis OX=195064 GN=SAMN05421721_12613 PE=4 SV=1
MM1 pKa = 7.52NRR3 pKa = 11.84FLSFALAVMTALIPAYY19 pKa = 9.82IMAEE23 pKa = 4.02PLDD26 pKa = 4.39LADD29 pKa = 5.36APLATATSAEE39 pKa = 4.41PNVMLLIDD47 pKa = 4.11TSGSMDD53 pKa = 3.78NIIWAEE59 pKa = 4.19GYY61 pKa = 10.03DD62 pKa = 3.84PSVTYY67 pKa = 10.29PGGFDD72 pKa = 4.39ANDD75 pKa = 3.91GNIHH79 pKa = 7.16LYY81 pKa = 8.11ATKK84 pKa = 10.82DD85 pKa = 3.4FGNCGNNGEE94 pKa = 4.25WVEE97 pKa = 4.6DD98 pKa = 3.34QGGWKK103 pKa = 9.74DD104 pKa = 3.62LCLPDD109 pKa = 4.8PVGGDD114 pKa = 3.17ATRR117 pKa = 11.84YY118 pKa = 6.87TGNYY122 pKa = 9.36LNYY125 pKa = 10.17LFYY128 pKa = 10.3IYY130 pKa = 10.58TEE132 pKa = 4.95DD133 pKa = 4.95DD134 pKa = 3.21TDD136 pKa = 3.99LTDD139 pKa = 3.52GTIPTEE145 pKa = 3.82TRR147 pKa = 11.84MQVAKK152 pKa = 10.16EE153 pKa = 3.97VASQLVEE160 pKa = 3.95NTPGMRR166 pKa = 11.84FGVSQFYY173 pKa = 10.75GPTWDD178 pKa = 3.37DD179 pKa = 3.56HH180 pKa = 6.38RR181 pKa = 11.84WGEE184 pKa = 4.09GAEE187 pKa = 4.21VVAACGSSVSDD198 pKa = 3.78LTSEE202 pKa = 4.06IAGFTASTNTPLAEE216 pKa = 4.03ALYY219 pKa = 10.32EE220 pKa = 3.59ITRR223 pKa = 11.84YY224 pKa = 8.95YY225 pKa = 10.91RR226 pKa = 11.84GMDD229 pKa = 3.13AYY231 pKa = 9.62YY232 pKa = 10.53TNDD235 pKa = 3.96DD236 pKa = 3.76FASPIQYY243 pKa = 9.84RR244 pKa = 11.84CQKK247 pKa = 9.8NFTVVISDD255 pKa = 4.11GLPTYY260 pKa = 7.93DD261 pKa = 3.94TNIPGNDD268 pKa = 3.51PAEE271 pKa = 4.4SSGRR275 pKa = 11.84ALPDD279 pKa = 3.2WDD281 pKa = 4.32GLSPDD286 pKa = 4.71TDD288 pKa = 3.76PADD291 pKa = 3.85YY292 pKa = 10.56PDD294 pKa = 4.35FPQYY298 pKa = 11.31SDD300 pKa = 3.83GFQPDD305 pKa = 4.95GYY307 pKa = 9.23PSEE310 pKa = 4.5EE311 pKa = 4.75GYY313 pKa = 10.92SLYY316 pKa = 10.89LDD318 pKa = 5.87DD319 pKa = 4.46IAKK322 pKa = 10.25FGWDD326 pKa = 2.73IDD328 pKa = 3.84LRR330 pKa = 11.84TGGTDD335 pKa = 3.34DD336 pKa = 5.15AGMSFDD342 pKa = 5.59DD343 pKa = 5.17PAFEE347 pKa = 4.0QQNMGTYY354 pKa = 9.99TIGFSTANQMLEE366 pKa = 3.78DD367 pKa = 3.72AAEE370 pKa = 4.32YY371 pKa = 10.79GHH373 pKa = 7.04GEE375 pKa = 4.33YY376 pKa = 9.57LTAANAAEE384 pKa = 4.61LSAALQAAISDD395 pKa = 3.48IAARR399 pKa = 11.84SSSSASATSNTGYY412 pKa = 9.81IASGSRR418 pKa = 11.84VFQARR423 pKa = 11.84YY424 pKa = 9.91NSSNWTGQLLAFAIEE439 pKa = 4.23SDD441 pKa = 3.94VDD443 pKa = 3.86SEE445 pKa = 5.77DD446 pKa = 3.57YY447 pKa = 11.46GFLQRR452 pKa = 11.84NGPVTEE458 pKa = 4.1GAVWDD463 pKa = 3.95AAEE466 pKa = 5.06EE467 pKa = 4.11IPAAGSRR474 pKa = 11.84TIMTYY479 pKa = 10.69NGGGVPFAWTDD490 pKa = 3.31LSTAQQSALGSEE502 pKa = 4.75DD503 pKa = 3.19VLSYY507 pKa = 11.22LRR509 pKa = 11.84GDD511 pKa = 3.46QSQEE515 pKa = 3.72VSSGGSFRR523 pKa = 11.84NRR525 pKa = 11.84DD526 pKa = 3.58ALLGDD531 pKa = 4.06IVNSNPVFMGAPPFNYY547 pKa = 9.52PDD549 pKa = 3.84DD550 pKa = 4.26WNGDD554 pKa = 3.45TTEE557 pKa = 5.02PEE559 pKa = 4.01DD560 pKa = 3.82ASPYY564 pKa = 10.79SSFAVNQNDD573 pKa = 4.5RR574 pKa = 11.84TPMIYY579 pKa = 10.3VGANDD584 pKa = 3.79GMLHH588 pKa = 6.42GFDD591 pKa = 4.66AEE593 pKa = 4.38TGEE596 pKa = 4.08EE597 pKa = 3.79RR598 pKa = 11.84MAYY601 pKa = 9.96VPGSVFEE608 pKa = 4.57NLDD611 pKa = 3.57EE612 pKa = 5.82LVDD615 pKa = 3.13TDD617 pKa = 3.68YY618 pKa = 11.72AHH620 pKa = 7.85RR621 pKa = 11.84YY622 pKa = 8.26FVDD625 pKa = 4.88GSPTIADD632 pKa = 3.75AYY634 pKa = 11.09FDD636 pKa = 4.61GSWHH640 pKa = 5.61TVLLGGLNKK649 pKa = 10.28GGQGIYY655 pKa = 10.77ALDD658 pKa = 3.48VTTATFSEE666 pKa = 4.89GNADD670 pKa = 4.04STVLWEE676 pKa = 4.38FTDD679 pKa = 4.63ADD681 pKa = 4.42DD682 pKa = 4.67VDD684 pKa = 4.04LGYY687 pKa = 9.28TFSQPNIVRR696 pKa = 11.84LHH698 pKa = 5.72NGQWAAIFGNGYY710 pKa = 10.24NNTRR714 pKa = 11.84ADD716 pKa = 3.7GDD718 pKa = 3.67ASTTGHH724 pKa = 6.12AVLYY728 pKa = 9.9IVSIEE733 pKa = 4.21DD734 pKa = 3.12GSLIRR739 pKa = 11.84KK740 pKa = 8.99IDD742 pKa = 3.57TGAGTATTPNGLATVSPVDD761 pKa = 3.19VDD763 pKa = 2.83GDD765 pKa = 4.35YY766 pKa = 11.1IVDD769 pKa = 3.54RR770 pKa = 11.84VYY772 pKa = 11.24AGDD775 pKa = 3.58LQGNLWRR782 pKa = 11.84FDD784 pKa = 4.29LDD786 pKa = 4.97DD787 pKa = 4.83GNPNQWGIPFSQGQTHH803 pKa = 6.41EE804 pKa = 4.61PLFSACSGTPCDD816 pKa = 5.01SNRR819 pKa = 11.84QPITVRR825 pKa = 11.84PEE827 pKa = 3.28VGRR830 pKa = 11.84APNKK834 pKa = 9.46PGAVMLYY841 pKa = 10.04FGTGKK846 pKa = 10.44YY847 pKa = 10.33LEE849 pKa = 4.75NGDD852 pKa = 4.25GNVADD857 pKa = 4.41TPQMQRR863 pKa = 11.84FYY865 pKa = 11.59GVLDD869 pKa = 3.64EE870 pKa = 6.03DD871 pKa = 4.72LPANQFDD878 pKa = 4.14TLVPGDD884 pKa = 4.42LLEE887 pKa = 4.17QEE889 pKa = 4.68ILVEE893 pKa = 4.13SSFNVEE899 pKa = 4.37GNSHH903 pKa = 5.63SLRR906 pKa = 11.84VTSDD910 pKa = 3.27YY911 pKa = 11.52TLGSEE916 pKa = 4.68DD917 pKa = 3.68KK918 pKa = 10.83GWYY921 pKa = 8.96IDD923 pKa = 4.05LAPPGTGSVGEE934 pKa = 4.25IAVTAPLLRR943 pKa = 11.84AGRR946 pKa = 11.84VFFTTLIPGEE956 pKa = 4.88DD957 pKa = 3.53PCQPGGQGWLMEE969 pKa = 4.68LDD971 pKa = 4.57AINGGRR977 pKa = 11.84LSYY980 pKa = 11.27SPFDD984 pKa = 4.68LNDD987 pKa = 3.47DD988 pKa = 3.9TSFDD992 pKa = 3.79DD993 pKa = 3.9GDD995 pKa = 4.17YY996 pKa = 9.96VTVTVEE1002 pKa = 3.79EE1003 pKa = 4.89GEE1005 pKa = 4.31GEE1007 pKa = 4.4EE1008 pKa = 4.11IQVPASGKK1016 pKa = 9.79RR1017 pKa = 11.84LDD1019 pKa = 3.67VGGADD1024 pKa = 3.68MPGVMSGEE1032 pKa = 3.95GSEE1035 pKa = 4.35FKK1037 pKa = 10.95YY1038 pKa = 10.85ISGDD1042 pKa = 3.37DD1043 pKa = 3.48GLQVVRR1049 pKa = 11.84EE1050 pKa = 4.17NPGPEE1055 pKa = 4.01SMGRR1059 pKa = 11.84MSWEE1063 pKa = 3.69QLQQ1066 pKa = 3.47

Molecular weight: 114.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4R1B2|A0A1I4R1B2_ECTMO Delta-aminolevulinic acid dehydratase OS=Ectothiorhodospira mobilis OX=195064 GN=SAMN05421721_10669 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLHH11 pKa = 6.3RR12 pKa = 11.84KK13 pKa = 6.84RR14 pKa = 11.84THH16 pKa = 5.74GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84KK29 pKa = 8.98VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.28GRR39 pKa = 11.84ARR41 pKa = 11.84LCPP44 pKa = 3.71

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2413

0

2413

789774

29

1816

327.3

36.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.315 ± 0.063

0.936 ± 0.018

5.763 ± 0.04

6.769 ± 0.053

3.173 ± 0.029

8.788 ± 0.05

2.637 ± 0.027

4.087 ± 0.035

2.171 ± 0.036

11.46 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.426 ± 0.022

2.081 ± 0.025

5.668 ± 0.041

3.869 ± 0.032

8.925 ± 0.048

4.288 ± 0.025

4.736 ± 0.03

7.349 ± 0.044

1.34 ± 0.02

2.221 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski